進(jìn)出口糧谷中玉米赤霉烯酮的測(cè)定 免疫親合柱-液相色譜法

進(jìn)出口糧谷中玉米赤霉烯酮的測(cè)定免疫親和柱-液相色譜法b)流動(dòng)相：乙腈+水+甲醇(46+46+8); —添加濃度在0.050mg/kg時(shí)，回收率為76.5%～90.3%;—滯加濃度在0.500mg/kg時(shí)，回收率為87.6%～98.2%。4.2.2小麥中玉米赤霹烯酮的添加濃度及回收率實(shí)驗(yàn)數(shù)據(jù)；—深加濃度在0.005mg/kg時(shí)，國(guó)收率為76.89%~90.7%;—深加濃度在0.050mg/kg時(shí)，回收率為78.3%～93.6%;—深加濃度在0.500mg/kg時(shí)，回收率為86,4%～93.2%。ThisstandardspeciflesthemethodofsamplumnandliquldchromatographyofzearalenoneinceThisstancardisapplicableforthedeterminationofzearalenonein2.1preparationoftestsieve,mixthoroughlyanddivideintotwooqualportions,Duringsam-plingandpreparationofsample,panyfactorsthatmaycausethechangeofThetestsampleshallbes3MethodofdetThezearalenoneinthetestsampleareextractedwithaimmunoaffinitycolumnforcleanup.Determinationismadeaquippedwithfluorescencedetector,usingexternalstaUnlessotherwisespecified,allreagentsshouldbeofanalyticalgradcorrespondingde-ionizedwater.3.2.2Acetonitrile;HPL3.2.3Acetonitrile-water(9+1):(90+10,V3.2.5Zearalenonestandard:Purity≥98%.6Zoaralenonestandardstoredsolution;Accuratelywoighfzoaralenonestandard,dissolveinacetonitrillutethestandardstocksolutionwithmobileph3.3.1Highperformanceliquidchromatographequippedwith3.3.8Immunoaffinitycolumn:ZearalenoneiWeigh40g(accurateto0.1g)ofthetes100mLacetonitrilewater(90+10,V/V)bybwasfilteredthroughfilterpaper,10.0mLoft 9X—theresiduecontentofzearalenoneinthetestsamA—thepeakareaofzearalemoneinthesaA?—thepeakareaofzearalemoneintc—theconcentrationofzearalenoneinthestandardworkingsoluV—thefinalvolumeofthesamplesm—thecorrespondingmassofthetestsampleinthe4LimitofdetermiThelimitofdeterminat4.2.1Accordingtoexperimentaldata,thefortifyingcorrespondingreco—0.005mg/kg,therecover—0.050mg/kg,there—0.500