流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件

基于流式綱胞術(shù)的單細(xì)胞分析在臨床及科研中的應(yīng)用臺(tái)州市中心醫(yī)院醫(yī)學(xué)檢驗(yàn)科陳琪基于流式綱胞術(shù)的單細(xì)胞分析1流式細(xì)胞術(shù)的基本原理(一)流式細(xì)胞術(shù)的概念流式細(xì)胞術(shù)(f1owcytometry,FCM是利用流式細(xì)胞儀對(duì)生物顆粒包括細(xì)胞(紅細(xì)胞、白細(xì)胞、各類(lèi)組織細(xì)胞、血小板等)、微生物以及人工合成微球的多種物理和生物學(xué)特性進(jìn)行定量分析,并能對(duì)特定細(xì)胞群體加以分選的細(xì)胞參量分析技術(shù)。流式細(xì)胞術(shù)的基本原理2(二)FcM的工作原理1熒光染色(1)用熒光染料直接染色,如:Pl(2)用熒光抗體間接染色,FITC,PE2液流技術(shù):細(xì)胞懸液被吸入檢測(cè)室后,在鞘液的約束下,通過(guò)噴嘴,使其形成單細(xì)胞懸液。3激光技術(shù):散射光信號(hào)(FS、SS)熒光信號(hào)(FL1、FL2、FL3、FL4)4數(shù)據(jù)處理(二)FcM的工作原理3FlowCellInjectorFluorescenceFocusedlaserbeamFlowCell4FluorescenceDetectorsFluorescencedetector(PMT3,PMT4etc.)FluorescenceDetectors5流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件6流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件7流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件8流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件9流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件10流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件11流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件12流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件13流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件14流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件15流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件16流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件17流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件18流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件19流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件20流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件21流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件22流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件23流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件24流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件25流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件26流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件27流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件28流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件29流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件30流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件31流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件32流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件33流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件34流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件35流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件36流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件37流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件38流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件39流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件40流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件41流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件42流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件43流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件44流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件45流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件46流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件47基于流式綱胞術(shù)的單細(xì)胞分析在臨床及科研中的應(yīng)用臺(tái)州市中心醫(yī)院醫(yī)學(xué)檢驗(yàn)科陳琪基于流式綱胞術(shù)的單細(xì)胞分析48流式細(xì)胞術(shù)的基本原理(一)流式細(xì)胞術(shù)的概念流式細(xì)胞術(shù)(f1owcytometry,FCM是利用流式細(xì)胞儀對(duì)生物顆粒包括細(xì)胞(紅細(xì)胞、白細(xì)胞、各類(lèi)組織細(xì)胞、血小板等)、微生物以及人工合成微球的多種物理和生物學(xué)特性進(jìn)行定量分析,并能對(duì)特定細(xì)胞群體加以分選的細(xì)胞參量分析技術(shù)。流式細(xì)胞術(shù)的基本原理49(二)FcM的工作原理1熒光染色(1)用熒光染料直接染色,如:Pl(2)用熒光抗體間接染色,FITC,PE2液流技術(shù):細(xì)胞懸液被吸入檢測(cè)室后,在鞘液的約束下,通過(guò)噴嘴,使其形成單細(xì)胞懸液。3激光技術(shù):散射光信號(hào)(FS、SS)熒光信號(hào)(FL1、FL2、FL3、FL4)4數(shù)據(jù)處理(二)FcM的工作原理50FlowCellInjectorFluorescenceFocusedlaserbeamFlowCell51FluorescenceDetectorsFluorescencedetector(PMT3,PMT4etc.)FluorescenceDetectors52流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件53流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件54流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件55流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件56流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件57流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件58流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件59流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件60流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件61流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件62流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件63流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件64流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件65流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件66流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件67流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件68流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件69流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件70流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件71流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件72流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件73流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件74流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件75流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件76流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件77流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件78流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件79流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件80流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件81流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件82流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件83流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件84流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件85流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件86流式細(xì)胞術(shù)在臨床科研中應(yīng)用課件87流