均勻設(shè)計法研究八角油β-環(huán)糊精包合物的制備工藝

1、均勻設(shè)計法研究八角油-環(huán)糊精包合物的制備工藝         【摘要】 目的 研究-環(huán)糊精對八角油的包合作用。方法 以揮發(fā)油包合率為指標(biāo)，應(yīng)用3因素6水平的均勻設(shè)計法，篩選出八角油包合物的最佳制備條件，即溫度、研磨時間、樣品與-CD的比例。結(jié)果實驗得出最佳條件：當(dāng)溫度為35.0，研磨時間為4.0 h，八角茴香油與-CD的比        【摘要】  目的研究-環(huán)糊精對八角油的包合作用。方法 以揮發(fā)油包合率為指標(biāo)，應(yīng)用3因素6水

2、平的均勻設(shè)計法，篩選出八角油包合物的最佳制備條件，即溫度、研磨時間、樣品與-CD的比例。結(jié)果實驗得出最佳條件：當(dāng)溫度為35.0，研磨時間為4.0 h，八角茴香油與-CD的比例113.0（ mlg）時，包合率為81.4%；紅外測定包合作用是否完全。結(jié)論優(yōu)選得到的工藝結(jié)果穩(wěn)定可行。 【關(guān)鍵詞】  八角油； -環(huán)糊精； 包合物制備； 均勻設(shè)計Abstract:ObjectiveTo study the inclusion complex of Star anise oil with cyclodextrin. Methods3 factors and 6 levels of conditi

3、ons for the preparation of the inclusion complex were selected by the uniform design. The ratio of Star anise oil in the inclusion complex was used as criterion in these experiments. The inclusion complex was made by pestling method. ResultsThe best conditions of the inclusion complex were that: the

4、 ratio of Star anise oil and -Cyclodextrin was 1:13.0(mL/g), and grinding for 4.0 hours at 35.0. The ratio of volatile oil in the inclusion complex was about 81.4%, and the inclusion complex was identified by IR. ConclusionThe optimized process is stable and highly efficient.   Key wordsSt

5、ar anise oil；-Cyclodextrin；  Inclusion process；Uniform design    Guangxi is a Chinese autonomous province, located in a subtropical zone, south west of China. In particular, it has extensive resources of different plants. Among them, there is a special and commercial product, ess

6、ential oil, which is widely recognized. Essential oils are the volatile, fragrant substances residing in the flowers, fruits, leaves, and roots of many plants1.Most of them are a complex mixture of chemical constituents，including nitrogen and sulfur-containing molecules, aromatic chemicals, and terp

7、enes    The following oils are the most famous and are found in large quantity in Guangxi. Eucalyptus oi1 has been employed in ointments as a topical counter-irritant and together with menthol as an inhalation1.Star anise oil is the main product in the fruits of Illicium verum. The pl

8、ant contains remarkable essential oil, which is used as flavoring and medicine to control caught2.The related content of trans-Cinnamaldehyde in Guangxi cinnamon oil was 11.58%, higher than that in Yunnan cinnamon oil. These two provinces are the main producing areas of cinnamon oil in China 3. 

9、;   Star anise oil is separated from the fruits of Illicium verum Hook.f. This plant is used as flavorings as well as Chinese herbal medicine. In Star anise oil, anethole(structure below) is the major component which has structures as the Cis & trans isomers occurring in nature. Remark

10、ably, the Trans isomer is always being more abundant. Star anise oil has a distinct effect on raising leucocytes (the white blood-cells), so in American patent, it has been applied in the remedy for anti-leukemia. And it also has been used as poultry feed additive composition4. However, the effectiv

11、eness decreases with long time storage, and both physical and chemical characteristics change relatively.-CyclodextrinX(structure above) has been of interest in numerous industries including the pharmaceutica1 field. This is due to their particular structures which impart interesting physiochemical

12、properties. Today -CD is known for their ability to molecularly encapsulate a wide variety of the drugs into their hydrophobic cavity, resulting in the enhancement of water solubility and drug dissolution's rate1. The results of physi cochemical tests showed that after inclusion the solubility a

13、nd stability of paprika pigment, the binding ability of -cyclodextrin for inclusion complex with guest curcumin, was improved obviously57.So it is considered as a good supplementary material for injection8.    Up to date, although the uniform design has been widely used in many synthe

14、sis and separation fields9,no reports related with the inclusion complex of Star anise oil and-cyclodextrin, by three factors and six levels uniform design, has been available. However, by using this practical design, the value of Star anise oil would definitely enhance and would benefit many pharma

15、ceutical and commercial industries.1 Reagents    -Cyclodextrin: was obtained from Pharmaceutical Manufacturing Factory.Shanghai;Star anise oil: was abstracted from the fruits of Illicium verum Hook.f. All the other chemicals and reagents used were of the reagent grade obtained commerc

16、ially.2  Methods2.1  Use Uniform Design for the experimentsAmong many factors that effect the preparation of the complex, the temperature, the ratio of oil and CD, and the time to mix two compounds, are most important10.Hence, U*（63）three factors and six levels of the uniform design was considered. To see details, in table 1.2.2  Researches in the inclusion complex2.3 IR diffraction manifestationSince IR is a very use