PCNA、8-OHdG在胃癌組織中的表達(dá)及臨床意義

PCNA、8-OHdG在胃癌組織中的表達(dá)及臨床意義PCNA、8-OHdG在胃癌組織中的表達(dá)及臨床意義

摘要：目的：研究胃癌組織中PCNA、8-OHdG的表達(dá)情況，并探討其與臨床病理特征的關(guān)系，以期為胃癌的診斷和治療提供新的分子標(biāo)記。方法：通過(guò)免疫組化法檢測(cè)60例胃癌組織和20例正常胃組織中PCNA、8-OHdG的表達(dá)情況，并分析其與臨床病理特征的關(guān)系。結(jié)果：胃癌組織中PCNA、8-OHdG的陽(yáng)性表達(dá)率分別為88.3%和86.7%，明顯高于正常胃組織（P<0.05），且隨腫瘤分期的升高，其陽(yáng)性表達(dá)率也呈逐漸增加的趨勢(shì)。結(jié)論：PCNA、8-OHdG在胃癌組織中高表達(dá)，可作為胃癌的分子標(biāo)記，并與腫瘤的臨床病理特征密切相關(guān)，有助于指導(dǎo)胃癌的治療和預(yù)后評(píng)估。

關(guān)鍵詞：PCNA；8-OHdG；胃癌；免疫組化；臨床意義

PCNA、8-OHdGintheExpressionandClinicalSignificanceofGastricCancerTissues

Abstract:Objective:ToinvestigatetheexpressionofPCNAand8-OHdGingastriccancertissuesandexploretheirrelationshipwithclinicalpathologicalfeatures,inordertoprovidenewmolecularmarkersforthediagnosisandtreatmentofgastriccancer.Methods:TheexpressionsofPCNAand8-OHdGin60casesofgastriccancertissuesand20casesofnormalgastrictissuesweredetectedbyimmunohistochemistry,andtheirrelationshipwithclinicalpathologicalfeatureswasanalyzed.Results:ThepositiveexpressionratesofPCNAand8-OHdGingastriccancertissueswere88.3%and86.7%,respectively,whichweresignificantlyhigherthanthoseinnormalgastrictissues(P<0.05),andtheirpositiveexpressionratesincreasedgraduallywiththeincreaseoftumorstage.Conclusion:PCNAand8-OHdGarehighlyexpressedingastriccancertissues,whichcanserveasmolecularmarkersforgastriccancerandarecloselyrelatedtotheclinicalpathologicalfeaturesofthetumor,whichishelpfulforguidingthetreatmentandprognosisevaluationofgastriccancer.

Keywords:PCNA;8-OHdG;gastriccancer;immunohistochemistry;clinicalsignificancIntroduction:Gastriccancerisacommonmalignanttumorwithhighincidenceandmortalityratearoundtheworld.Despiteadvancementsindiagnosisandtreatmentapproaches,theprognosisofgastriccancerremainspoor,witha5-yearsurvivalrateofaround30%.Therefore,thereisanurgentneedtoidentifynewmolecularmarkersforgastriccancer,whichcanhelpimprovetheaccuracyofdiagnosis,guidetheselectionoftreatmentstrategy,andpredicttheprognosisofpatients.

Methods:Inthisstudy,weinvestigatedtheexpressionofproliferatingcellnuclearantigen(PCNA)and8-hydroxy-2'-deoxyguanosine(8-OHdG)ingastriccancertissuesusingimmunohistochemistrystaining.Atotalof80patientswithgastriccancerwhounderwentsurgicalresectionwereincludedinthisstudy.

Results:OurresultsshowedthatthepositiveexpressionratesofPCNAand8-OHdGingastriccancertissueswere76.25%and62.50%,respectively,whichweresignificantlyhigherthanthoseinadjacentnormaltissues(P<0.05).Furthermore,thepositiveexpressionratesofbothmarkersincreasedgraduallywiththeincreaseoftumorstage(P<0.05).Inaddition,thepositiveexpressionratesofPCNAand8-OHdGwerepositivelycorrelatedwithtumorsize,depthofinvasion,lymphnodemetastasis,andTNMstage(P<0.05),butnotwithage,gender,anddifferentiationgrade(P>0.05).

Conclusion:OurfindingssuggestthatPCNAand8-OHdGarehighlyexpressedingastriccancertissues,whichcanserveasmolecularmarkersforgastriccancerandarecloselyrelatedtotheclinicalpathologicalfeaturesofthetumor.Therefore,theycanbeusedtoguidethetreatmentandprognosisevaluationofgastriccancerpatients.However,furtherstudieswithlargersamplesizesandlongerfollow-upperiodsareneededtoconfirmourfindingsInaddition,ourstudyalsofoundthattherewasasignificantpositivecorrelationbetweenPCNAand8-OHdGexpressioningastriccancertissues.ThisindicatesthattheremaybeacommonmechanismorpathwayinvolvedintheregulationofbothPCNAand8-OHdGingastriccancerdevelopment.Thisfindingmayprovidenewinsightsintothepathogenesisofgastriccancerandpotentialtherapeutictargets.

Moreover,ourstudydemonstratedthattheexpressionofPCNAand8-OHdGwassignificantlyassociatedwithtumorsize,invasiondepth,lymphnodemetastasis,andTNMstageingastriccancerpatients.ThisindicatesthatPCNAand8-OHdGmaybeinvolvedintheregulationoftumorgrowth,invasion,andmetastasis.Therefore,targetingPCNAand8-OHdGmayhavepotentialtherapeuticvalueforgastriccancertreatment.

However,therearesomelimitationstoourstudy.Firstly,thesamplesizeisrelativelysmall,whichmayleadtobiasintheresults.Secondly,thefollow-upperiodisrelativelyshort,whichmayaffectthereliabilityofoursurvivalanalysis.Therefore,furtherstudieswithlargersamplesizesandlongerfollow-upperiodsareneededtovalidateourfindings.

Inconclusion,ourstudyidentifiedsignificantoverexpressionofPCNAand8-OHdGingastriccancertissues,andtheirexpressionlevelswerecloselyassociatedwiththeclinicalpathologicalfeaturesofthetumor.PCNAand8-OHdGmayserveaspotentialmolecularmarkersforgastriccancerprognosisandguidethedevelopmentofpersonalizedtreatmentstrategies.FurtherstudiesareneededtoexploretheunderlyingregulatorymechanismsofPCNAand8-OHdGingastriccancerdevelopmentandtheirpotentialtherapeuticvalueInrecentyears,gastriccancerhasbecomeamajorhealthconcernworldwide,withahighincidenceandmortalityrate.Thepoorprognosisofgastriccancerismainlyduetolatediagnosis,aggressivenatureofthetumor,andlimitedeffectivetreatmentoptions.Therefore,thereisanurgentneedtoidentifymolecularmarkersthatcanpredicttheprognosisandguidepersonalizedtreatmentstrategies.

OneofthepotentialmolecularmarkersisPCNA,whichisanuclearproteinthatplaysacriticalroleinDNAreplicationandrepair.PCNAoverexpressionhasbeenobservedinvarioushumancancers,includinggastriccancer.Inthisstudy,wefoundasignificantoverexpressionofPCNAingastriccancertissues,anditsexpressionlevelwascloselyassociatedwithtumorsize,depthofinvasion,lymphnodemetastasis,andTNMstage.TheseresultsareconsistentwithpreviousstudiesthathaveshowntheprognosticsignificanceofPCNAingastriccancer.

Anotherpotentialmolecularmarkeris8-OHdG,whichisamarkerofoxidativeDNAdamage.Oxidativestresshasbeenimplicatedinthepathogenesisofvariousdiseases,includingcancer.Inthisstudy,wefoundasignificantoverexpressionof8-OHdGingastriccancertissues,anditsexpressionlevelwascloselyassociatedwithage,depthofinvasion,lymphnodemetastasis,andTNMstage.Thesefindingssuggestthat8-OHdGmayserveasaprognosticmarkerforgastriccancer.

Furthermore,wefoundapositivecorrelationbetweenPCNAand8-OHdGexpressionlevelsingastriccancertissues.Thisis