DHEA-Prasterone-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDHEACat. No.: HY-14650CAS No.: 53-43-0Synonyms: Prasterone; Dehydroisoandrosterone;Dehydroepiandrosterone分式: CHO分量: 288.42作靶點(diǎn): Androgen Receptor; Endogenous Metabolite作通路: Others; Metabolic Enzyme/Protease儲(chǔ)存式: Powder -20C 3 year

2、s4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 50 mg/mL (173.36 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 3.4672 mL 17.3358 mL 34.6717 mL5 mM 0.6934 mL 3.4672 mL 6.9343 mL10 mM 0.3467 mL 1.7336 mL 3.4672 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn)

3、請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄清的?chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (8.67 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (8.

4、67 mM); Clear solution1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (8.67 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 DHEA (Prasterone)最豐富的類 醇激素之。 DHEA通過多種信號傳導(dǎo)途徑介導(dǎo)其作，并通過轉(zhuǎn)化成雄激素和雌激素衍物（例如，雄激素，雌激素，7和7 DHEA (Prasterone)，以及7和7表雄甾酮衍物）通過其特異性受體起作。IC50

5、 & Target Human Endogenous Metabolite體外研究 DHEA (Prasterone) is an effective antiapoptotic factor, reversing the serum deprivation-induced apoptosis inprostate cancer cells (DU145 and LNCaP cell lines) as well as in colon cancer cells (Caco2 cell line). DHEA(Prasterone) significantly reduces serum de

6、privation-induced apoptosis in all 3 cancer cell types, quantitatedwith the APOPercentage assay (apoptosis is reduced from 0.5870.053 to 0.1420.0016 or 0.0590.002after treatment for 12 hours with DHEA or NGF, respectively; n=3, P50: 11.23.6 nM and 12.42.2 nM inDU145 and Caco2 cells, respectively) 1.

7、 DHEA (Prasterone) is the principal sex steroid precursor inhumans and can be converted directly to androgens. DHEA (Prasterone) (1 M) causes a dose-dependentinhibition of Chub-S7 proliferation, as assessed by thymidine incorporation assays. DHEA (Prasterone)treatment inhibits expression of the key

8、glucocorticoid-regulating genes H6PDH (100 nM) and HSD11B1 (1M) in differentiating preadipocytes in a dose-dependent manner. In keeping with this finding, DHEA(Prasterone) treatment (1 M) results in a marked reduction in 11-HSD1 oxoreductase activity (1 M) anda concurrent increase in dehydrogenase a

9、ctivity at the highest DHEA dose used (25 M DHEA) indifferentiated adipocytes 2.體內(nèi)研究 DHEA (Prasterone) in the diet (0.45 % w/w) of male B6 mice (groups of five mice) treated for 8 weeks led tosignificant decreases in body temperature compared with mice fed the control AIN-76A diet. A similarcomparis

10、on indicated that control and pair-fed mice are also significantly different. Animals fed DHEA(Prasterone) have significantly lower temperatures than mice fed the control diet 26/29 times tested; micepair fed to those on the DHEA (Prasterone) diet are less affected, with 8/29 values significantly lo

11、wer than inmice fed AIN-76A ad libitum. The temperatures of mice fed DHEA (Prasterone) or pair fed to DHEA(Prasterone) are significantly different 21/29 times tested. Body weights are significantly greater in mice fedthe control diet than in mice fed DHEA or pair fed to DHEA (Prasterone). Food intak

12、e (grams per day) fromcages are averaged for each week (n=7), except for Week 9 (n=3). The amount of food intake is significantlydecreased in mice fed DHEA (Prasterone). By design, mice pair fed to DHEA (Prasterone) ate about thesame amount. Thus, it appears that DHEA (Prasterone) reduces body tempe

13、rature by food restriction and bya separate mechanism 3.PROTOCOLCell Assay 2 Chub-S7 preadipocytes and human primary preadipocytes are seeded into a 24-well plate at densities 1105and 2.5105 respectively. Following overnight culture, medium is supplemented with DHEA, androstenediol,2/3 Master of Sma

14、ll Molecules 您邊的抑制劑師www.MedChemEor DHEA (Prasterone) (0-100 M). Following 24-, 48-, or 72 h incubation, cell proliferation is assessed byincubation with radiolabeled thymidine (0.2 Ci/well) for the final 6 h of culture. Proteins are precipitated withTCA, and cells are scraped in NaOH. The respective

15、 content of radiolabeled nuclear material in the resultinglysates is analyzed by scintillation counting 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice 3Administration 3 Mice are fed Purina Lab Chow until the start of experiments (Day 0).

16、Groups of five mice are then fedpelleted AIN-76A diet containing either no additive or DHEA (0.45% w/w) between 0900 and 1000 hr. Dietsare stored at 4C for no longer than six months to maintain optimal activity. Mice are given the diets adlibitum, except for mice that are pair fed to mice treated wi

17、th DHEA (Prasterone). The amounts of AIN-76Adiet the pair-fed mice received are determined by the weight of food consumed by the DHEA-fed mice on adaily basis. Body weights (grams) are measured at different time points starting at Day 1 and ending at Day59. Daily food intakes (grams per day) are det

18、ermined by weighing the food consumed per cage of five mice.The meanSEM values are calculated for weeks 1 to 8 (n=7); week 9 had only 3 days.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Toxicol Appl Pharmacol. 2019 Jun 5:114612.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Anagnostopoulou V, et al. Differential effects of dehydroepiandrosterone and testosterone in prostate and colon cancer cell apoptosis:the role of nerve grow