全自動血細胞分析顯微鏡復(fù)檢規(guī)則解讀及復(fù)檢方法-文檔在線預(yù)覽

全自動血細胞分析顯微鏡復(fù)檢規(guī)則及復(fù)檢方法解讀復(fù)檢規(guī)則制定的原則:、復(fù)檢規(guī)則制定的依據(jù)及驗證結(jié)果四、復(fù)檢后報告方式三、復(fù)檢規(guī)則及復(fù)檢方法復(fù)檢規(guī)則制定的原則-1.根據(jù)該儀器對細胞形態(tài)的識別能力。-2.復(fù)檢規(guī)則涉及的參數(shù)該儀器均能提供。-3.復(fù)檢范圍應(yīng)涵蓋儀器的所有參數(shù)及形態(tài)學(xué)特征。?4.在確保病理細胞不漏檢、保證篩選質(zhì)量的基礎(chǔ)上盡量降低復(fù)檢率，使患者能在較短的時間內(nèi)獲得正確的檢驗報告。?5.假陰性是制定復(fù)檢規(guī)則的關(guān)鍵參數(shù)，具有診斷意義的重要參數(shù)不能出現(xiàn)假陰性，其他參數(shù)的假陰性率應(yīng)＜5%。?6.在較低假陰性率的前提下降低假陽性率，假陽性率過高會增加工作量，延遲報告發(fā)放時間。?7.白血病患者及臨床醫(yī)生提出閱片需求的樣本必須鏡檢。復(fù)檢規(guī)則制定的依據(jù)及驗證結(jié)果?1.根據(jù)2005年國際血液學(xué)復(fù)檢專家組推薦的41條自動血細胞分析和分類復(fù)檢規(guī)則。?2.根據(jù)本實驗室的具體情況及SysmexXE-2100的性能特點，參考國際41條復(fù)檢規(guī)則，制定了適合本實驗室的17條顯微鏡復(fù)檢規(guī)則。3.驗證結(jié)果：真陽性率10.6%假陽性率16.8%真陰性率69.6%假陰性率3%復(fù)檢率27.4%復(fù)檢規(guī)則及復(fù)檢方法4-7X109/L;7-9X109/L;10-12X109/L13-18X109/LHGBRBC:RBC直方圖出現(xiàn)雙峰或多峰WBC:WBC彡30123涂片鏡檢彡200g/L或＜60g/L，涂片鏡檢。,涂片鏡檢。0X109/L或＜2.5X109/L的初診患者WBC數(shù)量的估計方法2-4/HP4-6/HP6-10/HP10-12/HP復(fù)檢規(guī)則及復(fù)檢方法圖異趴滿明『尾部抬高觀值的縱’涂片矗方檢，如結(jié)果不符，應(yīng)人工計數(shù)。-PLT數(shù)量的估計方法：-如PLT數(shù)為80X109/L，RBC數(shù)為4.00X1012/L,油鏡觀察5-10個視野每400個RBC可見8個左右的PLT。提示PLT聚集，必需鏡檢。即使未提示PLT聚集，但PLT較低，特別注意觀察片尾。explorerfiascopendingUpperLowervalidatecp&nmenuNamecommentWBC「ItemsWEC3PLT聚集手工計數(shù)175h~i二opyPLTAbnDStR.esearch(w)|Research(R^i||curnu1ative|q-f1ags|service|hpc■WBCQ-ifferentialRETRBCPLTZ5OFLWBC/BASOb<DIFFItemDataUnitMEUT%81.2+%LYMPH%14,0-%MONO%3.9%EQ%0.6%BAS。尨0.3%ItemDataUnitWBCRBCHGBHCTMCVMCHMCHCPLT6.204.6013641.991.129.632575ifir10A9/L10A12/Lg/L%fLpgg/L10A9/LRDW-SDRDW-CVPDWMPVP-LCRPCT46.013.9fL%fL什%%RET%RET#IRiFLFRMFRHFR%10A12/L%%%%NRBC#NREC%10A9/L/100WECSampleNo.f8251Birth廠Ward[Date12009/05/12Sexpat.id

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