CWP232228-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemECWP232228Cat.No.:HY-18959CASNo.:1144044-02-9分?式:C??H??N?Na?O?P分?量:717.62作?靶點:β-catenin;Wnt作?通路:StemCell/Wnt儲存?式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性數(shù)據(jù)體外實驗H2O:62.5mg/mL(87.09mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.3935mL6.9675mL13.9350mL5mM0.2787mL1.3935mL2.7870mL10mM0.1393mL0.6967mL1.3935mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。BIOLOGICALACTIVITY?物活性CWP232228?種?效的選擇性Wnt/β-catenin信號通路抑制劑，在細胞核中，拮抗β-catenin與T細胞因?(TCF)結(jié)合。CWP232228通過抑制乳腺癌和肝癌?細胞(CSCs)的?長來抑制腫瘤的形成和轉(zhuǎn)移??毒性[1]。IC50&TargetWnt/β-catenin[1]1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE體外研究CWP232228(0.01-100μM;48hours)inhibitscellproliferationwithIC50valuesare2and0.8μMinmouse(4T1)andhuman(MDA-MB-435)breastcancercelllines,respectively[1].CWP232228(0.01-10μM;48hours)inhibitscellproliferationwithIC50sof2.566,2.630,and2.596μMinHep3B,Huh7andHepG2cells,respectively[2].CellProliferationAssay[1]CellLine:Mouse(4T1)andhuman(MDA-MB-435)breastcancercelllinesConcentration:0.01,0.1,1,10,100μMIncubationTime:48hoursResult:IC50swere2and0.8μMfor4T1andMDA-MB-435celllines,respectively.CellProliferationAssay[2]CellLine:HepatocellularcarcinomacelllinesHepG2,Huh7,andHep3BConcentration:0.01,0.1,0.5,1,5,10μMIncubationTime:48hoursResult:IC50swere2.566,2.630,and2.596μMforHep3B,Huh7andHepG2cells,respectively.體內(nèi)研究CWP232228(100mg/kg,administeredi.p.;daily;21daysformicebearing4T1celltumors;60daysformicebearingMDA-MB-435celltumors)resultsinasignificantreductionintumorvolume[1].AnimalModel:7-week-oldfemaleBalb/candNOD/SCIDmicebearing4T1orMDA-MB-435celltumors[1]Dosage:100mg/kgAdministration:Administeredi.p.;daily;21daysformicebearing4T1celltumors,60daysformicebearingMDA-MB-435celltumorsResult:Treatmentresultedinasignificantreductionintumorvolume.戶使?本產(chǎn)品發(fā)表的科研?獻?CellDeathDiscov.2022Oct1;8(1):404.?FrontPhysiol.2021Mar3;12:626248.?ResearchSquarePreprint.2020Jun.Seemorecustomervalidationsonwww.MedChemEREFERENCES2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE[1].JangGB,etal.Wnt/β-CateninSmall-MoleculeInhibitorCWP232228PreferentiallyInhibitstheGrowthofBreastCancerStem-likeCells.CancerRes.2015Apr15;75(8):1691-702.[2].KimJY,etal.Oncotarget.2016Apr12;7(15):20395-409.CWP232228targetslivercancerstemcellsthroughWnt/β-cateninsignaling:anoveltherapeuticapproachforlivercancertreatment.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformed