DNA試驗(yàn)-免疫細(xì)胞表面抗原分子CD家族對(duì)照表CDCD24

1、免疫細(xì)胞表面抗原分子CD家族對(duì)照表(CD1-CD247)ExalphaBiologicalsproducesandsellsproductsforLifeScienceResearch,includingmonoclonalandpolyclonalantibodies,foruseinimmunohistochemistry,westernblot,ELISAandFlowCytometry.Weofferover800productsand,throughcollaborationswithotherscientistsaswellasourownin-houseR&Dprograms,E

2、xalphacontinuestobringleadingedgeproductstomarket.OurfamilyofCDantibodiescombinesqualityandperformancewithreasonablepricestomakeourCDantibodiesthebestvalueinthemarkettoday.OurfamilyofProliferationproductsoffereasyall-in-onekitsorstandaloneantibodiestoquantitateproliferatingcells.Takealookatourwebsit

3、eorcallusforadditionalinformation.Note:noinformationisavailableonCDw145,CD181,CD182,CD185-194,CD196-199,CD211,CD214-216,CD218-220,CD237BackgroundCD:clusterdesignationofmonoclonalantibodies(clustersofdifferentiation)Designatedatthe1stto7thWorkshopsonInternationalHumanLeukocyteDifferentiationAntigensT

4、helastconferencewasin2000.Thenextconferenceisin2004.HLDAWorkshopsaretheprimarymechanismtocharacterizeleukocytesurfaceantigenicmoleculesandepies;erythroidantigensarenowincludedFor1stto6thworkshops,antibodiesweresubmittedtotheorganizinglaboratory,codedandsenttoparticipatinglaboratoriesfortestingagains

5、tvariouscelltypes.Forthe7thworkshop,aCDdesignationcouldbeestablishedforamoleculeifitsgenehasbeenclonedandatleastonespecificmonoclonalantibodyhadbeenstudiedintheWorkshopInterpretationshouldbebasedoncellulardistributionofstaining,proportionofpositivelystainedcells,stainingintensityandcutofflevels.CD1F

6、amilyofnon-polymorphicMHCclassI-likeglycoproteinsAlsomemberofimmunoglobulinsuperfamilyOnchromosome1q22-23(notMHClinked)Has5differentsubsets,allnoncovalentlyassociatedwith12kdbeta2microglobulinFunction:restrictTcellresponsestocertainantigens;maymediatethymicTcelldevelopmentPositivestaining(normal):co

7、rticalthymocytes(70%),activatedTcells,Langerhanscells,interdigitatingdendriticcellsPositivestaining(disease):preTALLwithcorticalthymocytephenotype;LangerhanscellhistiocytosisNegativestaining:matureperipheralTcellsCD1aPositivestaining(normal):Dendriticcellsindermis/epidermisofbenigninflammatoryskindi

8、sordersPositivestaining(disease):Langerhanscellhistiocytosis(fairlyspecific),myeloidleukemias,someBcellmalignancies;dendriticcellsinmostperipheralcutaneousTcelllymphomas,AJCP2001;116:72Negativestaining:normalBcells,mostcutaneousperipheralBcelllymphomas(?reflectsreplacementofreactivepatterncontaining

9、dendriticcellswithaneoplasticpatternofBcells)Microimages:LangerhanscellhistiocytosisMicroimages(AJSPsubscribers):pulmonaryLangerhanscellhistiocytosisMicroimages(HumPathsubscribers):LangerhanscellhistiocytosisReferences:AJSP2001;25:630CD1bPositivestaining(disease):myeloidleukemiasandsomeBcellmalignan

10、ciesNegativestaining:normalBcellsCD1cPositivestaining(normal):subsetofnormalperipheralBcellsPositivestaining(disease):myeloidleukemiasandsomeBcellmalignanciesNegativestaining:normalBcellsCD1dPositivestaining(normal):thymus(lowlevels),bowelCD1eCD2AkaErosettereceptor,LFA-2(leukocytefunctionantigen)Fun

11、ction:bindsCD58/LFA-3onantigen-presentingcells,andinducescostimulatorysignalsinTcellsAlsoregulatesTandNK-mediatedcytolysis,inhibitsapoptosisofactivatedperipheralTcells,mediatesTcellcytokineproduction,regulatesTcellanergyPositivestaining(normal):thymocytes(95%),matureperipheralTcells(almostall),NKcel

12、ls(80-90%),thymicBcells(50%)Microimages:extranodalNK/Tcelllymphoma,nasaltypeCD2RCD2epiesrestrictedtoactivatedTcellsPositivestaining:activatedTcells,?NKcellsCD3AkaOKT3Function:complex(5chains)ofintegralmembraneglycoproteinsassembledasacomplex;haslongcylasmictailwithantigenrecognitionactivationmotif;c

13、omplexisthendownregulatedAlsosubdividedintodelta,epsilon,gammasubtypesCylasmicexpressionatearlyTcelldifferentiation,thenmembranousexpressionMostspecificTcellantibodyPositivestaining(normal):thymocytes,peripheralTcells,NKcells;alsoPurkinjecellsofcerebellumPositivestaining(disease):80%ofTcelllymphomas

14、Negativestaining:gammadeltaTcellreceptors,mostBcelllymphomasMicroimages:CD3epsilon-testicularNK/Tcelllymphoma(figure3D)achalasia,post-transplantlymphoproliferativeMicroimages(AJSPsubscribers):diseaseinliverReferences:AJSP2001;25:1413CD4AkaOKT4,Thelper/inducerOnchromosome#12pNonpolymorphousglycoprote

15、insbelongingtoimmunoglobulinsuperfamilyServesasHIVreceptoronTcells(asdochemokinereceptorsCCR5andCXCR4),macrophages,brainCD4+TcellsarekilledbyHIVCoreceptorinMHCclassII-restrictedantigeninducedTcellactivationBindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcell-cellinteractionsPositive

16、staining(normal):thymocytes(80-90%),matureTcells(65%,ThelperandpityriasislichenoidesCD4/CD8+thymocytes),macrophages,Langerhanscells,dendriticcells,granulocytesPositivestaining(disease):Microimages:acutedemyelinatingdisease,extranodalNK/Tcelllymphoma,nasaltypeCD5Belongstoancientscavengerreceptorfamil

17、yIsphysicallyandfunctionallycoupledwithTcellreceptor-zeta-CD3signaltransducercomplexCD5+Bcellsproduce“generalistantibodies”-polyreactivelowaffinity“naturalantibodiestoexogenousantigens(tetanustoxoid,lipopolysaccharide)aswellasautoreactiveantibodies(ssDNA,thyroglobulin,insulin)Note:sharksonlyhavepoly

18、reactiveIgMNote:monoreactiveIgGisproducedby0.1%ofcirculatingBcells,frompositiveselectionandsomaticpointmutationFirstlineofdefenseagainstantigens;havealowactivationthreshold;aretheonlylineofdefenseforthosewhocannotproducespecificantibodyProduceantibodiesusinggermline(nonmutated)configurationofgeneseg

19、ments,usuallyIgMProductionelevatedinrheumatoidarthritis(27-52%ofcirculatingBcellsvs.20%normal)CD5mayserveasadualreceptor,givingeitherstimulatoryorinhibitorysignalsdependingbothonthecelltypeandthedevelopmentstagePositivestaining(normal):Bcellsofmantlezoneofspleenandlymphnodes;Bcellsinperitonealandple

20、uralcavities;almostallTcells;Infetus,mostBcellsinspleenandcordbloodareCD5positivePositivestaining(disease):BcellCLL/SLL,mantlecelllymphoma,mostTmalignancies,thymiccarcinomas(70%)Negativestaining:spindlecellthymomas,MALTlymphoma,follicularlymphomaMicroimages:extranodalNK/Tcelllymphoma,nasaltype,mantl

21、ecelllymphoma(figure3D)CD6AdhesionmoleculemediatingthebindingofdevelopingthymocyteswiththymicepithelialcellsMaybeinvolvedinautoimmunityandgraftvs.hostdisease(GVHD)AntibodiestoCD6areusedtodepleteTcellsfrombonemarrowtransplantstopreventPositivestaining(normal):thymocytesGVHDlowlevelsonimmaturethymocyt

22、es,highlevelsonmatureCD7MembraneglycoproteinandFcreceptorforIgMHomologoustoTCRgamma,IgkappaMembraneexpressionearlyduringTontogeny,beforeTCRrearrangement,persistsuntilterminalstagesofTcelldevelopmentLowerexpressioninmemoryTcellsvs.naiveTcellsPositivestaining(normal):matureperipheralTcells(85%),post-t

23、hymicTcells(majority),NKcells(majority),somemyeloidcellsPositivestaining(disease):TcellALL;AML(especiallyM4/M5),chronicmyelogenousleukemia,blastsintransientmyeloproliferativedisorderNegativeexpression:BcellALL,Sezarysyndrome,adultTcellleukemia/lymphomaMicroimages:extranodalNK/Tcelllymphoma,nasaltype

24、CD8AkaOKT8,Tcellsuppressor/cytotoxiccellsOnchromosome#2MHCclassIrestrictedreceptor;bindstononpolymorphicregionofclassImolecules;mayincreaseavidityofcell-cellinteractionsAssociatedwithlymphoepithelioma-likecarcinomaoflung(AJSP2002;26:715Positivestaining(normal):Tcells(25-35%ofmatureperipheralTcells,m

25、ostcytotoxicTcells,CD4/CD8+thymocytes);NKcells(30%-whicharealsoCD3negative);corticalthymocytes(70-80%),epidermotrophiclymphocytesinmycosisfungoides(AJSP2002;26:450)Microimages:lymphoepithelioma-likecarcinomaofcervix-figure3,acutedemyelinatingdiseaseMicroimages(ModPathsubscribers):nodalcytotoxicTcelllymphomaReference:ModPath2002;15:1131CD9MaymediateplateletactivationandaggregationAntibodiesareusedtopurgebonemarrowpriortoperipheralstemcellbonemarrowtransplantViralco-receptorPositivestaining(normal):preB