Oenothein-B-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEOenotheinBCat.No.:HY-N7765CASNo.:104987-36-2分?式:C??H??O??分?量:1571.1作?靶點(diǎn):HCV;Bacterial;Apoptosis;Fungal作?通路:Anti-infection;Apoptosis儲(chǔ)存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性O(shè)enotheinB?種?聚體?環(huán)鞣花單寧，具有?泛的藥理活性，包括抗氧化、抗炎、抗真、抗HCV和抗腫瘤特性。OenotheinB?種有效且特異性的聚(ADP-核糖)糖?解酶抑制劑。體外研究OenotheinB(15-45μM;12-72hours)hasadose-andtime-dependentinhibitionrateeffectonA549cellsintherangeof12hours,24hours,36hours,48hours,60hoursand72hoursofexposure[1].OenotheinB(15-45μM;24hours)effectivelyinhibitstheproliferationofA549cellsbyinducingapoptosisandarrestingcellsatG1stage[1].OenotheinB(15-45μM;24hours)notonlyincreasesthelevelofintracellularreactiveoxygenspecies(ROS),butalsoinducestheupregulationofintracellularapoptotictriggers(cleavagecaspase-3,PARP,cytochromeclevelinthecytosol,Bax)[1].A549cells[1]15μM,30μMand45μM24hoursArrestedcellsintheG1phase.A549cells[1]15μM,30μMand45μM24hoursBAX,p53,cytochromec(cytoplasm)andPARPwereunregulatedsignificantly;Anti-apoptoticBcl-2wasdecreasedsignificantlyinaconcentration-dependentmanner.CellViabilityAssay[1]CellLine:A549cellsConcentration:15μM,30μMand45μMIncubationTime:12hours,24hours,36hours,48hours,60hoursand72hoursResult:EffectivelyinhibitedtheproliferationofA549cells.ApoptosisAnalysis[1]1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECellLine:A549cellsConcentration:15μM,30μMand45μMIncubationTime:24hoursResult:InducedapoptosisinA549cells.CellCycleAnalysis[1]CellLine:A549cellsConcentration:15μM,30μMand45μMIncubationTime:24hoursResult:ArrestedcellsintheG1phase.WesternBlotAnalysis[1]CellLine:A549cellsConcentration:15μM,30μMand45μMIncubationTime:24hoursResult:BAX,p53,cytochromec(cytoplasm)andPARPwereunregulatedsignificantly;Anti-apoptoticBcl-2wasdecreasedsignificantlyinaconcentration-dependentmanner.體內(nèi)研究OenotheinB(100-300mg/kg;p.o.)hastheabilitytoreduceneuroinflammationinthebrainduringsystemicinflammationinICRmice[3].REFERENCES[1].XiaodongPei,etal.OenotheinBinhibitshumannon-smallcelllungcancerA549cellproliferationbyROS-mediatedPI3K/Akt/NF-κBsignalingpathway.ChemBiolInteract.2019Jan25;298:112-120.[2].SatoruTamura,etal.OenotheinB,dimerichydrolysabletannininhibitingHCVinvasionfromOenotheraerythrosepala.JNatMed.2019Jan;73(1):67-75.[3].SatoshiOkuyama,etal.OenotheinBsuppresseslipopolysaccharide(LPS)-inducedinflammationinthemousebrain.IntJMolSci.2013May7;14(5):9767-78.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedical