Hexadimethrine-bromide-1-5-Dimethyl-1-5-diazaundecamethylene-polymethobromide-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHexadimethrine bromideCat. No.: HY-112735CAS No.: 28728-55-4Synonyms: 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide分式: CHBrN分量: 404.27作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C

2、1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) H2O : 125 mg/mL (309.20 mM)DMSO : 5 mg/mL (12.37 mM; Need ultrasonic and warming)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 2.4736 mL 12.3680 mL 24.7359 mL5 mM 0.4947 mL 2.4736 mL 4.9472 mL10 mM 0.2474 mL 1.2368 mL 2.4736 mL請根據(jù)產(chǎn)品在不同溶劑中

3、的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄清的?chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 0.5 mg/mL (1.24 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-C

4、D in saline)1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 0.5 mg/mL (1.24 mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 0.5 mg/mL (1.24 mM); Clear solution; Need warmingBIOLOGICAL ACTIVITY物活性 Hexadimethrine bromide種聚合物，可增強(qiáng)逆轉(zhuǎn)錄病毒轉(zhuǎn)導(dǎo)。體外研究 Hexadimethrine bromide inhibit

5、s human mesenchymal stem cell proliferation during lentiviral transduction.Hexadimethrine bromide is considered non-toxic at low concentrations, but has been found to negativelyaffect cell proliferation in some cell types at concentrations greater than 10 g/mL. Trypsinized cells exposedto Hexadimeth

6、rine bromide are visibly larger in size when viewed under the microscope 1.PROTOCOLCell Assay 1 Trypsinized cells are washed and resuspended in medium at 2104 cells/mL with or without rhFGF-2 (finalconcentration=10 ng/mL, PeproTech) and with or without Hexadimethrine bromide at a final Hexadimethrin

7、ebromide concentration of 1, 4, or 8 g/mL. The different conditions are seeded in 96-well plates at 1103cells in 50 L per well in triplicate and cultured at 37C, 5% CO2. After 6, 9, or 24 hr, the medium is changedand subsequent medium changes occurred every 3-4 days with 50 L of FGF-2 medium (10 ng/

8、mL). Platesare harvested on day 4, 7, 14, and 21 by removing the medium and placing the plates in the -80C freezeruntil the day of analysis. The CyQUANT assay is then performed on the wells 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) A

9、dv Funct Mater. 2019 Mar. Cell Signal. 2019 Mar 5. pii: S0898-6568(19)30038-5.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Lin P, et al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891.McePdfHeightCaution: Product has not been fully validated for medica