L-Cystine-hydrochloride-生命科學(xué)試劑-MCE

Hotline:400-820-3792Inhibitors ? ScreeningLibraries ? Proteinswww.MedChemEL-CystinehydrochlorideCat.No.:HY-W009356CASNo.:34760-60-6分子式:C?H??Cl?N?O?S?分子量:313.22作用靶點(diǎn):EndogenousMetabolite;Ferroptosis;ROSKinase;Keap1-Nrf2;ReactiveOxygenSpecies作用通路:MetabolicEnzyme/Protease;Apoptosis;ProteinTyrosineKinase/RTK;NF-κB;Immunology/Inflammation儲(chǔ)存方式:RT,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(RT,protectfromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)H2O:125mg/mL(399.08mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM3.1926mL15.9632mL31.9264mL5mM0.6385mL3.1926mL6.3853mL10mM0.3193mL1.5963mL3.1926mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存方式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。BIOLOGICALACTIVITY生物活性L-Cystinehydrochloride是具有口服活性的L-Cysteine(HY-Y0337)的細(xì)胞外形式，存在于植物和動(dòng)物的蛋白質(zhì)中。L-Cystinehydrochloride升高Nrf2蛋白表達(dá)含量并激活Nrf2轉(zhuǎn)錄因子。L-Cystinehydrochloride減少了ROS的產(chǎn)生，并保護(hù)細(xì)胞免受氧化劑或Doxorubicin(HY-15142A)誘導(dǎo)的凋亡(apoptosis)。L-Cystinehydrochloride與L-theanine(HY-15121)聯(lián)合使用通過提高谷胱甘肽(GSH)水平和T輔助細(xì)胞2(Th2)介導(dǎo)的反應(yīng)，增強(qiáng)了抗原特異性IgG的產(chǎn)生。L-Cystinehydrochloride有望用于囊性尿癥和腎結(jié)石的研究[1][2][3]1/3 MasterofBioactiveMolecules—您身邊的抑制劑大師www.MedChemE[4][5][6]。體外研究L-Cystinehydrochloride(83μMor200μM,24hor72h)effectivelyreversesthegrowthinhibitionofbothSulfasalazine(HY-14655)andErastin(HY-15763)inHuh6cells[1].L-Cystinehydrochloride(0.8mM,4h)inducesNrf2proteinexpressionelevationinaKeap1-dependentmannerinHeLacells[6].CellViabilityAssay[1]CellLine:Huh6andHuh7cellsConcentration:83μMor200μMIncubationTime:72hor24hResult:ThedegreeofgrowthinhibitionbySulfasalazine(HY-14655)orErastin(HY-15763)wasgreaterinmediumcontainingaphysiologicallevelof83μML-Cystinehydrochloridethaninmediumcontaining200μML-CystinehydrochlorideinHuh6andHuh7cells.WesternBlotAnalysis[6]CellLine:HeLacellsConcentration:0.2mMIncubationTime:4hResult:WassufficientforproducingNrf2proteinelevationat0.2mMinHeLacells.WesternBlotAnalysis[6]CellLine:HeLacellsConcentration:0.8mMIncubationTime:4hResult:InducedNrf2inaKeap1-dependentmannerinHeLacells.體內(nèi)研究L-Cystinehydrochloride(280mg/kg,p.o.,11days)administratedwithL-theanine(HY-15121)enhancesantigen-specificIgGproductionpartlythroughaugmentationofglutathione(GSH)levelsandThelper2(Th2)-mediatedresponsesinmice[2].L-Cystinehydrochloride(200mg/kg,p.o.,11days)administratedwithL-theanine(HY-15121)(80mg/kg,p.o.,11days)significantlydecreasestheserumIL-10/IFN-γratio6hoursafterDNP-KLHantigenicstimulation,butincreasestheserumIL-10/IFN-γratio24hoursafterDNP-KLHantigenicstimulationinmice[2].AnimalModel:Nine-week-oldfemaleBALB/cmice[2]Dosage:140,280and560mg/kg2/3 MasterofBioactiveMolecules—您身邊的抑制劑大師www.MedChemEAdministration: p.o.administrationfor11daysResult: SignificantlyincreasedIgGandIgMantibodyproductionadministratedwithL-theanine(HY-15121)afterDNP-dextranandDNP-KLHantigenicstimulationinmice.REFERENCESAbdullahM,etal.ExtracellularConcentrationofL-CystineDeterminestheSensitivitytoSystemxc-Inhibitors[J].BiomolTher(Seoul).2022Mar1;30(2):184-190.KuriharaS,etal.Enhancementofantigen-specificimmunoglobulinGproductioninmicebyco-administrationofL-cystineandL-theanine[J].JVetMedSci.2007Dec;69(12):1263-70.DominiqueBazin,etal.CystinuriaandcystinosisareusuallyrelatedtoL-cystine:isthisreallythecaseforcystinosis?Aphysicochemicalinvestigationatmicrometreandnanometrescale.ComptesRendus.Chimie,Volume25(2022)no.S1,pp.489-502.GrethWE,etal.CellularaccumulationofL-cystineinratkidneycortexinvivo[J].JClinInvest.1973Feb;52(2):454-62.OhtsuI,etal.UptakeofL-cystineviaanABCtransportercontributesdefenseofoxidativestressintheL-cystineexport-dependentmannerinEscherichiacoli.PLoSOne.2015Apr2;10(3):e0120619.DaiW,etal.FreshMediumorL-CystineasanEffectiveNrf2InducerforCytoprotectioninCellCulture[J].Cells.2023Jan12;12(2):291.M