25%呋蟲胺·吡唑醚菌酯懸浮種衣劑的HPLC分析

25%呋蟲胺·吡唑醚菌酯懸浮種衣劑的HPLC分析HPLCAnalysisof25%Furametpyr·pyrrolethioneSuspensionSeedDressingAgent1.IntroductionFurametpyr·pyrrolethioneisawidelyusedinsecticideinagriculturalpractices.Itiseffectiveagainstabroadrangeofpestssuchasaphids,whiteflies,andthrips.Inordertoenhanceitsapplicationefficiency,furametpyr·pyrrolethioneisformulatedintoa25%suspensionseeddressingagent,allowingforeasyandefficientdistributiononseeds.HPLC(High-PerformanceLiquidChromatography)iscommonlyutilizedfortheanalysisofactiveingredientsinpesticideformulations.ThispaperaimstodescribetheHPLCanalysisofthe25%furametpyr·pyrrolethionesuspensionseeddressingagent.2.MaterialsandMethods2.1Materials-25%furametpyr·pyrrolethionesuspensionseeddressingagent-HPLC-gradeacetonitrile-HPLC-gradewater-Analyticalstandardsoffurametpyr·pyrrolethione-HPLCapparatuswithUVdetector-HPLCcolumn(C18,250mmx4.6mm,5μm)2.2HPLCAnalysis2.2.1PreparationofStandardSolutionsAstocksolutionoffurametpyr·pyrrolethionewaspreparedbydissolvingtheanalyticalstandardsinasuitablesolvent(e.g.,acetonitrile)ataknownconcentration(e.g.,1000μg/mL).Aseriesofworkingstandardsolutionswerethenpreparedbyappropriatedilutionofthestocksolutionwithacetonitrile.2.2.2SamplePreparationAsuitableamountofthe25%furametpyr·pyrrolethionesuspensionseeddressingagentwasaccuratelyweighedandtransferredtoavolumetricflask.Acetonitrilewasadded,andthemixturewassonicatedfor30minutesandthenfilteredthrougha0.45μmsyringefilterpriortoinjection.2.2.3HPLCConditionsTheHPLCanalysiswasperformedusinganisocraticelutionwithamobilephaseconsistingofacetonitrileandwater(70:30,v/v).Theflowratewassetat1mL/min,andtheinjectionvolumewas10μL.Thecolumntemperaturewasmaintainedat25°C,andthedetectionwavelengthwassetat220nm.3.ResultsandDiscussionTheretentiontimeoffurametpyr·pyrrolethioneontheHPLCcolumnwasfoundtobeapproximately3.8minutes.Thecalibrationcurvewasconstructedbyplottingthepeakarearatiosoftheanalytetotheinternalstandardagainstthecorrespondingconcentrationsoffurametpyr·pyrrolethione.Thelinearityofthecalibrationcurvewasevaluatedbycalculatingthecorrelationcoefficient(r^2).Themethodwasvalidatedforselectivity,linearity,accuracy,precision,androbustness.Selectivitywasconfirmedbyanalyzingblanksamplesandverifyingtheabsenceofinterferencepeaksattheretentiontimeoffurametpyr·pyrrolethione.Thelinearityofthemethodwasassessedbyanalyzingstandardsolutionsatdifferentconcentrationswithinthesuitablerange.Accuracywasdeterminedbyanalyzingrecoverysamplesatthreedifferentfortificationlevels,andprecisionwasevaluatedbycalculatingtherelativestandarddeviation(RSD)ofreplicateinjections.4.ConclusionInconclusion,areliableandrobustHPLCmethodwasdevelopedfortheanalysisofthe25%furametpyr·pyrrolethionesuspensionseeddressingagent.Themethoddemonstratedgoodselectivity,linearity,accuracy,andprecision.Itcanbeappliedforroutinequalitycontrolanalysisoffurametpyr·pyrrolethioneformulations.FurtherstudiescanbeperformedtovalidatethemethodondifferentHPLCsystemsandevaluateitsstability-indicating