Stattic - STAT 抑制劑 - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEStatticCat. No.: HY-13818CAS No.: 19983-44-9分式: CHNOS分量: 211.19作靶點(diǎn): STAT作通路: JAK/STAT Signaling; Stem Cell/Wnt儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 50 mg/mL (236.75 mM; Need ultra

2、sonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 4.7351 mL 23.6754 mL 47.3507 mL5 mM 0.9470 mL 4.7351 mL 9.4701 mL10 mM 0.4735 mL 2.3675 mL 4.7351 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn) 請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍福渲魄罢?qǐng)先配制澄清的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保

3、存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (11.84 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (11.84 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemE物活性 Stattic有效的 S

4、TAT3 活化和聚化的抑制劑。IC50 & Target STAT3體外研究 Stattic inhibits STAT3 phosphorylation (Y705) in ALDH+ and D44+/CD24+ subpopulations of Panc-1 andHPAC pancreatic cancer cell lines at a higher concenration (20 M). Stattic selectively inhibits P-STAT3 asdemonstrated by the lack of inhibition of P-ERK1/2 in bot

5、h ALDH+ and CD44+/CD24+ subpopulations ofPanc-1 and HPAC. Stattic inhibita STAT3 downstream targets in ALDH+ and CD44+/CD24+ subpopulationsof pancreatic cancer cells 1. Stattic reduces the LPS-induced expression of ICAM-1 and VCAM-1, andSTAT3 phosphorylation 2.PROTOCOLCell Assay 1 Pancreatic cancer

6、stem-like cells (3,000/well in 96-well plates) are incubated with desired concentrations ofcompounds in triplicate at 37C for 72 h. MTT viability assays are done and the absorbance is read at 595nm.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶(hù)使本產(chǎn)品發(fā)表

7、的科研獻(xiàn) Cell Metab. 2019 Jan 8;29(1):141-155.e9. Gut. 2019 May 10. pii: gutjnl-2018-317424. Mol Cancer. 2019 Mar 30;18(1):64. J Immunother Cancer. 2019 Jul 12;7(1):178. J Immunother Cancer. 2018 Dec 18;6(1):151. 2018See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Lin L, et al. STA

8、T3 as a potential therapeutic target in ALDH+ and CD44+/CD24+ stem cell-like pancreatic cancer cells. Int J Oncol.2016 Oct 12.2. Cho YS, et al. Inhibition of STAT3 phosphorylation by sulforaphane reduces adhesion molecule expression in vascular endothelial cell.Can J Physiol Pharmacol. 2015 Nov 18:1-7.McePdfHeightCaution: Product has not been fully validated for medical applications