美國(guó)FDA細(xì)菌學(xué)分析手冊(cè)第八版沙門(mén)氏菌

1、矮BAM: Sa襖lmonell疤aNOTICE:八If you 跋are loo擺king fo笆r BAM C骯hapter 礙5: 拜Salmone挨lla辦 (Decem擺ber 200般7 Editi擺on) tha扳t is in跋corpora跋ted by 半referen耙ce in 2安1 CFR P礙arts 16昂 and 11稗8: HYPERLINK /Food/FoodSafety/Product-SpecificInformation/EggSafety/EggSafetyActionPlan/ucm170615.htm 疤Federal柏 Regist佰er

2、Fina白l Rule扳1扒 (July 邦9, 2009柏, 74 FR瓣 33030)把: Preve翱ntion o哀f Salmo骯nella E岸nteriti安dis in 癌Shell E俺ggs Dur鞍ing Pro藹duction背, Stora絆ge, and昂 Transp壩ortatio絆n, plea埃se use 氨thesev藹ersions扒 of the罷 BAM HYPERLINK /downloads/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/UCM

3、244774.pdf 叭Salmone愛(ài)lla翱 Chapte愛(ài)r案2班 (PDF, 班189 Kb)扳and HYPERLINK /downloads/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/UCM244777.pdf 敗Appendi叭x 1: Ra岸pid Met拜hods fo阿r Detec胺ting Fo敖odborne搬 Pathog拜ens埃3百 (PDF, 哀195 Kb)靶.稗The mos瓣t recen俺tEditi凹o(jì)n of B翱AM Chap壩ter 5:

4、吧Salmone辦lla拔 is ava巴ilable 啊below t皚his not背ice.辦Novembe爸r 2011 隘Version版Bacteri八ologica敖l Analy捌tical M爸anual壩Chapter礙 5靶Salmone絆lla阿Authors愛(ài): Walla吧ce H. A盎ndrews 氨and HYPERLINK mailto:Thomas.Hammack 霸Thomas 絆Hammack俺Revisio斑n Histo阿ry藹:頒Novembe辦r 2011 巴- Addit伴ion to 凹Section鞍 C: Pre皚paratio頒n o

5、f fo隘ods for斑 isolat把ion of 熬Salmone澳lla辦: Leafy唉 green 邦vegetab哎les and罷 herbs.礙 班Februar懊y 2011 襖Remo敗ved lin搬k to Ap氨pendix 隘1: Rapi板d Metho骯ds for 骯Detecti案ng Food靶borne P爸athogen芭s (now 擺archive吧d). 稗D(zhuǎn)ecembe皚r 2007 瓣 Mamey邦 pulp m埃ethod a擺dded, a巴nd Sect隘ion D r八evised.案 叭June 20胺06 Eg愛(ài)gs meth愛(ài)

6、od revi芭sed for按 shell 愛(ài)eggs an矮d liqui癌d whole班 eggs. 柏April 2愛(ài)003 F鞍rog leg叭s metho扳d, Lact拔ic case笆in, Ren敗net cas捌ein, So藹dium ca翱seinate熬 and Ra熬bbit ca挨rcass m襖ethods 罷revised芭, top e懊ars and絆 other 襖dog che鞍w toys 拔added. 霸Removed拜 sectio爸n A.25,澳 Mechan背ical sh白aker. 盎October盎 25, 20藹01 Ex八t

7、ension斑 of the芭 applic哀ability斑 of the叭 orange哀 juice 案method 癌in sect瓣ion C.1癌9 to ap拔ple jui霸ce and 佰apple c半ider. 版1999-DE捌C, 2000巴-MAR, a愛(ài)nd 2000耙-AUG Fi奧nal rev礙ision o敗n 2000-班NOV-14 挨(see th稗e Intro哀duction板 for a 藹summary佰 of cha澳nges).阿To obta挨in a co靶py of a版 prior 胺version佰 not cu案rrently

8、擺 posted敖, pleas板e(cuò) conta昂ct HYPERLINK mailto:Thomas.Hammack 霸Thomas 暗Hammack澳Chapter背 Conten絆ts HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Intro#Intro l Intro#Intro 敗Introdu愛(ài)ction扒 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriologic

9、alAnalyticalManualBAM/ucm070149.htm#Equip#Equip l Equip#Equip 板Equipme罷nt and 唉Materia拔ls奧 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Media#Media l Media#Media 半Media a隘nd Reag案ents笆 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriolog

10、icalAnalyticalManualBAM/ucm070149.htm#Prep#Prep l Prep#Prep 班Prepara版tion of笆 foods 拔for iso靶l(wèi)ation 安of 昂Salmone芭lla版 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Isol#Isol l Isol#Isol 翱Isolati澳on of 百Salmone邦lla捌 HYPERLINK /Food/ScienceResearch/

11、LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Id#Id l Id#Id 斑Identif斑ication啊 of 隘Salmone氨lla拔 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 絆Referen礙ces啊Introdu艾ction瓣Several拜 change背s are b俺eing in挨trod

12、uce敖d in th吧is edit癌ion of 凹BAM (8頒th版 Editio八n). The柏 first 扮change 澳involve啊s the e翱xpanded凹 use of百 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063568.htm 昂Rappapo凹rt-Vass扳iliadis矮 (RV) m敖edium版4板 for fo佰ods wit半h both 懊high an隘d low l瓣evels o八f compe暗t

13、itive 阿microfl班ora. In暗 the pr隘evious 柏edition般, RV me奧dium wa俺s recom巴mended 笆only fo啊r the a襖nalysis昂 of shr跋imp. Ba凹sed on 霸the com礙pletion扒 of AOA把C preco吧llabora隘tive ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 靶5, 6

14、佰) and c埃ollabor愛(ài)ative ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 半7, 8版) studi斑es, RV 敗medium 爸is now 版being r頒ecommen岸ded for板 the an案alysis 俺of high皚 microb愛(ài)ial and版 low mi絆crobial俺 load f吧oods. R半V mediu拔m repla礙ces

15、sel八enite c搬ystine 敗(SC) br八oth for白 the an癌alysis 唉of all 斑foods, 搬except 矮guar gu昂m. In a挨ddition敖, RV me凹dium re拔places 班l(xiāng)auryl 霸tryptos敗e broth吧 for us皚e with 愛(ài)dry act瓣ive yea艾st. HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063686.htm 凹Tetrath版ionate 盎

16、(TT)岸5疤 broth 傲continu昂es to b昂e used 伴as the 稗second 翱selecti吧ve enri吧chment 白broth. 澳However叭, TT br鞍oth is 邦to be i埃ncubate鞍d at 啊43C霸 for th藹e analy懊sis of 懊high mi懊crobial熬 load f疤oods an扳d at 板35C凹 for th疤e analy哎sis of 盎low mic澳robial 背load fo隘ods, in骯cluding挨 guar g搬um.罷The sec敗ond cha襖nge i

17、nv稗olves t拌he opti敖on of r熬efriger壩ating i扳ncubate熬d preen懊richmen盎ts and 般selecti哀ve enri罷chments擺 of low骯-moistu伴re food搬s for u愛(ài)p to 72骯 h. Wit叭h this 百option,伴 sample安 analys白es can 版be init埃iated a澳s late 百as Wedn靶esday o柏r Thurs百day wit案hout we白ekend w絆ork bei伴ng invo拔lved.稗The thi叭rd chan骯ge

18、invo半lves re拌ducing 癌the per藹iod of 皚incubat把ion of 叭the HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064484.htm 懊lysine 半iron ag胺ar (LIA叭)捌6疤 slants辦. In th襖e forme版r editi半on (BAM柏-7), HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnaly

19、ticalManualBAM/ucm063699.htm 百triple 柏sugar i哀ron aga澳r (TSI)板7芭 and LI隘A slant岸s were 皚incubat唉ed at 芭35C拌 for 24耙 2 h 哎and 48 澳 2 h, 氨respect叭ively. 版Unpubli傲shed da疤ta have百 demons胺trated 百that th扳e 48 h 澳reading愛(ài) of LIA癌 slants扳 is wit壩hout di昂agnosti耙c value唉. Of 19版3 LIA s安lants e扳xamined辦, al

20、l g吧ave def懊initive斑 result吧s withi罷n 24 搬2 h of 扮incubat安ion. No岸 signif翱icant c芭hanges 靶altered案 the fi鞍nal tes岸t resul阿t when 辦the sla扳nts wer瓣e incub疤ated an霸 additi靶onal 24扮 h. Thu罷s, both邦 the TS般I and L哀IA slan背ts are 案now inc佰ubated 胺for 24 皚 2 h.藹The fou藹rth cha疤nge inv稗olves t疤he proc皚edur

21、e f澳or surf哀ace dis盎infecti阿on of s敖hell eg白gs. In 艾the pre芭vious e扳dition 霸(BAM-7)按, egg s耙hells w拌ere sur耙face-di搬sinfect絆ed by s扒oaking 凹in 0.1%胺 mercur拔ic chlo隘ride so佰lution 邦for 1 h拔 follow阿ed by s暗oaking 安in 70% 笆ethanol霸 for 30翱 min. M傲ercuric拔 chlori吧de is c哀lassifi挨ed as a扒 hazard白o(hù)us was半

22、te, and邦 is exp板e(cuò)nsive 扳to disp鞍ose of 擺accordi般ng to E岸nvironm絆ental P癌rotecti半on Agen鞍cy guid稗elines.吧 In thi絆s editi敖on (BAM唉-8) egg隘 shells扒 are no巴w surfa埃ce-disi絆nfected盎 by soa疤king fo邦r at le霸ast 10 疤sec in 扒a 3:1 s笆olution靶 consis阿ting of巴 3 part阿s of 70佰% alcoh背ol (eth骯yl or i壩sopropy壩l) t

23、o 1敖 part o安f iodin疤e/potas靶sium io邦dide so靶l(wèi)ution.唉The fif拌th chan艾ge invo般lves th熬e sampl斑e prepa叭ration 阿of eggs哀. Egg c胺ontents拌 (yolk 按and alb背umen) a拌re thor八oughly 熬mixed b鞍efore a巴nalysis拜. After盎 mixing版 the eg愛(ài)g conte奧nts, 罷25 g擺 (ml) a澳re adde壩d to 22跋5 ml tr暗ypticas啊e (tryp背tic) so頒y bro

24、th柏 supple辦mented 般with fe百rrous s爸ulfate.捌A metho敗d for t擺he anal絆ysis of稗 guar g氨um has 癌been in吧cluded.笆 When g扮uar gum哀 is pre般enriche哀d at a 按1:9 sam瓣ple/bro柏th rati辦o, a hi芭ghly vi辦scous, 半nonpipe愛(ài)ttable 骯mixture瓣 result巴s. Addi稗tion of澳 the en爸zyme ce瓣llulase阿 to the傲 preenr靶ichment巴 medium擺,

25、 howev癌er, res扮ults in澳 a read扳ily pip斑ettable疤 mixtur爸e.般A metho皚d for o百range j藹uice (p佰asteuri扳zed and伴 unpast班eurized啊) has b挨een inc盎luded d礙ue to r岸ecent o扒range j壩uice-re奧lated o隘utbreak懊s.辦The dir俺ections爸 for pi邦cking c邦olonies般 from t佰he sele鞍ctive p佰lating 鞍agars h挨ave bee邦n made 佰more ex

26、敗plicit 柏to refl翱ect the岸 intent隘 of the敖 method背. In th背e absen佰ce of t邦ypical 鞍or susp翱ect col版onies o盎n the s敗electiv跋e plati伴ng agar半s, it i案s recom傲mended 扳that at安ypical 靶colonie矮s be pi白cked to扒 TSI an邦d LIA s稗lants. 柏This re邦commend岸ation i般s based埃 on the拌 fact t拜hat up 懊to 4% o芭f all 扳Salm

27、one八lla拜 cultur佰es isol般ated by隘 FDA an扳alysts 班from ce罷rtain f芭oods, e扳special吧ly seaf扮oods, d百u(mài)ring t捌he past版 severa阿l years壩 have b笆een aty矮pical.唉Finally拌, since爸 the pu般blicati頒on of B藹AM-7, a翱 6-way 矮compari版son was俺 conduc唉ted of 百the rel百ative e藹ffectiv百eness o芭f the t疤hree se藹lective按 plat

28、in矮g agars半 recomm霸ended i捌n the B擺AM ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063348.htm 般bismuth氨 sulfit扮e伴8扒, HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064342.htm 昂Hektoen瓣 enteri疤c爸9俺, and HYPERLINK /F

29、ood/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062986.htm 辦xylose 扒lysine 叭desoxyc拌holate 襖agars昂10百) and t昂hree re阿lativel吧y new a敖gars (E靶F-18, x扮ylose l罷ysine T背ergitol稗 4, and佰 Rambac笆h agars傲). Our 扮results扒 (9) in阿dicated襖 no adv愛(ài)antage 佰in repl暗acing a懊ny of t皚he

30、BAM-芭recomme拌nded ag敗ars wit奧h one o骯r more 把of the 稗newer a柏gars. T按hus, th案e combi壩nation 霸of sele埃ctive p奧lating 爸agars r板e(cuò)commen拌ded in 跋BAM-7 r芭emains 昂unchang啊ed.奧Equipme跋nt and 頒Materia安ls暗 唉Blender拔 and st翱erile b敖lender 挨jars (耙see敗 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacterio

31、logicalAnalyticalManualBAM/ucm063335.htm 白Chapter稗 1唉11案) 壩Sterile叭, 巴16 oz挨 (500 m班l(xiāng)) wide敗-mouth,笆 screw-鞍cap jar懊s, ster辦ile 500埃 ml Erl盎enmeyer壩 flasks礙, steri矮le 250 笆ml beak襖ers, st拌erile g爸lass or跋 paper 巴funnels哎 of app背ropriat凹e size,稗 and, o八ptional凹ly, con懊tainers白 of app扒ropriat辦e capac吧

32、ity to 般accommo稗date co挨mposite凹d sampl胺es 邦Sterile拌, bent 敖glass o暗r plast佰ic spre俺ader ro矮ds 爸Balance罷, with 柏weights拜; 捌2000 g拔 capaci敗ty, sen盎sitivit啊y of 八0.1 g半 伴Balance叭, with 哎weights啊; 扒120 g叭 capaci矮ty, sen版sitivit氨y of 5 拜mg 叭Incubat稗or, 35 擺 擺2 C靶 皚Refrige氨rated i礙ncubato耙r or la埃borator

33、霸y refri半gerator敖, 4 懊2C奧 百Water b頒ath, 49鞍 白1C盎 暗Water b暗ath, ci擺rculati背ng, the拜rmostat胺ically-班control熬led, 43艾 挨0.2C敗 傲Water b挨ath, ci礙rculati暗ng, the疤rmostat岸ically-搬control凹led,42 哎 傲0.2C骯 稗Sterile鞍 spoons搬 or oth班er appr礙opriate挨 instru罷ments f哎or tran矮sferrin壩g food 阿samples拜 耙Sterile懊 cultu

34、r霸e dishe氨s, 15 x邦 敖100 mm跋, glass疤 or pla愛(ài)stic 凹Sterile爸 pipets柏, 1 ml,瓣 with 0矮.01 ml 唉graduat胺ions; 5板 and 10皚 ml, wi氨th 0.1 百ml grad襖uations爸 稗Inocula靶ting ne叭edle an挨d inocu拔lating 伴loop (a俺bout 斑3 mm扮 id or 耙10 藹5l澳), nich芭rome, p拔latinum辦-iridiu皚m, chro俺mel wir罷e, or s擺terile 藹plastic岸 疤Steri

35、le邦 test o骯r cultu耙re tube芭s, 16 x胺 搬150 mm癌 and 20叭 x 版150 mm罷; serol皚ogical 拜tubes, 斑10 x 辦75 mm瓣 or 13 伴x 耙100 mm皚 半Test or安 cultur哎e tube 扒racks 跋Vortex 靶mixer 唉Sterile鞍 shears版, large巴 scisso礙rs, sca挨lpel, a唉nd forc氨eps 骯Lamp (f胺or obse佰rving s搬erologi叭cal rea隘ctions)巴 巴Fisher 班or Buns捌en burn鞍

36、er 爸pH test板 paper 擺(pH ran癌ge 6-8)半 with m唉aximum 岸graduat胺ions of稗 0.4 pH吧 units 唉per col笆or chan耙ge 盎pH mete骯r 哎邦Plastic翱 bags, 奧28 x 拔37 cm案, steri按le, wit白h resea巴lable t搬ape. (I辦tems 23皚-24 are暗 needed埃 in the案 analys背is of f癌rog leg半s and r百abbit c擺arcasse佰s.) 八Plastic傲 beaker叭s, 4 li扳ter, au

37、骯toclava般ble, fo絆r holdi暗ng plas搬t(yī)ic bag胺 during板 shakin岸g and i疤ncubati艾on. 搬Sponges奧, non-b班acteric扒idal (N昂asco ca拔t # B01白299WA),笆 or equ捌ivalent拔. 艾Swabs, 搬non-bac白tericid熬al, cot百ton-tip芭ped.澳 HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm055778.htm 隘M

38、edia靶12岸 and HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm055791.htm 昂Reagent胺s澳13盎For pre拔paratio霸n of me搬dia and擺 reagen哎ts, ref搬er to M爸ethods 礙967.25礙-967.28昂 in藹 稗Officia奧l Metho唉ds of A埃nalysis隘 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bact

39、eriologicalAnalyticalManualBAM/ucm070149.htm#Refs#Refs l Refs#Refs 搬1唉).巴Lactose懊 broth 拔( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064414.htm 傲M74澳14藹) 襖Nonfat 瓣dry mil矮k (reco愛(ài)nstitut八ed) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriolog

40、icalAnalyticalManualBAM/ucm064144.htm 頒M111白15艾) 壩Selenit翱e cysti奧ne (SC)案 broth 翱( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063597.htm 版M134芭16埃) 皚Tetrath背ionate 阿(TT) br安oth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical

41、ManualBAM/ucm063686.htm 疤M145矮17柏) 奧Rappapo頒rt-Vass跋iliadis斑 (RV) m胺edium ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063568.htm 安M132埃18礙). NOTE跋: RV me瓣dium mu擺st be m伴ade fro捌m its i敗ndividu按al ingr罷edients背. Comme哎rcial f岸ormulat柏ions ar罷e not a捌ccep

42、tab擺le. 疤Xylose 氨lysine 阿desoxyc岸holate 敗(XLD) a扒gar ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062986.htm 半M179艾19敖) 罷Hektoen奧 enteri叭c (HE) 哎agar ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064342.htm 矮M61

43、靶20礙) 叭Bismuth搬 sulfit壩e (BS) 耙agar ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063348.htm 斑M(jìn)19埃21盎) 氨Triple 佰sugar i啊ron aga笆r (TSI)邦 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063699.htm 癌M149百22罷) 按Trypto

44、n巴e (tryp盎tophane巴) broth案 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063867.htm 哎M164版23叭) 笆Tryptic斑ase (tr藹yptic) 拜soy bro瓣th ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063805.htm 八M154安24翱) 霸Tryptic疤ase

45、soy稗 broth 安with fe班rrous s敗ulfate 半( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063816.htm 巴M186靶25霸) 半Tryptic班ase soy氨-trypto扒se brot案h ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063862.htm 壩M160稗26岸) 板MR-V

46、P b鞍roth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064068.htm 背M104暗27癌) 扮Simmons癌 citrat傲e agar 傲( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063639.htm 稗M138拜28盎) 盎Urea br跋oth ( HYPERLINK /Food/ScienceRese

47、arch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062935.htm 伴M171疤29擺) 霸Urea br柏oth (ra澳pid) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062967.htm 班M172澳30瓣) 把Malonat斑e broth哎 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriologic

48、alAnalyticalManualBAM/ucm064500.htm 拌M92敗31胺) 俺Lysine 佰iron ag澳ar (LIA笆) (Edwa八rds and阿 搬Fife藹) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064484.htm 盎M89擺32皚) 伴L(zhǎng)ysine 霸decarbo爸xylase 班broth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriolo

49、gicalAnalyticalManualBAM/ucm064474.htm 翱M87)絆33愛(ài) 扒Motilit啊y test 拜medium 巴(semiso辦lid) ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064059.htm 挨M103頒34擺) 愛(ài)Potassi邦um cyan岸ide (KC辦N) brot懊h ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/Bacteriologica

50、lAnalyticalManualBAM/ucm063514.htm 百M(fèi)126靶35傲) 哎Phenol 熬red car笆bohydra伴te brot稗h ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063494.htm 拜M121爸36昂) 頒Purple 骯carbohy扒drate b耙roth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical

51、ManualBAM/ucm063529.htm 安M130隘37版) 巴MacConk半ey agar昂 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064496.htm 背M91癌38靶) 靶Nutrien盎t broth癌 ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064169.htm 巴M114愛(ài)39矮) 唉Brain

52、 h斑eart in鞍fusion 辦(BHI) b斑roth ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063362.htm 阿M24班40半) 扒Papain 鞍solutio啊n, 5% ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm063490.htm 板M56a拌41矮) 皚Cellula啊se solu翱tion,

53、 1疤% ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm064568.htm 翱M187哀42背) 把Tryptos挨e blood半 agar b辦ase ( HYPERLINK /Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm062871.htm 盎M166癌43佰) 按Univers奧al pree伴nrichme拔nt brot爸h ( HYPER

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