索取part與提交part流程

1、art與提交part流程幫助文檔http:/Help:Contents11 Distribution KitsThe 2012 Distribution contains over 1000 part samples as dried (miniprepped) DNA, with each sample QC tested through sequencing, AB test plates, and restriction digests.http:/Help:Distribution_Kits2 1.1 2012年包含(5) 384w DNA Distribution Kit Plate

2、s Linearized Plasmid Backbones: pSB1A3, pSB1C3, pSB1K3.m1, pSB1T3 iGEM Stickers and Pins! (iGEM teams only) 31.2 Protocols（未看）3A Assembly KitCompetent CellsTransformationMiniprepRestriction DigestLigationLinearized Plasmid Backbones42 搜尋part搜尋basic part搜尋composite part搜尋部分part52.1 搜尋basic partpart n

3、umbername of the partbrowse for parts by typebrowse parts by function幫助文檔6part number789name of the part101112browse for parts by type13browse parts by function142.2 搜尋composite part 與搜尋basic part方法一樣151.3 How to find previously assembled parts that will aid in constructing a composite part 163 索取pa

4、rt幫助文檔17 3.1 搜尋18Available代表可以索取19不可索取20213.2 Quality Control質(zhì)量控制幫助文檔：22The Get This Part page is extremely useful when requesting a part sample. It will tell you: if your part sample is already included in your Distribution Kit if there are multiple samples for the part in the Registry any QC infor

5、mation tied to the part sample 23You can use the following information to email a request to hq (at) igem . org. The subject should be along the lines of Part Request: Your Teams Name. Be sure to include the following: iGEM team or lab name part name (BBa.) plasmid backbone and resistance sample loc

6、ation: source plate and well quality control information (if applicable): Sequence and Gel Result 24Things to keep in mindMany of the part samples available through the Registry have undergone quality control testing. Prior to requesting a part sample, take a look at this information, as it should a

7、ffect your decision on using it. See the quality control help page if you need help interpreting QC results on the “Get This Part” page. 在申請之前看清信息Prior to making a request it is helpful to consider all of the parts that the team needs for your project. We prefer bundling part requests together rathe

8、r than receiving several small requests, as this cuts down shipping costs and improves efficiency. 所有元件盡量一起索取Once a request is made you should be contacted by iGEM HQ within two business days. IGEM總部在兩天內(nèi)會聯(lián)系你253.3 Using agar stabs（瓊脂穿刺）We send out our part requests as agar stabsThe agar should alread

9、y have a hole from when it was stabbed. With an inoculating loop dipped into the stab, you can plate directly onto a petri dish with the appropriate antibiotic.Incubate the dish overnight at 37C (14-16hr)Pick a single colony to start up a cultureMiniprep to extract plasmid DNA 26實(shí)驗(yàn)組實(shí)現(xiàn)了解與準(zhǔn)備好轉(zhuǎn)移與保留培養(yǎng)流程

10、274 注冊新part幫助文檔http:/Help:Adding_Parts28在做出part前先在Registry注冊http:/Add_a_Part_to_the_Registry 添加文件29Add a Basic Part305 構(gòu)建part幫助文檔：http:/partsr/Help:Constructing_Parts/wiki/The_BioBricks_Foundation:Standards/Technical/Formats31 5.1 BioBrick所謂生物模塊（ BioBrick）是指經(jīng)標(biāo)準(zhǔn)化加工，具有標(biāo)準(zhǔn)的限制酶切割位點(diǎn)的元件、裝置和系統(tǒng)等。它也可以形象地稱為“生物

11、磚” 合成生物學(xué)與合成酶學(xué).張金等.科學(xué)出版社32標(biāo)準(zhǔn)化加工所謂標(biāo)準(zhǔn)化加工，是指所設(shè)計和構(gòu)建的元件、裝置和系統(tǒng)能夠協(xié)調(diào)匹配，規(guī)范連接，實(shí)現(xiàn)即插即用。元件需要用應(yīng)用前綴和后綴序列，其含有限制酶Ecor 、Xba 、Spe 和Not 切割位點(diǎn)。元件的核苷酸序列中不應(yīng)含上述限制酶的切割位點(diǎn)。 合成生物學(xué)與合成酶學(xué).張金等.科學(xué)出版社335.2 BioBrick RFC10標(biāo)準(zhǔn)化標(biāo)準(zhǔn)之一： BioBrick RFC 10（比賽規(guī)定）幫助文檔： http:/Help:Standards/Assembly/RFC10其它標(biāo)準(zhǔn)化過程：http:/wiki/The_BioBricks_Foundation:R

12、FC345.3 Basic Part 標(biāo)準(zhǔn)化前綴序列(有酶切位點(diǎn)）+part（需要標(biāo)準(zhǔn)化的序列）+后綴序列（有酶切位點(diǎn)）355.3.1 編碼蛋白質(zhì)區(qū)域的標(biāo)準(zhǔn)化365.3.2 其他序列的標(biāo)準(zhǔn)化375.4 Basic Part 標(biāo)準(zhǔn)化方法PCR標(biāo)準(zhǔn)化http:/Help:Protocol/PCR_StandardizationDNA合成http:/Help:Protocol/DNA_Synthesis定點(diǎn)誘變：改變序列中的illegal restriction sites385.5 Composite Parts標(biāo)準(zhǔn)化3A AssemblyScarless AssemblyGibson AssemblyStandard Assembly39提交part(郵寄）The Registry only accepts part samples in pSB1C3, the Registrys plasmid backbone shipping standard幫助文檔：4041424344454647*Important*: When shipping your samples, make sure to include a detailed desc