LncRNA全國講座-課件

LncRNA全國講座“Learner&Nontrivial&Confidence”-RNA全國技術中心技術LncRNA全國講座“Youstartprojectsandyouhavenoideawhattoexpect.It’sthatkindofchallengethatIenjoyverymuchasascientist.”LncRNA全國講座CircRes2012LncRNA全國講座TherapeuticapproachInclinicaltrialsApprovedtherapy

EnzymereplacementtherapyYESYESGenetherapyYESYESNuclearreceptorligandsYESYESChromatinorDNAmodulatorsYESYESArtificialtranscriptionfactors YESNOMicroRNAmodulators YESNOCis-actinglncRNAmodulators NONOTrans-actinglncRNAmodulators NONO非タンパクコードRNAを利用した醫(yī)薬開発GenomeNetworkProtein2Protein1Protein3Protein4Protein5Protein5PGene2ProteinXGene10ncRNA3ncRNA4Gene5Gene6DrugGene7Protein10Protein12Gene3Protein7Protein7Protein8Protein9Gene1Protein11Protein13ncRNA1ncRNA6Gene4Gene11Gene9ncRNA2Gene8DiseaseNewTargetofMedicineMutsumiKanamori-KatayamaPh.D.YoshihideHayashizakiM.D.,Ph.D.RIKENGenomeSciencesCenterGenomeExplorationResearchGroupLncRNA全國講座LncRNA全國講座LncRNA全國講座Ryanet.al,BioEssays29:288–299(2007)LncRNA>200nt結構類似mRNA具有外顯子/內含子低蛋白編碼潛力LncRNA全國講座LncRNA全國講座LncRNA全國講座CabiliMN.IntegrativeannotationofhumanlargeintergenicnoncodingRNAsrevealsglobalpropertiesandspecificsubclasses.GenesDev.2011Sep15;25(18):1915-27LncRNA全國講座LncRNA全國講座LncRNA全國講座LncRNA全國講座ncRNAPANDA募集轉錄因子NF-YA，啟動細胞凋亡通路LncRNA全國講座Cell2011ncRNA-andPc2Methylation-DependentGeneRelocationbetweenNuclearStructuresMediatesGeneActivationProgramsLncRNA全國講座NatureMedicine2010ExpressionofanoncodingRNAiselevatedinAlzheimer’sdiseaseanddrivesrapidfeed-forwardregulationofβ-secretaseexpression早發(fā)老年性癡呆病中BACE1-AS高表達導致BACE1mRNA穩(wěn)定性增高，蛋白積聚，體現(xiàn)了ncRNA的毒性效應BACE1BACE1-ASLncRNA全國講座拷貝數(shù)染色體定位表達LncRNA全國講座LncRNA全國講座IF:31.027LncRNA全國講座LncRNA全國講座LncRNA全國講座芯片結果聚類圖測序結果散點圖backNorthernblotlncRNA-DC在免疫細胞中的表達情況lncRNA-DC在DC/mono細胞群中的表達情況backChIP-Seq與ChIP-PCR結果back熒光素酶報告分析，驗證轉錄因子結合區(qū)域LncRNA全國講座backLncRNA全國講座干擾結果過表達結果backLncRNA全國講座抑制DC細胞分化back通過STAT3的RIP實驗驗證LncRNA全國講座Lnc-DC與STAT3相互結合back進一步驗證Pulldown和RIP的結果LncRNA全國講座back結果表明，lnc-DC的3′末端序列（265-417）與STAT3的C端結合（583-770）。STAT3的活化依賴C端Tyr705的磷酸化。LncRNA全國講座back研究結果表明，S3I-201處理的細胞系與RNAi干擾后的細胞系在基因表達差異方面非常相似。LncRNA全國講座LncRNA全國講座高通量檢測生物信息分析表達、定位驗證上游驗證功能驗證下游驗證LncRNA全國講座LncRNA全國講座LncRNA全國講座LncRNA全國講座Affymetrix?TranscriptomeAnalysisConsole2.0(TAC2.0)Software

LncRNA全國講座LncRNA全國講座LncRNA全國講座IF:10.143LncRNA全國講座IF:4IF:11.139LncRNA全國講座LncRNA全國講座上游下游反義鏈正義鏈LncRNA全國講座LncRNA全國講座LncRNA全國講座

Hepatology2011LncRNA全國講座通過網絡找到調控lncRNA最多的轉錄因子同時找到能被不同轉錄因子調控最多的lncRNA使用德國Biobase數(shù)據庫商業(yè)版數(shù)據庫LncRNA全國講座LncRNA全國講座AffymetrixPromoter1.0RArrayAffymetrixHTA2.0LncRNA全國講座藍色線表示兩點之間的關系需要計算綠色線表示兩點之間的關系通過轉錄因子的預測得到LncRNA全國講座LncRNA全國講座IF:38.597LncRNA全國講座深灰色：激活淺灰色：抑制LncRNA全國講座I