基于修飾組學(xué)研究乳酸化調(diào)控微擬球藻產(chǎn)油的分子機(jī)制

基于修飾組學(xué)研究乳酸化調(diào)控微擬球藻產(chǎn)油的分子機(jī)制目錄TOC\o"1-3"\h\u摘要 [9]，研究表明這種修飾在表觀遺傳調(diào)控和基因轉(zhuǎn)錄中扮演著重要角色REF_Ref163374508\r\h[10]REF_Ref163375753\r\h[24]。除了組蛋白外，非組蛋白乳酸化也可能調(diào)節(jié)自噬、蛋白質(zhì)折疊、信號(hào)轉(zhuǎn)導(dǎo)、細(xì)胞分裂、DNA損傷修復(fù)和代謝等多種細(xì)胞過(guò)程。然而，乳酸化在海洋微擬球藻中的具體作用尚未被充分理解。在本研究中，我們通過(guò)在氮?jiǎng)儕Z（ND）和氮充足（NR）條件下對(duì)海洋微擬球藻的蛋白質(zhì)組和乳酸化修飾進(jìn)行高通量分析，建立了海洋微擬球藻的乳酸化組數(shù)據(jù)庫(kù)。共鑒定出5203個(gè)蛋白質(zhì)，其中1245個(gè)表現(xiàn)出顯著的差異調(diào)控。相比之下，在ND和NR條件下，379個(gè)蛋白質(zhì)中有868個(gè)乳酸化位點(diǎn)。研究還發(fā)現(xiàn)，在脂質(zhì)代謝等關(guān)鍵代謝途徑中，乳酸酶活性在ND和NR條件下發(fā)生了大規(guī)模的重編程。這些發(fā)現(xiàn)與之前在布魯氏錐蟲、灰霉病菌和水稻中的研究結(jié)果一致，乳酸化被確認(rèn)為影響組蛋白修飾、基因轉(zhuǎn)錄和碳濃縮機(jī)制（CCM）的重要因素REF_Ref163375983\r\h[25]。此外，蛋白質(zhì)的乳酸化水平也受到培養(yǎng)基中氮含量的影響。與NR條件下相比，在ND條件下生長(zhǎng)的海洋微擬球藻表現(xiàn)出更高程度的乳酸化修飾。這些關(guān)于氮脅迫如何影響乳酸化的數(shù)據(jù)，將為理解微藻產(chǎn)油機(jī)制提供新的表觀遺傳學(xué)視角。

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