黃芩苷作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetBaicalinCat. No.: HY-N0197CAS No.: 21967-41-9分式: CHO分量: 446.36作靶點(diǎn): NF-B; Autophagy; HIV作通路: NF-B; Autophagy; Anti-infection儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (224.03 mM)H2O : 0.1 mg/mL (insoluble)* means soluble, but sat

2、uration unknown.SolventMass1 mg 5 mg 10 mgConcentration制備儲(chǔ)備液1 mM 2.2403 mL 11.2017 mL 22.4034 mL5 mM 0.4481 mL 2.2403 mL 4.4807 mL10 mM 0.2240 mL 1.1202 mL 2.2403 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)內(nèi)使，-20C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)內(nèi)使。體內(nèi)實(shí)驗(yàn)

3、請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍浮Ｒ韵氯芙獍付颊?qǐng)先按照 In Vitro 式配制澄清的儲(chǔ)備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄 的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的式助溶1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.60 mM); Clear solution此案可獲得 2.5 mg/mL (5.60 mM，

4、飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲(chǔ)備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (5.60 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 2.5 mg/mL (5.60 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 10

5、0 L 25.0 mg/mL 的澄 DMSO 儲(chǔ)備液加到 900 L 20% 的 SBE-CD 理鹽溶液中，混合均勻。BIOLOGICAL ACTIVITY物活性 Baicalin種從 Scutellaria baicalensis 中分離出來(lái)的類(lèi)黃酮糖苷。Baicalin 降低 NF-B 表達(dá)。IC & Target NF-B Autophagy體外研究 Baicalin protects against ischemia-reperfusion injury (IRI) by altering the production of various mediators, includingre

6、active oxygen species (ROS), Toll-like receptor (TLR)2 and TLR4, NF-B, Bax, and Bcl-2. Baicalin treatment inhibitsthe increased expression of the proinflammatory cytokines TLR2/4, MyD88, p-NF-B, and p- IB, as well as increasethe expression of IB protein, an NF-B inhibitor, with the degree of inhibit

7、ion positively related to the dosage ofBaicalin1. Cell viability is determined by MTT assay. Compared with control cells, cell viability is significantlydecreased in SH-SY5Y cells treated with thrombin. Pre-treatment with Baicalin (5, 10, 20 M) increases cell viability ina dose-dependent manner comp

8、ared with cells treated thrombin alone2.體內(nèi)研究 Baicalin pretreatment dose-dependently protects against a loss of renal function, with the two higher doses (10 and100 mg/kg) significantly decreasing Scr and blood urea nitrogen (BUN) concentrations. Tissue injury, as assessed using a 0-3 point scoring s

9、ystem, is lower for the Baicalin treated groups than for the ischemia-reperfusion (IR)+salinegroup. Compared with the sham group, malondialdehyde (MDA) content is only slightly up-regulated and the SODactivity is only slightly down-regulated in rats treated with 10 and 100 mg/kg Baicalin, indicating

10、 that Baicalinabrogates the increase in oxidative stress following reperfusion1.PROTOCOLCell Assay 2 SH-SY5Y cell lines are cultured in RPMI-1640 medium supplemented with 15% fetal bovine serum at 37C in an airatmosphere containing 95% air and 5% CO2 with a saturated humidity. Upon a confluence of 6

11、070%, the SH-SY5Ycells are divided into: (i) control group, incubated in RPMI-1640 medium; (ii) thrombin group, which is subject tothrombin induction (40 U/L) for 6 h based on our pre-experiment; and (iii) Baicalin groups, which are treated byBaicalin (5 M, 10 M, or 20 M) for 2 h before induction of

12、 thrombin. Cell viability is measured using MTT assay.Briefly, 15 L of the MTT solution (5 mg/mL) is added to each well and incubated for 4 h at 37C. After removing thesupernatant, 80 L DMSO are added into each well. The absorbance is measured at 492 nm using a microplatereader. All experiments are

13、performed in triplicate2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Rats1Administration 1 Male Wistar rats weighing 200-250 g are used. Rats are randomly divided into five groups of six rats each: (i) sham group; (ii) IR+saline group; (iii)

14、IR+Baicalin (1 mg/kg) group; (iv) IR+Baicalin (10 mg/kg) group; and (v) IR+Baicalin(100 mg/kg) group. Renal IRI is induced by clamping the left renal artery for 45 min plus a right nephrectomy. Ratsare anesthetized through an intraperitoneal injection of pentobarbital sodium (40 mg/kg body weight).

15、After amedian abdominal incision, the left renal arteries are clamped for 45 min with serrefine. After clamp removal,adequate restoration of blood flow is checked before abdominal closure. The right kidney is then removed. Sham-operated animals underwent the same surgical procedure without clamping1

16、.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Page 2 of 3 www.MedChemE戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Oral Dis. 2019 Aug 8.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Lin M, et al. The protective effect of Baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis. BMCComplement Altern Med. 2014 Jan 13;14:19.2. Ju XN, et al. Baicalin protects against thrombin induced cell injury in SH-SY5Y cells. Int J Clin Exp Pa