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1、精選優(yōu)質(zhì)文檔-傾情為你奉上精選優(yōu)質(zhì)文檔-傾情為你奉上專心-專注-專業(yè)專心-專注-專業(yè)精選優(yōu)質(zhì)文檔-傾情為你奉上專心-專注-專業(yè)聲明:The study complied with the declaration of Helsinki and was approved by the local research ethics committee. All subjects gave written informed consent.All animal experimentation was approved by the St. Louis VA Animal Care Committee

2、and conducted in conformity with the Guiding Principles for Research Involving Animals and Human Beings.The study was carried out with institutional review board approval. Informed consent was obtained from patients to perform for study purposes.The protocol was approved by the Institutional Review

3、Board (IRB) of The Mount Sinai School of Medicine.This study was conducted in accordance with the United States Department of Agriculture Animal Welfare Act, and the University of Missouri Animal Care and Use Committee (ACUC) approved the protocol. All animals were housed and cared for at the Univer

4、sity of Missouri-Columbia animal research facilities.The study protocols and informed consents were approved by the Institutional Review boards and Institutional Biosafety committees of all participating institutions. (TS)The study design and study protocols, approved by the US Food and Drug Adminis

5、tration (FDA), were performed in accordance with FDA regulations CFR Title21,applicable International Conference on Harmonization guidelines, and National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules.This study was approved by our institutional ethical committee.T

6、he study was approved by the institutional review board of the Mayo Foundation, and all subjects gave written informed consent. The study was designed and conducted entirely by the study team without industry support. Randomization schedules were prepared by study statisticians.The Animal Care Commi

7、ttee of the Toronto General Research Institute approved all procedures performed on animals. All experiments were performed according to the Guide to the Care and Use of Experimental Animals of the Canadian Council on Animal Care, and the Guide for the Care and Use of Laboratory Animals (NIH publica

8、tion 85-23, revised 1985).This study was performed with informed consent, HIPAA compliance, and institutional review board approval.All other study participants gave written informed consent. The study was approved by the local research ethics committee.The study protocols and informed consents were

9、 approved by the Institutional Review boards and Institutional Biosafety committees of all participating institutions. (TS)The study design and study protocols, approved by the US Food and Drug Administration (FDA), were performed in accordance with FDA regulations CFR Title21,applicable Internation

10、al Conference on Harmonization guidelines, and National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules.標(biāo)題:Statistical analysisStatistical considerationsStatistics employedStatistics and data analysisData analysisStatistical MethodsStatistical analysis of the dataSta

11、tistics樣本含量估計(jì)On the basis of preliminary estimates of the corresponding standard deviations, we determined that 30 subjects would be required in each group for the study to have a statistical power of 90% to detect clinically meaningful differences between groups.Prior to the study, power calculatio

12、ns were made based on systolic and diastolic blood pressure values. Published results of blood pressure in subjects with OSA commonly reported a standard deviation of 14 mmHg for systolic and 7 mmHg for diastolic blood pressure. With this information, a power calculation indicated that we would need

13、 32 patients per group to detect a 10-mmHg difference in systolic blood pressure and a 5-mmHg difference in diastolic blood pressure with a power of 80% at a 0.05 significance level.統(tǒng)計(jì)描述:Data are reported as meanSD.Data are expressed as meanSD.Data are presented as meanSEM for continuous variables,

14、with n indicating the number of patients.Normally distributed data were presented as means standard error of the mean, logarithmically transformed data as the geometric mean (95% confidence interval for the mean), skewed data as the median (interquartile range), and categorical data as the number (p

15、ercentage).假設(shè)檢驗(yàn)方法:Statistical testing utilized non-parametric tests with MannWhitney U test and KruskalWallis test for continuous variables and 2 test and Fishers exact test for categorical variablesCategorical variables were compared using Fishers exact test and, for continuous variables, the non-p

16、arametric MannWhitney test.Differences between groups in (variables) were assessed with analysis of variance (ANOVA) followed by Bonferroni or Fisher LSD post hoc tests. was/were not normally distributed and therefore the Mann-Whitney U test was used. ANOVA and t testing were used for other paramete

17、rs.Statistical comparisons for percentages were performed using 2 analysis in each case. Comparisons between means were performed using a two-tailed t-test.Multivariable analysis utilized logistic/linear regression with backward/forward/stepwise variable selection.Comparisons of continuous variables

18、 were made by means of the unpaired Students t test. Bonferronis correction was applied for multiple comparisons. Correlations between targeted contrast ultrasound signal and blood volume were made by linear regression analysis.Differences in T-cell responses between patient groups or time points we

19、re tested by the Fisher exact test or the Wilcoxon signed-rank test. Correlations were tested by the nonparametric Spearmans rank test.Unpaired t-tests and x2 tests were used to study the clinical profile of EC donors. Two-tailed paired t-tests were used to compare NAC-treated and matchedcontrol unt

20、reated cells. Unpaired t-tests were used to compare data in EC responding positively or not to the antioxidant treatment.In order to predict whether risk factors for CVD could have an impact on the response to NAC (dependent dichotomic variable), we used logistic regression models. Only univariate a

21、nalyses could be performed because of the small n (26 patients: 11 with negative response and 15 with positive).Time-course and multigroup analyses were carried out using analysis of variance (ANOVA).Bonferroni and Newman-Keuls post test analysis was used to detect differences between groups. A t te

22、st with Welch correction was used to compare groups at a single time point.Dichotomous variable group differences between patients with and without old microbleeds were analyzed using the Fisher exact test. Group differences for continuous variables between patients with and without old microbleeds

23、were analyzed using the Wilcoxon rank-sum test.Comparisons within groups to investigate differences between pre- and postaugmentation were undertaken using paired t-tests after appropriate analyses had shown that the Central Limit Theorem applies and that parametric statistics could be used. Analysi

24、s of variance was used to investigate differences between groups numbering more than two. To investigate association between variables, Spearmans rank correlation statistic was used.We then used multivariable logistic regression to adjust for potential confounding factors.Statistically significant d

25、ifferences before and after treatment were evaluated using the paired Students t test, with statistical significance established at p0.05.Proportions were compared by 2. Correlations were performed using the Spearman rank test.We performed multivariable unconditional logistic regression analyses to

26、estimate the adjusted odds ratios (AOR) and 95% confidence interval (CI) for each risk factor using maximum likelihood estimation. All AORs (95% CIs) were adjusted for age, sex, race, education level, cigarette smoking, alcohol consumption, diabetes mellitus, family history of cancer, and HBV/HCV in

27、fection. The final model was chosen on the basis of biological plausibility and the lowest -2 log likelihood function.Differences between groups in (variables) were assessed with analysis of variance (ANOVA) followed by Bonferroni or Fisher LSD post hoc tests. Correlation between and was calculated

28、using the Pearson product-moment correlation.確定檢驗(yàn)水準(zhǔn):P-values 0.05 were considered statistically significant and p values of 0.05) were compared using with a significant difference determined at P0.05.This null hypothesis was rejected with statistical significance assigned for values of P0.05.Differences were consid

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