Zinc-Protoporphyrin-Zn-II-protoporphyrin-IX-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEZincProtoporphyrinCat.No.:HY-101193CASNo.:15442-64-5Synonyms:Zn(II)-protoporphyrinIX;ZnPP;ZincProtoporphyrin-9分?式:C??H??N?O?Zn分?量:626.02作?靶點(diǎn):ReactiveOxygenSpecies;EndogenousMetabolite;Apoptosis作?通路:Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB;Apoptosis儲(chǔ)存?式:-20°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:20.83mg/mL(33.27mM;ultrasonicandwarmingandheatto80°C)0.2MNaOH:2.5mg/mL(3.99mM;ultrasonicandadjustpHto14withNaOH)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.5974mL7.9870mL15.9739mL5mM0.3195mL1.5974mL3.1948mL10mM0.1597mL0.7987mL1.5974mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。BIOLOGICALACTIVITY?物活性ZincProtoporphyrin(Zn(II)-protoporphyrinIX)?種具有?服活性的，競(jìng)爭(zhēng)性的?紅素加氧酶-1(HO-1)抑制劑，顯減弱間苯三酚(PG)對(duì)H2O2的保護(hù)作?。ZincProtoporphyrin可?作個(gè)體孕婦和?童缺鐵的篩查標(biāo)志物，也?于評(píng)估?群鐵狀態(tài)與?紅蛋?濃度的組合。ZincProtoporphyrin具有抗癌活性。1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEIC50&TargetHumanEndogenousMetabolite體外研究ZincProtoporphyrin(Zn(II)-protoporphyrinIX;5μM;72hours)causesthefractionoflateapoptoticandnecroticcellsincreasingfrom10.9%incontrolsto30.4%after72h[3].ZincProtoporphyrin(1.25-40μM;48or72hours)exertscystostatic/cytotoxiceffectsagainsttumorcells[3].ZincProtoporphyrin(2.5,5μM;48or72hours)resultsindose-andtime-dependentreductionofcellsinG1phaseofthecellcycle[3].ZincProtoporphyrin(1.25-40μM;48hours)leadstoaccumulationofcleaved(active)caspase-3[3].ApoptosisAnalysis[3]CellLine:C-26cellsConcentration:5μMIncubationTime:72hoursResult:Thefractionoflateapoptoticandnecroticcellsincreasedfrom10.9%incontrolsto30.4%after72h.CellCytotoxicityAssay[3]CellLine:C-26andMDA-MB231cellsConcentration:1.25,2.5,5,10,20,40μMIncubationTime:48or72hoursResult:Exertedcystostatic/cytotoxiceffectsagainsttumorcells.CellCycleAnalysis[3]CellLine:C-26cellsConcentration:2.5,5μMIncubationTime:48or72hoursResult:Resultedindose-andtime-dependentreductionofcellsinG1phaseofthecellcycle.WesternBlotAnalysis[3]CellLine:C-26cellsConcentration:1.25,2.5,5,10,20,40μMIncubationTime:48hoursResult:Leadedtoaccumulationofcleaved(active)caspase-3.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE體內(nèi)研究ZincProtoporphyrin(12.5,25,50mg/kgfori.p.;12.5,50mg/kgforp.o.;fromday7to19)exertsdose-dependentantitumoreffectsmanifestedbytheretardationoftumorgrowth[3].AnimalModel:BALB/cmiceinoculatedwithC-26cells[3]Dosage:12.5,25,50mg/kgfori.p.;12.5,50mg/kgforp.o.Administration:IPorPO;fromday7to19Result:Exerteddose-dependentantitumoreffectsmanifestedbytheretardationoftumorgrowth.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?BoneRes.2022Mar9;10(1):26.?RedoxBiol.2021Dec1;48:102203.?FreeRadicBiolMed.2022Aug23;S0891-5849(22)00558-5.?BiomedPharmacother.2022Aug;152:113255.?OxidMedCellLongev.2022Apr25;2022:3846217.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].ParkC,etal.ProtectiveEffectofPhloroglucinolonOxidativeStress-InducedDNADamageandApoptosisthroughActivationoftheNrf2/HO-1SignalingPathwayinHaCaTHumanKeratinocytes.MarDrugs.2019Apr13;17(4).[2].MwangiMN,etal.DiagnosticutilityofzincprotoporphyrintodetectirondeficiencyinKenyanpregnantwomen.BMCMed.2014Nov26;12:229.[3].NowisD,etal.ZincprotoporphyrinIX,ahemeoxygenase-1inhibitor,demonstratespotentantitumoreffectsbutisunabletopotentiateantitumoreffectsofchemotherapeuticsinmice.BMCCancer.2008Jul11;8:197.McePdfHeight