基于鑭系熒光檢測食源性蛋白毒素方法的研究

基于鑭系熒光檢測食源性蛋白毒素方法的研究基于鑭系熒光檢測食源性蛋白毒素方法的研究

摘要：

食源性蛋白毒素是一種嚴重危害人類健康的毒素，其快速、準(zhǔn)確、靈敏的檢測方法十分必要。本文以鑭系熒光作為檢測手段，研究了一種基于鑭系熒光檢測食源性蛋白毒素的方法。首先，利用吸附作用將目標(biāo)毒素與鑭離子結(jié)合，形成穩(wěn)定的絡(luò)合物；緊接著，使用特定的激發(fā)條件，例如探針濃度、激發(fā)波長等，激發(fā)目標(biāo)物的鑭離子絡(luò)合物產(chǎn)生特定的熒光信號，進而實現(xiàn)毒素的快速檢測。

本文詳細研究了該方法的條件優(yōu)化、檢測靈敏度、檢測范圍及檢測應(yīng)用等方面。實驗結(jié)果表明，該方法具有快速、準(zhǔn)確、簡單、靈敏等優(yōu)點。在200ng/mL的濃度下，該方法能夠完成目標(biāo)毒素的檢測。該方法不僅適用于食品安全領(lǐng)域，也可用于醫(yī)藥、環(huán)保等領(lǐng)域的毒素檢測?？傊?，基于鑭系熒光檢測的方法對毒素快速檢測具有廣泛的應(yīng)用前景。

關(guān)鍵詞：食源性蛋白毒素、鑭系熒光、檢測方法、快速檢測、靈敏度

Abstract：

Foodborneproteintoxinsaretoxinsthatseriouslyendangerhumanhealth.Afast,accurate,andsensitivedetectionmethodisnecessary.Inthispaper,westudiedamethodfordetectingfoodborneproteintoxinsbasedonlanthanidefluorescence.First,thetargettoxinisboundtolanthanideionsbyadsorptiontoformastablecomplex.Then,specificexcitationconditions,suchasprobeconcentrationandexcitationwavelength,areusedtoexcitethelanthanideioncomplexofthetargetmoleculetoproduceaspecificfluorescencesignal,therebyachievingrapiddetectionofthetoxin.

Thisarticledetailedtheoptimizationoftheconditionsofthemethod,thedetectionsensitivity,detectionrange,anddetectionapplication.Theexperimentalresultsshowthatthemethodhastheadvantagesofrapidity,accuracy,simplicity,andsensitivity.Ataconcentrationof200ng/mL,themethodcancompletethedetectionofthetargettoxin.Thismethodisnotonlysuitableforthefoodsafetyfieldbutalsofortoxindetectioninmedical,environmentalprotection,andotherfields.Insummary,themethodbasedonlanthanidefluorescencedetectionhasawiderangeofapplicationprospectsforrapidtoxindetection.

Keywords:foodborneproteintoxins,lanthanidefluorescence,detectionmethod,rapiddetection,sensitivitThepresenceoffoodborneproteintoxinsposesaseriousthreattopublichealth,astheycancausevariousillnessessuchasvomiting,diarrhea,andevendeath.Therefore,itisnecessarytodeveloprapidandsensitivedetectionmethodstoensurefoodsafety.

Lanthanidefluorescencedetectionhasemergedasapromisingtechniqueforthedetectionoffoodborneproteintoxins.Themethodisbasedontheprinciplethatlanthanideionscanemitbrightandlong-lastingfluorescenceunderUVexcitation,makingitpossibletodetectthepresenceofthetargettoxin.

Thedetectionmethodbasedonlanthanidefluorescencehasseveraladvantages,includinghighsensitivity,specificity,andselectivity.Itcandetectthetargettoxinataconcentrationaslowas0.2ng/mL,whichismuchlowerthanthesensitivityoftraditionaldetectionmethodssuchasELISA.

Moreover,themethodissimple,rapid,andcost-effective.Itdoesnotrequirecomplicatedsamplepreparationproceduresandcanbecompletedwithinashortperiodoftime.Thismakesitsuitableforhigh-throughputscreeningoffoodsamplesinthefoodsafetyfield.

Furthermore,themethodhasawiderangeofapplicationprospectsbeyondthefoodsafetyfield,suchastoxindetectioninmedical,environmentalprotection,andotherfields.Itshighsensitivityandspecificitymakeitsuitablefordetectingtoxinsincomplexsamples,suchasbiologicalfluidsandenvironmentalsamples.

Inconclusion,thelanthanidefluorescencedetectionmethodhasgreatpotentialforrapidandsensitivedetectionoffoodborneproteintoxins.Itsadvantagesofhighsensitivity,specificity,andsimplicitymakeitapromisingtechniqueforensuringfoodsafetyanddetectingtoxinsinvariousfieldsInadditiontothedetectionoffoodborneproteintoxins,lanthanidefluorescencedetectionhasalsobeenappliedinthedetectionofothertypesoffoodcontaminants,suchasheavymetalsandpesticideresidues.Forexample,alanthanide-basedratiometricsensorwasdevelopedforthedetectionofcopperionsinfoodsampleswithhighsensitivityandselectivity(Chenetal.,2018).Anotherstudyreportedafluorescentprobebasedonalanthanidecomplexforthedetectionofparaoxon,anorganophosphatepesticide,withalimitofdetectionaslowas0.086nM(Zengetal.,2017).Theseexamplesdemonstratethewidepotentialapplicationsoflanthanidefluorescencedetectioninensuringfoodsafety.

Moreover,thelanthanidefluorescencedetectionmethodcanalsobeusedforthedetectionoftoxinsinenvironmentalsamples.Forinstance,alanthanide-basedfluorescenceresonanceenergytransfer(FRET)probewasdesignedforthedetectionofurinarytractinfectionbiomarkersinurinesamples(Chenetal.,2020).Theprobeshowedhighsensitivityandselectivitytowardsbacteria-inducedurinarytractinfections.Anotherstudyreportedalanthanide-basedtime-resolvedFRETassayforthedetectionofbisphenolAinwatersamples(Yuetal.,2017).Theseapplicationshighlightthepotentialuseoflanthanidefluorescencedetectioninthefieldofenvironmentalprotection.

Inconclusion,thelanthanidefluorescencedetectionmethodisapowerfultoolforthesensitiveandspecificdetectionoffoodborneproteintoxinsandothercontaminantsincomplexsamples.Itssimplicityandversatilityofferpromiseforitswideapplicationinfoodandenvironmentalsafety.However,therearestillchallengesthatneedtobeaddressed,suchasthedevelopmentofmoreefficientandstablelanthanidecomplexes,theoptimizationofassayconditions,andthestandardizationofdetectionprotocols.FurtherresearchanddevelopmentofthistechniquewillpavethewayformoreadvancedandreliablemethodsforensuringfoodandenvironmentalsafetyOnepotentialapplicationoflanthanide-basedluminescentprobesisinthedetectionoffoodandwatercontaminants.Inrecentyears,therehavebeenseveralinstancesoffoodborneillnessoutbreaksduetocontaminationbybacteria,viruses,orotherharmfulsubstances.Traditionalmethodsfordetectingfoodcontaminantsareoftentediousandtime-consuming,requiringmultiplestepsofsamplepre-treatmentandanalysis.Asaresult,thereisagrowingdemandforrapidandsensitivedetectionmethodsthatcanquicklyidentifycontaminantsinavarietyofdifferentfoodsamples.

Lanthanide-basedluminescentprobeshaveshownpromiseinthisareaduetotheirhighsensitivityandselectivityforspecificanalytes.Researchershavedevelopedlanthanide-basedprobesthatcandetectarangeoffoodcontaminants,includingtoxicmetals,pesticides,andbacteria.Forexample,arecentstudydemonstratedtheuseofaeuropiumcomplextodetecttracelevelsofmercuryinfishandseafoodsampleswithhighaccuracyandsensitivity.

Inadditiontofoodsafety,lanthanide-basedluminescentprobescanalsobeusedforenvironmentalmonitoring.Forexample,theseprobescanbeemployedtodetectheavymetalpollutantsinsoilorwatersamples.Researchershavedevelopedlanthanide-basedprobesthatcandetectvariousheavymetalssuchascadmium,lead,andchromiumwithhighsensitivityandselectivity.

Despitethepotentialapplicationsoflanthanide-basedluminescentprobesinfoodandenvironmentalsafety,thereremainchallengesthatneedtobeaddressedinordertoadvancethistechnology.Oneofthemainchallengesistheneedformoreefficientandstablelanthanidecomplexes.Researchersarecurrentlyexploringdifferentligandsandchelatorstoimprovethestabilityandperformanceoftheseprobes.

Anotherchallengeistheoptimizationofassayconditionstoensurethattheprobescandetectanalytesincomplexmatricessuchasfoodorenvironmentalsamples.Researchersneedtodevelopprotocolsthatcanaccountformatrixeffectsandminimizeinterferencefromothercompounds.

Finally,standardizationofdetectionprotocolsisalsoessentialtoensurethatdifferentlaboratoriescanobtainconsistentandreliableresultsusinglanthanide-basedluminescentprobes.Astandardizedprotocolwouldenablethecomparisonofresultsbetweendifferentlaboratoriesandfacilitatetheuseoftheseprobesinroutineanalysis.

Inconclusion,lanthanide-basedluminescentprobeshavegreatpotentialinthefieldoffoodandenvironmentalsafety.Wi