不同來源間充質干細胞標志物匯總（全）

不同來源間充質干細胞標志物匯總(全)(圖片來源網(wǎng)絡)間充質干細胞(mesenchymalstemcells,MSC)是一種源于中胚層的早期干細胞，具有多向分化潛能，特定的條件下可分化為骨細胞、軟骨細胞和神經(jīng)細胞等，支持造血，具備低免疫原性和免疫調節(jié)活性，具有廣泛的科研和臨床應用價值。主要存在于結締組織和器官間質中，以骨髓中含量最為豐富，少量存在于血液及其他組織中。近年來隨著基礎科研和臨床研究的不斷深入，MSC在醫(yī)學領域的應用價值也得到了極大肯定，但仍然存在一些問題，如：缺少特異性表面標志物，鑒定困難；多次傳代后分化能力不能保留；誘導分化調控機制尚不明確等，都是有待于進一步研究和討論的。MSC主要關鍵基因和表面分子包括CD73、CD90、CD105，表達極少或無表達CD11b、CD19、CD34、CD45和HLA-DR，其中不同來源之間的MSC標志物存有一定差異性，這里列出來供大家參考。外周血(Peripheralderivedblood)來源[1]陽性:CD105,CD90,CD73,CD73,CD44,CD90.1,CD29,CD105,CD106,CD140a陰性：CD34,CD19,CD11b骨髓(Bonemarrow)來源[2]陽性：CD105,CD13,CD140b,CD147,CD151,CD276,CD29,CD44,CD47,CD59,CD73,CD81,CD90,CD98陰性：CD14,CD31,CD34,CD45皮膚(Skin)/包皮(foreskin)來源[3]陽性：CD29,CD44,CD73,CD90,CD105,vimentin陰性：CD34,CD45,HLADR脂肪(Adipose)來源[4]陽性：CD9,CD29,CD44,CD54,CD73,CD90,CD105,CD106,CD146,CD166陰性：CD14,CD31,CD34,CD45,CD133,CD144,HLA-DR,STRO-1尿液（Urine）來源[5,6]陽性：CD29,CD44,CD54,CD73,CD90,CD105,CD166,STRO-Oct-4,Klf-4,Sox-17,vimentin陰性：CD41,HLA-DR心（Heart）來源[7]陽性：CD44,CD105,CD29,CD90陰性：CD14,CD45,CD34,CD31牙（Dental）來源[8,9]陽性：CD13,CD29,CD44,CD49,CD73,CD90,CD146,STRO-1,Oct-3/4,NANOG,SSEA-3陰性：CD14,CD31,CD34,CD45,HLADR骨骼?。⊿keletalmuscle）來源[10]陽性：CD29,CD44,CD49E,CD56,CD73,CD90,CD105,HLA-I陰性：CD34,CD45胰腺（Pancreas）來源[11]陽性：CD105,CD90,CD73,CD44,CD29,CD13,nestin,vimentin,CD146,NG2,a-SMA,PDGF-R[3陰性：CD31,CD34,andCD45,CK19,CA19.9肺（Lung）來源[12]陽性：CD73,CD90,andCD105,vimentin,prolyl-4-hydroxylase陰性：CD14,CD34,CD45胚胎干細胞（ESC）[13]陽性：CD29,CD44,CD73,CD105,SSEA-4陰性：CD34,CD45,HLADR誘導多能干細胞（iPSC）[14]陽性：CD29,CD44,CD166,CD73,CD105,KDR,MSX2陰性：CD34,CD45,HLADR沃頓果凍(Whartonjelly)來源[15]陽性：CD44,CD73,CD90,CD105,CD166陰性：CD14,CD34,CD45胎盤(Placenta)來源[16,17]陽性：CD105, CD73,CD90c-kit,Thy-1,Oct-4,SOX2,hTERT,SSEA-1,3,4,TRA-1陰性：CD34,CD45,CD14orCD11b,CD19,HLA-DR臍帶(Umbilicalcord)來源[18]陽性:CD73,CD90,CD105,Oct-4,Nanog,ABCG2,Sox-2,Nestin陰性：CD34,CD45,CD19,HLA-DR絨毛(Chorionicvilli)來源[19]陽性：CD44,CD117,CD105,a-SMA,CD49,CD146,CD106,CD166,Stro-1,vWF陰性：CD34,CD45,CD19,HLA-DR絨毛膜(Chorionicmembrane)來源[20]陽性：CD44,CD49,CD56,CD73,CD90,CD105陰性：CD45,CD34,CD14,CD31,EPCAM,HLA-DR臍帶血(Cordblood)來源[17]陽性：CD29,CD73,CD105,CD44,Oct-4,Sox-1,Sox2,NANOG,ABCG2,Nestin陰性：CD34,CD45子宮內膜(子宮內膜)來源[21]陽性：CD73,CD90,CD105,CD166,HLA-ABC,Oct-4陰性：CD14,CD34,CD45,HLA-DR羊膜(Amnioticmembrane)來源[22,23]陽性：CD73,CD90,CD105,Oct-4,SSEA-4,Tra-1陰性：CD11b,CD14,CD19,CD79a,CD34,CD45,HLA-DR羊水(Amnioticfluid)來源[24]陽性：CD73,CD90,CD105,CD166,MHCclassI,Oct-4,EA-1陰性：CD45,CD40,CD34,CD14,HLA-DR參考文獻：LiS,HuangK-J,WuJ-C,HuMS,SanyalM,HuM,LongakerMT,LorenzHPPeripheralblood-derivedmesenchymalstemcells:candidatecellsresponsibleforhealingcritical-sizedcalvarialbonedefects.StemCellsTranslMed.2015;4(4):359-68.AmatiE,PerbelliniO,RottaG,BernardiM,ChieregatoK,SellaS,RodeghieroF,RuggeriM,AstoriG.High-throughputimmunophenotypiccharacterizationofbonemarrow-andcordblood-derivedmesenchymalstromalcellsrevealscommonanddifferentiallyexpressedmarkers:identificationofangiotensinconvertingenzyme(CD143)asamarkerdifferentiallyexpressedbetweenadultandperinataltissuesources.StemCellResTher.2018;9(1):10.ParkJR,KimE,YangJ,LeeH,HongSH,WooHM,ParkSM,NaS,YangSR.Isolationofhumandermisderivedmesenchymalstemcellsusingexplantsculturemethod:expansionandphenotypicalcharacterization.CellTissueBank.2015;16(2):209-18.SchafflerA,BuchlerC.Concisereview:adiposetissue-derivedstromalcells—basicandclinicalimplicationsfornovelcell-basedtherapies.StemCells.2007;25(4):818-27.ZhangD,WeiG,LiPZhouX,ZhangY.Urine-derivedstemcells:anovelandversatileprogenitorsourceforcell-basedtherapyandregenerativemedicine.GenesDis.2014;1(1):8-17.PavathuparambilAbdulManaphN,Al-HawwasM,BobrovskayaL,CoatesPT,ZhouX-F.Urine-derivedcellsforhumancelltherapy.StemCellResTher.2018;9(1):189.PilatoCA,StadiottiI,MaioneAS,SaverioV,CattoV,TundoF,DelloRussoA,TondoC,PompilioG,CasellaM,etal.Isolationandcharacterizationofcardiacmesenchymalstromalcellsfromendomyocardialbiopticsamplesofarrhythmogeniccardiomyopathypatients.JVisExp.2018;132:57263.HuangGTJ,GronthosS,ShiS.Mesenchymalstemcellsderivedfromdentaltissuesvs.thosefromothersources:theirbiologyandroleinregenerativemedicine.JDentRes.2009;88(9):792-806.Ledesma-MartinezE,Mendoza-NunezVM,Santiago-OsorioE.Mesenchymalstemcellsderivedfromdentalpulp:areview.StemCellsInt.2016;2016:4709572.CamernikK,MihelicA,MihalicR,MaroltPresenD,JanezA,TrebseR,MarcJ,ZupanJ.Skeletal-muscle-derivedmesenchymalstem/stromalcellsfrompatientswithosteoarthritisshowsuperiorbiologicalpropertiescomparedtobone-derivedcells.StemCellRes.2019;38:101465.MAhmedS,El-BadriN.Pancreaticmesenchymalstromalcells:characteristicsandpossibleorigin.LiverPancreaticSci.2016;1(1).MartinuT,PalmerSM,OrtizLA.Lung-residentmesenchymalstromalcells.AmJRespirCritCareMed.2011;183(8):968-70.HawkinsKE,CorcelliM,DowdingK,RanzoniAM,VlahovaF,HauKL,HunjanA,PeeblesD,GressensF,HagbergH,etal.Embryonicstemcell-derivedmesenchymalstemcells(MSCs)haveasuperiorneuroprotectivecapacityoverfetalMSCsinthehypoxic-ischemicmousebrain.StemCellsTranslMed.2018;7(5):439-49.XuM,ShawG,MurphyM,BarryF.Inducedpluripotentstemcellderivedmesenchymalstromalcellsarefunctionallyandgeneticallydifferentfrombonemarrow-derivedmesenchymalstromalcells.StemCells.2019;37(6):754-65.PittengerMF,MackayAM,BeckSC,JaiswalRK,DouglasR,MoscaJD,MoormanMA,SimonettiDW,CraigS,MarshakDR.Multilineagepotentialofadulthumanmesenchymalstemcells.Science.1999;284(5411):143-7.MatikainenT,LaineJ.Placenta--analternativesourceofstemcells.ToxicolApplPharmacol.2005;207(2Suppl):544-9.delaTorreP,JesusPerez-LorenzoMI,FloresA.Humanplacenta-derivedmesenchymalstromalcells:areviewfrombasicresearchtoclinicalapplications;2019.IwataniS,YoshidaM,YamanaK,KurokawaD,KurodaJ,ThwinKKM,UemuraS,TakafujiS,NinoN,KodaT,etal.Isolationandcharacterizationofhumanumbilicalcord-derivedmesenchymalstemcellsfrompretermandterminfants.JVisExp.2019;143.DivyaMS,RoshinGE,DivyaTS,RasheedVA,SanthoshkumarTR,ElizabethKE,JamesJ,PillaiRM.Umbilicalcordblood-derivedmesenchymalstemcellsconsistofauniquepopulationofprogenitorsco-expressingmesenchymalstemcellandneuronalmarkerscapableofinstantaneousneuronaldifferentiation.StemCellResTher.2012;3(6):57.GonzalezPL,CarvajalC,CuencaJ,Alcayaga-MirandaF,FigueroaFE,BartolucciJ,Salazar-AravenaL,KhouryM.Chorionmesenchymalstemcellsshowsuperiordifferentiation,immunosuppressive,andangiogenicpotentialsincomparisonwithhaploidenticalmaternalplacentalcells.StemCellsTranslMed.2015;4(10):1109-21.ChengY,LiL,