分子生物學試卷英文

西南大學生物技術學院《 》課程試題og卷? 學年第學期期末考試考試時間120分鐘考核方式開卷筆試學生類別本科人數(shù)98適用專業(yè)或科類生物技術年級05級題號 .二三四五六七八九十合計得分簽名閱卷須知：閱卷用紅色墨水筆書寫，得分用阿拉伯數(shù)字寫在每小題題號前，用正分表示，不得分則在題號前寫0；大題得分登錄在對應的分數(shù)框內；統(tǒng)一命題的課程應集體閱卷，流水作業(yè)；閱卷后要進行復核，發(fā)現(xiàn)漏評、漏記或總分統(tǒng)計錯誤應及時更正；對評定分數(shù)或統(tǒng)分記錄進行修改時，修改人必須簽名。 特別提醒：學生必須遵守課程考核紀律，違規(guī)者將受到嚴肅處理。IDefinethemeaningofthesefollowingterms(30points,3ptsperterm)meltingtemperature(Tm)Cvalueparadoxexonsemi-conservationreplicationcDNAlibrarygelshiftassaypromoteropenreadingframe(ORF)genefamilyexpressedsequencetag(EST)IIFillinblank(10points,1ptsperblank)Thesimplestcloningvectoratleastcontains,andorielements.2.ThediagrambelowshowstwoRNAmolecules.MoleculeIisprocessedtogivemoleculeII.Answerthefollowingquestionsaboutthesetwomolecules:Wheredoesthisconversionhappen? WhattypeofRNAmoleculeismoleculeI? WhattypeofRNAmoleculeismoleculeII? Nametheregions/structuresindicatedbythearrows:IIISelecttherightanswer(20points,2ptsperquestion;Selectthebestanswerforeachquestion.)TOC\o"1-5"\h\zThebondbetweenaphosphategroupandtheribosesugargroupsinRNAiscalledwhichofthefollowing?( )(A)Phosphodiesterbond(B)Glycosidicbond(C)Peptidebond (D)AmidebondWhichenzymedoesnotplayaroleinDNAreplication?( )(A)DNApolymerase (B)Helicase(C)Single-strandedbindingprotein(D)GuanyltransferaseSplicingistheprocessthatdoeswhichofthefollowing?( )RemoveintronsandconserveexonsRemoveexonsandconserveintronsRemovemutatedregionsofprimarytranscriptRNAAddmultipleadenosinebasestotheendofaprimaryRNAtranscriptTheidealspacingbetweenthe-10and-35regionsofaprokaryoticpromoteris:( )bp.(A)14 (B)15(C)16 (D)17TOC\o"1-5"\h\zThe5'cappingprocesscreateswhattypeoflinkage?( )(A)5'-3' (B)3'-5'(C)5'-5' (D)3'-3'Codonsthatcodeforthesameaminoacidarecalled:( )(A)Synonyms (B)Similar(C)Degenerates (D)ComplementsWhichofthefollowingisnotacharacteristicofatRNAmolecule?( )(A)Darm (B)Betaarm(C)Tarm (D)Anti-codonarmWhatsignalstheendoftranscription?( )(A)Stopcodon (B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsoutThestartcodonis:( )(A)AUU (B)UAA(C)AUG (D)UAGWhichofthefollowingisthesequenceofthe-10regionofprokaryoticpromoters?( )(A)TATAAT (B)TTGACA(C)TATA (D)CAAIVAnalyticalquestions(30points,6ptsperquestion)Describebrieflytheattenuationofthetrpoperon.DescribetheprincipleofSanger’senzymicmethodforDNAsequencing.WhatarethebasicfeaturesofDNAdoublehelixdeducedbyWatsonandCrick?PleasedrawaschematicstructureofTTPinthefollowingspace.Youmaywritejusttheletter"T"forthebase.OnthefollowingagarosegelwithmarkerDNAasindicated:Placeaplus(+)signonthepositivepoleofthegelandaminus(-)signonthenegativepole.Drawthebandorbandsthatyouwouldseeifyoudigesteda3kbplasmidwithEcoRIandranittotheleftofthemarker.AssumetheplasmidhasaMCSwiththefollowingrestrictionsites: