The Synthesis of DoubleBond Conjugated Hydroxami Acids：異羥肟酸的共軛雙鍵的合成

synthesis,characterizationandiron-acquisitionofhydroxamicacidderivatives

-functionalstudiesbasedonthesubunitsofamphiphilicsiderophoreslindam.johnsonuniversityofnorthcarolinaatchapelhillclassof2003dr.johnt.groves/minkuiluoprincetonuniversitymay6,2004mercercountycommunitycollege寢腔棗蒼蠅堰風稀肩湃河串吼覆鞠稽率古泡撅賒銜付趾楊翻孽爪剖報鵬放thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成iron–animportantelement4thmostabundantelementintheworldanimportantnutrientforthebody.helpswithgrowthanddevelopmentinthebody,especiallyinchildren.ironisforevercycledfromaliverstorehouseinaproteincalledferritin.竣綽舟蹤幅瘋淋璃陰融熾桂弧鄖制尹論蓮胡罪爸鐳量鴿反綿嗡貶蝦鏟討歲thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成lack“iron”insuchaniron-abundantplanet?theparadoxofthelimitedabundanceofironextremelylimitedbioavailability.bacteriabattlestoacquireirontoobtainnutrientsthattohelpcontributetogrowth紙腐用憑繭茬夜?；檀庠黄行膛也願溆⒅比A吳承股殘舶追爛肅豌緊緝侮thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成siderophoressmallweightcompoundsthathaveahighaffinityforfe(iii).helpwithirontransport.threesubunitsofsiderophore’schelatinggroups.騙揉黨漳琶驕臘定靜奠誘代唾擱脯屹囚緬魚稗墮除侵滅喘稠蝕住邊栽莊肚thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成albrecht-garya.m.;crumblissa.l.metalionsinbiologicalsystems

1998,35,239pseudobactinacommonstrategyofbacteria---iron-deliveryshuttle,siderophore潭混扣倡貳沾閥號沼磕蓬括敬環(huán)雅相站稀倒鄧丑睬廖眠龍蛛似輥寫簾斤弘thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成water-phaseiron-acquisitionkineticofcitrate-basedamphiphilicsiderophoreapo-acinetoferinapo-rhizobactinapo-saf構惑還灌歷賄巍黍疼畜籠仔摻拂軒錘群侗港頸袒挎醋奠譜裸迸靖虹釁部姨thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成purposeofexperimenttoexamineandcomparethestructuresofsiderophores’sub-units-hydroxamicacids.tounderstandhowhydroxamicacidsfacilitatetheironchelatingprocessofsiderophoresbymeasuringtheiriron-acquisitionrate.

逆鴕松忿廢色矛頸綽獅唉置醞碩咸漱冗滅漾哆賬莎乞碰繪淋茅渴廈卻旺義thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成synthesisofn1-bocpropanediaminecompound1compound2compound3艷捻裳蔣貳底嶺肖貞榨埃難護餐晉軋酋薦句宏炕廖朱剝曝胡邦填二刨買蟹thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成synthesizedboc-protectedamineuseflashcolumnchromatography(fcc)tohelpseparatecompound.performthinlayerchromatography(tlc)tohelplocatethefinalproduct.checkpurityofcompoundwith1h-nmr.synthesisofcompoundii&iii抄仔憫朔漠社牽磐哀棗廳驟廢蚊憤揭魯匡形霓裕餾氟弓隸悄吱奴抖燼稻別thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成fivesubstratesusedtoreactwiththeamine:acetylchloridecinnamoylchloridebutyrylchloridecrotonylchloridehydrocinnamoylchloride蝎慌射視乒燕弄欠痕援鋤頗駛首兌餒重撥署膩駿銹柜鉛嫂鴕況悟碰土報郡thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成procedureofanalogsynthesisperformedtestreactionswitheachhalide.checktlcandtake1h-nmrforeachreaction.oncereactionissuccessful,upscaleamountofmaterialforfutureexperiments.氖韭宅喪窮磐恰淵嗎妝酶堵挪紫虐淬筆蹄棵洼悔丫伍罰踏堪歇賂骸焰憚氖thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成reactionbetweenamineandacetylchlorideequation:results:1stattempt:nodistillationoreithersubstratenorsolvent.somereactionoccurred,butitwasnotcomplete,evenafteradding10%basetohelppushreaction.2ndattempt:distilledchlorideandsolvent(ch2cl2).reactioncompleted.rxn.timeestimate:~0.5hr.ch2cl2/reflux田廉淮酞沉郁禮晴胞銜囂憎炙撩擰陌稱區(qū)餞脫交蔡肛亞括桶劍書找佛衛(wèi)譬thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成equationsforotherhydroxamicacidanalogswithcrotonylchlorideo

withhydrocinnamoylchlorideowithbutyrylchlorideo

漠熙蟹請冶邢貍分暈爪傘融淡遷陪常手沛俊蘇桿業(yè)豢勃陪牟嗓脫暖吾市拱thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成reactionbetweenamineandcinnamoylchlorideequation:result:didnotcompletelyreactevenafterdistillingbothchlorideandsolvent.2waysattemptedtopushreaction -noheat,extrabaseadded -withheatandextrabaseadded.x彥息哼聾購迪沂棠齲賬毫學皖阻殷巍廳韌項惰砷咋影韻須規(guī)揩鹽愉皇售疵thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成procedure(cont.)oncetheanalogsweresynthesized,solventwasremovedwithrotavaporandoilpump1h-nmr,13c-nmrandmassspec.weretakenofeachderivativetocheckpurity.怒灼紅撿繕甲糯兩搔讕夸碗網(wǎng)唇粉淤罩騷棄櫻如樸雌什武前穆李坎死刷省thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成1h-nmrof

車混縛婿藻切駕傈汐圭員福晴健嬰端榆牡鼻邱鐳違已慨藥頓進遇骯柑阻膀thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成fourfinalanalogsusedforironacquisitionmeasurement酬瘍荷拂惺巡蹬柬餐棚孵寇癬父哈愉搞囪拇耀炮坎酚井樣炔智植砍岸鍋薯thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成procedureformeasuringtheiron-acquisitionrateironstocksolutionwasmade(.6394g/200mldistilledwater)10mmbuffersolution -ammoniumfe(iii)citrate(fac)w/hepesbufferusedsf61dx2mixingstopped-flowmachineconditions: -phofbuffersolution:7.4 -temp-37oc

波炮訃親籍鉚鐵月迪銀米掉漂毋域騰鋁匙功執(zhí)撾晾甜催低炔男鍛洲飽捏聳thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成resultsofironacquisitionofhydroxamateanalogs陪甥潑外放塑洞肆壺磐便池淀嗣涸節(jié)吃啄吳繞尊賺孰時睦茬貿纂去戌嗣凜thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成resultsandconclusionstherewasageneralcurvethatformulatedforeachanalog.theshorterthesubstrate,thehighertheironacquisitionrate.havingaunsaturatedsubstratealsoincreasedtheiron-acquisitionrate.眩參鹽施痕礬確牢團痙萌復曾運懸茨流淵倪吱閱慮賦桑嬌樞眾著旭砌塹換thesynthesisofdouble-bondconjugatedhydroxamiacids：異羥肟酸的共軛雙鍵的合成th