Hydrocortisone-hemisuccinate-Hydrocortisone-21-hemisuccinate-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEHydrocortisonehemisuccinateCat.No.:HY-B1402CASNo.:2203-97-6Synonyms:Hydrocortisone21-hemisuccinate分?式:C??H??O?分?量:462.53作?靶點(diǎn):GlucocorticoidReceptor;InterleukinRelated作?通路:Immunology/Inflammation儲(chǔ)存?式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:250mg/mL(540.51mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.1620mL10.8101mL21.6202mL5mM0.4324mL2.1620mL4.3240mL10mM0.2162mL1.0810mL2.1620mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(4.50mM);Clearsolution1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(4.50mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(4.50mM);ClearsolutionBIOLOGICALACTIVITY?物活性Hydrocortisonehemisuccinate(Hydrocortisone21-hemisuccinate)，?種糖?質(zhì)激素，?服活性的甾體抗炎劑(SAID)。Hydrocortisonehemisuccinate抑制IL-6和IL-3的?物活性，IC50值分別為6.7和21.4μM。Hydrocortisonehemisuccinate可?于潰瘍性結(jié)腸炎(UC)的研究。IC50&TargetIL-3IL-6IL-6IL-36.7μM(IC50)21.4μM(IC50)體外研究HydrocortisonehemisuccinateinhibitsIL-6andIL-3bioactivity,withIC50sof6.7and21.4μM,respectively,andshowsnocytotoxiceffectsonIL-6-independentMH60cells[3].Hydrocortisonehemisuccinate(0.12-60μM;72h)inhibitsphytohemagglutinin(PHA)responseinperipherallymphocytes(PBL)andT-lymphocytescultures[3].體內(nèi)研究Hydrocortisonehemisuccinate(30mg/kg;p.o.twicedailyfor5d)reducestheweightlossandincreasesthefoodintakeinmice[2].AnimalModel:MaleSprague-Dawleyrats(200-220g,10-11weeks)areinducedcolitis[2]Dosage:30mg/kgAdministration:P.o.twicedailyfor5daysResult:Significantlydecreasedthediseaseactivityindex(DAI)scoresandmyeloperoxidase(MPO)activitycomparedtothe2,4,6-trinitrobenzenesulfonicacid(TNBS)group.Increasedthebodyweight.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?BiomedPharmacother.2022Jun7;152:113243.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].KangBS,et,al.Inhibitoryeffectsofanti-inflammatorydrugsoninterleukin-6bioactivity.BiolPharmBull.2001Jun;24(6):701-3.[2].YouYC,et,al.InvitroandinvivoapplicationofpH-sensitivecolon-targetingpolysaccharidehydrogelusedforulcerativecolitistherapy.CarbohydrPolym.2015Oct5;130:243-53.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE[3].LanghoffE,et,al.Theimmunosuppressivepotencyinvitroofphysiologicalandsyntheticsteroidsonlymphocytecultures.IntJImmunopharmacol.1987;9(4):469-73.McePdfHeightCaution:Producthasnotbeenfullyvalidatedf