細(xì)環(huán)病毒從實(shí)驗(yàn)室到臨床（課堂PPT）

細(xì)環(huán)病毒：從實(shí)驗(yàn)室到臨床Torquetenovirus:thehumanviromefrombenchtobedside文獻(xiàn)匯報(bào)D.Focosietal.ClinicalMicrobiologyandInfection22(2016)589-5931TTV:Transfusiontransmittedvirus

orTorquetenovirusFromtorquesandtenuis,Latinfor‘necklace’and‘thin,’respectively輸血后肝炎（肝中病毒載量是血中的10-100倍，并且與血清ALT升高相關(guān)）【現(xiàn)已否定該結(jié)論】Humanviromeatleast29majorspecies細(xì)環(huán)病毒：首個(gè)被發(fā)現(xiàn)的單鏈環(huán)狀DNA病毒2TTVprevalence>95%inmostcasesindependentofage,socioeconomic

standingandhealth

conditions來(lái)源：胎盤(pán)、呼吸道、輸血分布：T淋巴細(xì)胞現(xiàn)有臨床應(yīng)用：TTVasaMarkerofAnthropicPollutionTTVasaMarkerofImmuneStatusOrphanvirus~孤兒病毒（非病原性病毒）3檢測(cè)水源是否被污染：現(xiàn)有方法為檢測(cè)細(xì)菌，但是很容易出現(xiàn)假陽(yáng)性某些情況下即使細(xì)菌檢測(cè)為陰性也不能排除被污染檢測(cè)病毒更準(zhǔn)確TTV分布廣泛：醫(yī)院空氣、物件表面均可存在TTVasaMarkerofAnthropicPollutionTTVasaMarkerofImmuneStatus免疫功能完整性：theabilitytomountaprotectiveimmuneresponseagainstanantigenicstimulation.現(xiàn)有檢測(cè)：抗原特異性，抗原非特異性；檢測(cè)繁瑣，檢測(cè)方法缺乏標(biāo)準(zhǔn)化TTV與CD8+57+T淋巴細(xì)胞比例成負(fù)相關(guān)對(duì)抗病毒藥不敏感4TTVasaMarkerofImmuneStatusexcessive(toomanyopportunisticinfectionsandcancers)inadequate(toomanyepisodesofcellularacuterejection)Maintenanceimmunosuppression5WhyIsTTVNotYetPartofaDiagnosticToolKit?現(xiàn)有研究不足無(wú)法培養(yǎng)無(wú)抗體檢測(cè)試劑無(wú)動(dòng)物模型PCR方法缺乏標(biāo)準(zhǔn)化（引物設(shè)計(jì)）特異性引物非特異性引物6TakehomemessagesTTVisatypeofOrphanvirus,prevalence>95%inmostcasesTTVreplicatesmainlyinTlymphocytesInsensitivetocurrentantiviraldrugs

TTVasaMarkerofImmun