常用釀酒酵母菌株基因型

1、Commonly used strainsThis page describes some of the most commonly used yeast lab strains. Much of the information is taken fromF. Sherman (2002)Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include: Mortimer and Johnston(1986) Genetics

2、 113:35-43 - thoroughly describes the genealogy of strain S288C van Dijken et al.(2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains Winzeler et al.(2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identi

3、ty between common lab strainsS288CGenotype:MATSUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes:Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy ofHAP1, it is not a good strain for mitochondria

4、l studies. It has an allelic variant ofMIP1which increases petite frequency. S288C strains aregal2- and they do not use galactose anaerobically.The S288C genome was recently resequenced at theSanger Institute.References:Mortimer and Johnston(1986) Genetics 113:35-43.Sources:ATCC:BY4743Genotype:MATa/

5、his31/his31 leu20/leu20 LYS2/lys20 met150/MET15 ura30/ura30Notes:Strain used in thesystematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant ofMIP1which increases petite frequency. See Brachmann et al.

6、 reference for details.References:Brachmann et al.(1998) Yeast 14:115-32.Sources:Biosystems:YSC1050FY4Genotype:MATaNotes:Derived from S288C.References:Winston et al.(1995) Yeast 11:53-55.Brachmann et al.(1998) Yeast 14:115-32.FY1679Genotype:MATa/ura3-52/ura3-52 trp163/TRP1 leu21/LEU2 his3200/HIS3 GA

7、L2/GALNotes:Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Winston et al.(1995) Yeast 11:53-55.Sources:EUROSCARF:10000DAB972Genotype:MATX2180-1B trp10rho0Notes:Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SG

8、D. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.References:Olson MV et al.(1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.Sources:ATCC:A364AGenotype:MATaade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONotes:Used in the systematic sequencing project, the sequence

9、stored in SGD.References:Hartwell(1967) J. Bacteriol. 93:1662-1670.Sources:ATCC:XJ24-24aGenotype:MATaho HMa HM ade6 arg4-17 trp1-1 tyr7-1 MAL2Notes:Derived from, but not isogenic to, S288CReferences:Strathern et al.(1979) Cell 18:309-319DC5Genotype:MATaleu2-3,112 his3-11,15 can1-11Notes:Isogenic to

10、S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Broach et al.(1979) Gene 8:121-133X2180-1AGenotype:MATaSUC2 mal mel gal2 CUP1Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated

11、 X2180References:Mortimer and JohnstonSources:ATCC:YNN216Genotype:MATa/ura3-52/ura3-52 lys2-801amber/lys2-801amberade2-101ochre/ade2-101ochreNotes:Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).References:Sikorski RS and Hieter P(1989) Genetics 1

12、22:19-27.Sobel and Wolin(1999) Mol. Biol. Cell 10:3849-3862.YPH499Genotype:MATaura3-52 lys2-801_amber ade2-101_ochre trp1-63 his3-200 leu2-1Notes:Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note thattrp1-63, unliketrp1-1, does not delete adjacen

13、tGAL3UAS sequence and retains homology toTRP1selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P(1989) Genet

14、ics 122:19-27.Sobel and Wolin(1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW(1984) Mol Cell Biol 4(8):1440-8.Sources:ATCC:YPH500Genotype:MATura3-52 lys2-801_amber ade2-101_ochre trp1-63 his3-200 leu2-1Notes:MAT strain isogenic toYPH499except at mating type locus. Derived from the diploid

15、 strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P(1989) Genetics 122:19-27.Sobel and Wolin(1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW(1984) Mol Cell Biol 4(8):1440-8.Sources:ATC

16、C:YPH501Genotype:MATa/MATura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-63/trp1-63 his3-200/his3-200 leu2-1/leu2-1Notes:a/ diploid isogenic toYPH499andYPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia Univ

17、ersity), which is congenic with S288C.References:Sikorski RS and Hieter P(1989) Genetics 122:19-27.Sobel and Wolin(1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW(1984) Mol Cell Biol 4(8):1440-8.Sources:ATCC:Sigma 1278BNotes:Used in pseudohyphal growth studies.Detailed notesabout the sigm

18、a strains have been kindly provided by Cora Styles.Granek and Magwene, PLoS Genet. 2010 Jan 22;6(1):e, established that certain lineages of the Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim

19、15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology.Annotation of the Sigma1278b genome and information about the systematic deletion collection can be foundhere.SK1Genotype:MATa/HO gal2 cupScan1RBIONotes:Commonly used for studying sporulation or mei

20、osis. Canavanine-resistant derivative.The SK1 genome was sequenced at theSanger Institute.References:Kane SM and Roth J.(1974) Bacteriol. 118: 8-14Sources:ATCC:CEN.PK (aka CEN.PK2)Genotype:MATa/ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his31/his31 MAL2-8C/MAL2-8C SUC2/SUC2Notes:CEN.PK

21、possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delays glucose-induced loss of stress resistance (Vanhalewyn et al., 1999;Dumortier et al., 20

22、00).References:van Dijken et al.(2000) Enzyme Microb Technol 26:706-714Sources:EUROSCARF:30000DW303Genotype:MATa/MAT leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 phi+Notes:W303 also contains abud4mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In add

23、ition, the original W303 strain contains therad5-535allele. As S288c, W303 has an allelic variant ofMIP1which increases petite frequency.The W303 genome was sequenced at theSanger Institute.References:W303 constructed by Rodney Rothstein (seedetailed notesfrom RR and Stephan Bartsch).bud4info:Voth e

24、t al.(2005) Eukaryotic Cell, 4:1018-28.rad5-535info: Fan et al. (1996) Genetics 142:749Sources:Biosystems:YSC1058W303-1AGenotype:MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15Notes:W303-1A possesses aybp1-1mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensi

25、tivity to oxidative stress.References:W303 constructed by Rodney Rothstein (seedetailed notesfrom RR and Stephan Bartsch).ybp1-1info:Veal et al.(2003) J. Biol. Chem. 278:30896-904.Sources:Biosystems:YSC1058W303-1BGenotype:MAT leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15References:W303 constru

26、cted by Rodney Rothstein (seedetailed notesfrom RR and Stephan Bartsch).Sources:Biosystems:YSC1058W303-K6001Genotype:MATa; ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho:HO:CDC6 (at HO), cdc6:hisG, ura3:URA3 GAL-ubiR-CDC6 (at URA3)References:K6001 was developed byBobola et alin Kim

27、Nasmyths lab (PMID: ), and has become a common model in yeast aging research (PMID: ). Its genome has been sequenced by Timmermann et al (PMID: )D273-10BGenotype:MATmalNotes:Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in num

28、erious laboratories for mitochondrial and related studies and for mutant screens. Good respirer thats relatively resistant to glucose repression.References:Sherman, F.(1963) Genetics 48:375-385.Sources:ATCC:24657FL100Genotype:MATaReferences:Lacroute, F.(1968) J. Bacteriol. 95:824-832.Sources: ATCC:

29、28383SEY6210/SEY6211Genotype:MATa/MATleu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-200/his3-200 trp1-901/trp1-901 ade2/ADE2 suc2-9/suc2-9 GAL/GAL LYS2/lys2-801Notes:SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It

30、is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation.References:Robinson et al.(1988) Mol Cell Biol 8(11):4936-48Sources:ATCC:SEY6210Genotype

31、:MATleu2-3,112 ura3-52 his3-200 trp1-901 suc2-9 lys2-801; GALNotes:SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sher

32、man, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al.(1988) Mol Cell Biol 8(11):4936-48Sources:ATCC:96099SEY6211Genotype:MATaleu2-3,112 ura3-52 his3-200 trp1-901 ade2-101 suc2-9; GALNotes:SEY6211 is a MATa haploid constructed by Scott Emr and h

33、as been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al.(1988) Mol Cell Bi

34、ol 8(11):4936-48Sources:ATCC:96100JK9-3dThere are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:Genotypes:JK9-3daMATaleu2-3,112 ura3-52 rme1 trp1 his4JK9-3d has the same genotype as JK9-3da with the exception of the MAT locusJK9-3da/ is homozygous for all markers except mating

35、 typeNotes:JK9-3d was constructed by Jeanette Kunz while in Mike Halls lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab

36、, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.References:Heitman et al.(1991a) Science 253(5022):905-9 andHeitman et al.(1991b) Proc Natl Acad Sci U S A 88(5):1948-52RM1