標(biāo)準(zhǔn)解讀
《GB/T 44611-2024 栽培介質(zhì)有害生物檢測規(guī)程》是一項(xiàng)國家標(biāo)準(zhǔn),旨在規(guī)范栽培介質(zhì)中可能存在的有害生物的檢測方法與流程。該標(biāo)準(zhǔn)適用于農(nóng)業(yè)、園藝等領(lǐng)域內(nèi)使用的各種栽培介質(zhì)(如土壤、泥炭、椰糠等)中有害生物(包括但不限于昆蟲、螨類、線蟲、真菌、細(xì)菌和病毒)的檢測。
標(biāo)準(zhǔn)首先明確了適用范圍及術(shù)語定義,確保使用者能夠準(zhǔn)確理解其內(nèi)容并正確應(yīng)用。接著,詳細(xì)描述了樣品采集的方法,強(qiáng)調(diào)了采集過程中需要注意的關(guān)鍵點(diǎn),比如避免交叉污染、保持樣品完整性等原則,以保證后續(xù)檢測結(jié)果的有效性和準(zhǔn)確性。
在樣品處理部分,《GB/T 44611-2024》提供了多種處理技術(shù)的選擇指南,根據(jù)不同的目標(biāo)生物類型推薦相應(yīng)的預(yù)處理步驟,如篩選、漂浮分離或化學(xué)提取等,以便于更有效地分離出待檢生物體。
對于具體的檢測方法,《GB/T 44611-2024》列舉了幾種常見且有效的技術(shù)手段,包括顯微鏡觀察法、分子生物學(xué)鑒定法(如PCR)、免疫學(xué)測試(ELISA)以及培養(yǎng)基直接接種法等,并對每種方法的操作細(xì)節(jié)進(jìn)行了說明。此外,還特別指出了某些特定情況下應(yīng)采取的特殊措施,比如針對難以培養(yǎng)或生長緩慢的微生物時(shí)可采用的替代方案。
如需獲取更多詳盡信息,請直接參考下方經(jīng)官方授權(quán)發(fā)布的權(quán)威標(biāo)準(zhǔn)文檔。
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- 即將實(shí)施
- 暫未開始實(shí)施
- 2024-09-29 頒布
- 2025-04-01 實(shí)施
文檔簡介
ICS
65.020.20
CCS
B16
中華人民共和國國家標(biāo)準(zhǔn)
GB/T44611—2024
栽培介質(zhì)有害生物檢測規(guī)程
Codeofpracticeforthedetectionofpestsinthegrowingmedium
2024-09-29發(fā)布2025-04-01實(shí)施
國家市場監(jiān)督管理總局發(fā)布
國家標(biāo)準(zhǔn)化管理委員會(huì)
GB/T44611—2024
目次
前言
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Ⅲ
1
范圍
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1
2
規(guī)范性引用文件
······················································································
1
3
術(shù)語和定義
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1
4
栽培介質(zhì)基本信息
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1
5
檢測程序
······························································································
1
6
檢測對象及檢測依據(jù)
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2
6.1
檢測對象
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2
6.2
檢測依據(jù)
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2
7
現(xiàn)場檢驗(yàn)
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3
8
實(shí)驗(yàn)室檢測
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3
8.1
昆蟲及軟體動(dòng)物檢測
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3
8.2
線蟲檢測
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3
8.3
植物病原菌物檢測
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3
8.4
植物病原細(xì)菌檢測
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3
8.5
雜草檢測
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4
9
檢測結(jié)果報(bào)告
·························································································
4
10
綜合判定
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4
11
檢測記錄及樣品保存
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4
11.1
檢測記錄內(nèi)容
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4
11.2
樣品及記錄保存
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4
附錄A(資料性)
栽培介質(zhì)中主要檢疫性有害生物名單
·············································
6
A.1
線蟲
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6
A.2
菌物
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6
A.3
細(xì)菌
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7
附錄B(規(guī)范性)
線蟲分離方法
······································································
8
B.1
漂浮分離法
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8
B.2
改良漏斗法
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8
附錄C(規(guī)范性)
菌物檢測方法
······································································
9
C.1
洗滌檢驗(yàn)法
······················································································
9
C.2
稀釋分離法
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9
C.3
選擇性培養(yǎng)基分離法
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9
參考文獻(xiàn)
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10
Ⅰ
GB/T44611—2024
前言
本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分:標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)
定起草。
請注意本文件的某些內(nèi)容可能涉及專利。本文件的發(fā)布機(jī)構(gòu)不承擔(dān)識別專利的責(zé)任。
本文件由全國植物檢疫標(biāo)準(zhǔn)化技術(shù)委員會(huì)(SAC/TC271)提出并歸口。
本文件起草單位:中國檢驗(yàn)檢疫科學(xué)研究院、寧波海關(guān)技術(shù)中心、蘇州晶睿生物科技有限公司、
滿洲里海關(guān)、寧夏回族自治區(qū)農(nóng)業(yè)技術(shù)推廣總站、福州海關(guān)技術(shù)中心、山東大學(xué)。
本文件主要起草人:田茜、顧建鋒、段維軍、吳堯、劉瑋琦、李秋實(shí)、徐晗、杜偉、于文濤、
張偉。
Ⅲ
GB/T44611—2024
栽培介質(zhì)有害生物檢測規(guī)程
1范圍
本文件給出了栽培介質(zhì)的基本信息,確立了栽培介質(zhì)有害生物檢測程序,規(guī)定了檢測對象的確定及
檢測依據(jù)的選擇、現(xiàn)場檢驗(yàn)、實(shí)驗(yàn)室檢測、出具檢測結(jié)果報(bào)告以及綜合判定的過程,描述了記錄及保存
的證實(shí)方法。
本文件適用于進(jìn)出境及國內(nèi)生產(chǎn)流通的栽培介質(zhì)中有害生物的檢測鑒定。
2規(guī)范性引用文件
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件,僅該日
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