Maslinic-acid-Crategolic-acid-DataSheet-生命科學試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEMaslinic acidCat. No.: HY-N0629CAS No.: 4373-41-5Synonyms: Crategolic acid; 2-Hydroxyoleanolic acid分式: CHO分量: 472.7作靶點: NF-B作通路: NF-B儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 28 mg/mL

2、 (59.23 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.1155 mL 10.5775 mL 21.1551 mL5 mM 0.4231 mL 2.1155 mL 4.2310 mL10 mM 0.2116 mL 1.0578 mL 2.1155 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Maslinic acid 可抑制 NF-B p65 的 DN

3、A 結(jié)合活性, 并消除 IB- 磷酸化。IC50 & Target p65體外研究Maslinic acid (MA) inhibits LPS-induced NF-B translocation to nucleus and phosphorylation of IB-.1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEMaslinic acid has also been reported to suppress NF-B regulated osteoclastogenesis in bone marrowmonocytes and in

4、hibit TNF-induced NF-B activity and its downstream genes expression in pancreaticcancer cells. To confirm if the anti-inflammatory effects of olive pomace extracts (OPEs) inRAW264.7 cellscan be attributed to Maslinic acid, dose-dependence experiments determined the effective concentration ofMaslinic

5、 acid to be 10-20 M. 20 M Maslinic acid significantly suppresses TNF- production and inhibits IL-1, IL-6, and COX-2 mRNA expression in RAW 264.7 cell. Maslinic acid (at 10 and 20 M) significantlysuppresses the DNA-binding activity of NF-B p65 in LPS-induced RAW 264.7 cells. Pretreatment withMaslinic

6、 acid significantly reduces the LPS-induced phosphorylation of IB- 1.體內(nèi)研究 Paw swelling is alleviated when mice are administered with 200 mg/kg Maslinic acid (MA), significantlysuppressing inflammation, compared to the carrageenan induced control group, 4 h after -carrageenaninjection (0.910.51 mm an

7、d 1.790.4 mm, respectively) 1.PROTOCOLCell Assay 1 RAW 264.7 cells are seeded in 96-well culture plates at a density of 1105 cells/mL and after incubation for24 h, are treated with OPE1 (300 g/mL or 400 g/mL), OPE2 (20 g/mL or 40 g/mL), or Maslinic acid (10 M or 20 M) , as well as with/without LPS a

8、t the same time. Cell viability i determined using the WST-1reagent. Briefly, WST-1 reagent (10 L) is added to each well and incubated for 1h in a humidified incubator.The absorbance of the samples measured at 450 nm (reference wavelength is 750 nm). Viability isexpressed as a percentage of the abso

9、rbance measured in LPS-treated cells 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice 1Administration 1 Five-week-old male Balb/c mice (19-21 g) are housed in a conventional condition and provided with the freeaccess to standard rodent chow

10、 and water. Edema is induced by intraplantar injection of 100 L 1%carrageenan into the hind left paw. Maslinic acid is tested initially at a dose of 200 mg/kg, orally administered60 min before and after carrageenan injection. Paw thickness is measured using electronic digital calipers, 2,3, and 4 h

11、following carrageenan treatment. Mice are sacrificed by carbon dioxide inhalation 4 h aftercarrageenan injection 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Fukumitsu S, et al. Anti-inflammatory and anti-arthritic effects of pentacyclic triterpenoids maslinic acid through NF-B inactivation. MolNutr Food Res. 2016 Feb;60(2):399-409.McePdfHeightCaution: Product has