獸醫(yī)師貓傳染病專題中文7workshop diagnosis esavs katrin

OverviewofInfectiousDiseaseDiagnostics(Titers,PCR) KatrinProf.Dr.med.vet.,Dr.habil.,Dipl.ECVIM- Diagnostic viralDNA/RNAviralprotein

的cell-boundantigen細胞-indirectdetectionof cellular Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen

indirectpathogen間接檢病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen

indirectpathogen間接檢病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogenwholepathogenpartsofthe

indirectpathogen間接檢病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogenwholepathogenpartsofthe

indirectpathogen間接檢病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogenwholepathogenpartsofthe

indirectpathogen間接檢病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogenwholepathogenpartsofthe

indirectpathogen間接檢病immuneHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogenwholepathogenpartsofthe

indirectpathogen間接檢病immuneoftheHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogenwholepathogenpartsofthe

indirectpathogen間接檢病immuneoftheWhatistheproblemofindirectpathogenWhatistheproblemofindirectpathogenWhatistheproblemofindirectpathogenHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病

indirectpathogen間接檢測病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病

indirectpathogen間接檢測病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病

indirectpathogen間接檢測病detectionofthewholedetectionofdetectionof檢測病原的Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogendetectionthewholeHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogendetectionthewholeHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病1)detectionthewhole-Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病detectionthewhole-Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病detectionthewhole-electronHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病detectionthewhole-electronHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病detectionoftheproteins-Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病2detectionoftheproteins-Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病detectionoftheIFA免疫熒光抗體測Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病detectionof檢測病原的Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病directpathogen直接檢測病3)detectionof檢測病原的-Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病direct

indirect直接檢測病

間接檢測病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病direct

indirect直接檢測病

間接檢測病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病direct

indirect直接檢測病

間接檢測病detectionofdetectionofcellularimmunityHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病indirectpathogen間接檢測病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病indirectpathogen間接檢測病detectionofIFA免疫熒光抗體WesternHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病indirectpathogen間接檢測病detectionofIFA免疫熒光抗體WesternHowcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病indirectpathogen間接檢測病Howcananinfectiousdiseasebediagnosed?如何能診斷傳染性疾病indirectpathogen間接檢測病detectionofcellularimmunityintradermal建立診斷的可能bloodantigentests抗原測antibody抗體測Blood血涂片/細胞Antigen Antibody Inwhichinfectionsisanantibodytestuseful?在哪 時，抗體測試有用WhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited WhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatsWhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatsWhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatslife-longWhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatslife-longWhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatslife-longnoWhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatslife-longnoWhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatslife-longnoincatsolderthan6monthsmaternalWhyisanantibodytestusefultodiagnoseFIVinanadultcat(outsideoftheUnited infectedcatslife-longnoincatsolderthan6monthsmaternal大于6個月的貓沒有母源抗Whyisanantibodytestnotusefultodiagnosepanleukopenia?為什么抗體測試對診斷泛白細胞減少癥沒Whyisanantibodytestnotusefultodiagnosepanleukopenia?為什么抗體測試對診斷泛白細胞減少癥沒acuteWhyisanantibodytestnotusefultodiagnosepanleukopenia?為什么抗體測試對診斷泛白細胞減少癥沒acute Whyisanantibodytestnotusefultodiagnosepanleukopenia?為什么抗體測試對診斷泛白細胞減少癥沒acute self-limitingWhyisanantibodytestnotusefultodiagnosepanleukopenia?為什么抗體測試對診斷泛白細胞減少癥沒acute self-limiting自 Whyisanantibodytestnotusefultodiagnosepanleukopenia?為什么抗體測試對診斷泛白細胞減少癥沒acute self-limiting自 mostcatsarevaccinatedWhyisanantibodytestnotusefultodiagnosepanleukopenia?為什么抗體測試對診斷泛白細胞減少癥沒acute self-limiting自 mostcatsarevaccinatedorhavesurvived或者已經(jīng) 中幸存下Isthereanyuseindetectionantibodiesagainstpanleukopenia?檢測泛白細胞減少癥抗體有使用價值Isthereanyuseindetectionantibodiesagainstpanleukopenia?檢測泛白細胞減少癥抗體有使用價值Isthereanyuseindetectionantibodiesagainstpanleukopenia?檢測泛白細胞減少癥抗體有使用價值canpredictIsthereanyuseindetectionantibodiesagainstpanleukopenia?檢測泛白細胞減少癥抗體有使用價值canpredictCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值Canweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofpairedsamples–4-foldCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofpairedsamples–4-foldCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofpairedsamples–4-folddifferentiationofIgMandCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofpairedsamples–4-folddifferentiationofIgMandCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofpairedsamples–4-folddifferentiationofIgMandantibodytestsinotherbodyCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofpairedsamples–4-folddifferentiationofIgMandantibodytestsinotherbodyCanweincreasethevalueofantibodytests?我們能提高抗體測試的使用價值detectionofatiter–heightofpairedsamples–4-folddifferentiationofIgMandantibodytestsinotherbodyDeterminationofIgMandIgGDeterminationofIgMandIgGDeterminationofIgMandIgGDeterminationofIgMandIgGDeterminationofIgMandIgGDiagnosticOptions detection直 整 isolation電鏡 viralDNA/RNA檢 的 viralprotein檢 的蛋白antigenindirectdetectionofantibodiesELISA,IFA,WesternBlot蛋白質(zhì)cellularimmunity細胞免疫skintestingDiagnosticOptions detection直 整 isolation電鏡 viralDNA/RNA檢 的 viralprotein檢 的蛋白antigenindirectdetectionofantibodiesELISA,IFA,WesternBlot蛋白質(zhì)cellularimmunity細胞免疫skintesting Infectionsfeline infectionfeline infectionfelineinfectiousperitonitisfeline infectionfeline infectionfelinepanleucopenia Infectionsfeline infectionfeline infectionfelineinfectiousperitonitisfeline infectionfeline infectionfelinepanleucopeniaDiagnosticOptionsFIV viralDNA/RNAviralprotein

的原cell-bound indirectdetectionof cellularinvalidtestsdifficult敏感特異pos.predictive陽性預測值neg.FIVTestComparisono???invalidteststestsdifficulttointerpretsensitivityspecificitypos.predictivevalue(%)neg.predictivevalue(%)o?o???invalidteststestsdifficulttointerpretsensitivityspecificitypos.predictivevalue(%)neg.valueWhycananantibodytestbeusedtodiagnoseinfection?抗體測試為什么能用于診 Whycananantibodytestbeusedtodiagnoseinfection?抗體測試為什么能用于診 infectedcatsWhycananantibodytestbeusedtodiagnoseinfection?抗體測試為什么能用于診 infectedcatsWhycananantibodytestbeusedtodiagnoseinfection?抗體測試為什么能用于診 infectedcatslife-longnoWhycananantibodytestbeusedtodiagnoseinfection?抗體測試為什么能用于診 infectedcatslife-longno終 Whycananantibodytestbeusedtodiagnoseinfection?抗體測試為什么能用于診 infectedcatslife-longno終 noWhycananantibodytestbeusedtodiagnoseinfection?抗體測試為什么能用于診 infectedcatslife-longno終 noInwhichcatsdoesthepresenceofantibodiesNOTindicateinfection?在哪些貓存在抗體并不表示有？InwhichcatsdoesthepresenceofantibodiesNOTindicateinfection?在哪些貓存在抗體并不表示有？kittensupto6InwhichcatsdoesthepresenceofantibodiesNOTindicateinfection?在哪些貓存在抗體并不表示有？kittensupto6monthsInwhichcatsdoesthepresenceofantibodiesNOTindicateinfection?在哪些貓存在抗體并不表示有？kittensupto6monthsvaccinatedcatsInwhichcatsdoesthepresenceofantibodiesNOTindicateinfection?在哪些貓存在抗體并不表示有？kittensupto6monthsvaccinatedcats接種過的貓DiagnosticOptionsFIVdiagnostictestof選擇診斷測antibodies 問maternal ? 其他選DNAFel-O-VaxFIV?(Fortinactivated vaccine subtype

亞型APetaluma亞型BShizuoka82%effectiveagainstsuptypeA(exp.sofaronlyavailablein至今只 producedantibodiesnotdistinguishablefrominfection-inducedantibodies產(chǎn)生的抗體不能 PCRasPCRasUSALevyetal.,PCRasUSALevyetal.,2007testingof300catswithknownFIVstatusPCRasUSALevyetal.,2007testingof300catswithknownFIVstatusin5commerciallabsPCRasUSALevyetal.,2007testingof300catswithknownFIVstatusin5commerciallabs在5個商 對300只已知的FIV貓 PCRasUSALevyetal.,2007testingof300catswithknownFIVstatusin5commerciallabs在5個商 對300只已知的FIV貓 highnumberoffalse 較高的假陽性PCRasUSALevyetal.,2007testingof300catswithknownFIVstatusin5commerciallabs在5個商 對300只已知的FIV貓 =>highnumberoffalse=>highnumberoffalse

較高的 PCRasUSALevyetal.,2007testingof300catswithknownFIVstatusin5commerciallabs在5個商 對300只已知的FIV貓 highnumberoffalse 較高的假陽性=>highnumberoffalse 較高的 onlybetween50and80%oftheofinfectedcatsdiagnosedas PCRasGermanyHartmannetal2001testingof83FIV-infectedcatsbyrealtimePCR對83只 的貓作實時PCR測using3differentprimer用3種不同的引PCRasGermanyHartmannetal2001testingof83FIV-infectedcatsbyrealtimePCR對83只 的貓作實時PCR測using3differentprimer用3種不同的引only40of83PCR-positive!

Discriminant=>differentiateFIV-vaccinatedfromFIV-infected fyingantibodyreactivityto2對2種FIV抗原的抗體反應性進行定量測formalin-fixedsynthetictransmembrane(TM)all162uninfectedcatscorrectly162只 =>specificity100特異性99/102FIV-infectedcatscorrectly102 貓當中有99只被鑒定出 貓felineimmunodeficiency infectionFIV)貓feline infectionfelineinfectiousperitonitisfeline infectionfeline felinepanleucopeniaDiagnosticOptionsFeLV viral viral antigencell-bound indirectdetectionofAntibodiescellularimmunityFeLVTestComparisono???invalidtestsdifficultto敏感特異pos.predictiveneg.predictiveo??o???invalidtestsdifficult敏感特異pos.陽性預測值neg.?????invalidtestsdifficult敏感特異pos.predictiveneg.WhatdoestheFeLVtestantigentestdetectscoreprotein檢 蛋白WhyisantigendetectionpossibleincaseofFeLV?為什么抗原測試可以用在FeLV診斷DiagnosticOptionsFeLVdiagnostictestofantigen游離抗原Problemslatent潛在interpretationofdiscrepantresultsELISA/PCR結(jié)果 ，如何解釋?alternativesDNADiscrepantResultsstudyin 進行的研究Hoffmann-Lehmannetal.,2.4%ofcatsFeLVantigen-2.4%的貓FeLV抗原-陽10.0%additionally -positive(antigen--此外10.0% -陽性（抗原 interpretationfalse-negativeantigen 假 抗原結(jié)果latentlyinfected 潛 貓couldthesecatspotentiallyexcrete 可能這些貓有潛在排毒的可能 eofInfection p27antigen

(?regressorregressive好轉(zhuǎn)(?latent潛在的

transiently一過性的陽positive陽

免疫反strongstrongno多mann-Lehmannetal.,DiscrepantResultsDiscrepantResultsDiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 antibodiesantip45)抗體（抗DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 antibodiesantip45)抗體（抗0%ofantigen-positive, DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 antibodiesantip45)抗體（抗0%ofantigen-positive, DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 antibodiesantip45)抗體（抗0%ofantigen-positive, -17.4%ofantigen-negative, DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 antibodiesantip45)抗體（抗0%ofantigen-positive, -17.4%ofantigen-negative, 抗原 ，同時 DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 antibodiesantip45)抗體（抗0%ofantigen-positive, 17.4%ofantigen-negative, 抗原 ，同時 60.0%ofantigen-negative, DiscrepantResultsstudyin 1.8%ofcatsFeLVantigen-1.2%additionally -此外1.2% Shedding(RNAPCRinsaliva唾液RNAnoantigen-negativecatwas沒有抗 antibodiesantip45)抗體（抗0%ofantigen-positive, 17.4%ofantigen-negative, 抗原 ，同時 60.0%ofantigen-negative, eofInfection

p27antigen抗原

前

Abortive(?regressor

strong

transiently一過性的陽positive陽

陽性Hofmann-間陽性Hofmann-

strongno可

690etal., feline infectionfeline infectionfelineinfectiousperitonitisfeline infectionfeline infectionfelinepanleucopeniaDiagnosticOptionsFIP 直 檢 viralviral

的cell-bound indirectdetectionofcellular

FCoVAntigenin 抗incatswitheffusionimmunofluorescence-centrifugeaspirate(1500-spreadonaslide(thick-在玻片上擴散（厚層-fixateinacetoneat-20- 在-20°C固-stainwithanti-FITCconjugate用抗-FCoV抗體FITC-usefluorescence (450FCoVAntigenin巨噬細胞中的貓冠 抗diagnosticstudy(109catswithFIP,62catsnot診斷學研Hartmannetal.,sensitivity57敏感性specificity100特異性neg.predictivevalue57預測值pos.predictivevalue100陽性預測值FCoVAntigenin巨噬細胞中的貓冠 抗incatswithouttissuepos.predictivevalue100DiagnosticOptionsFIPdiagnostictestof選擇診斷測cell-boundantigenin 問macrophagesincatswithout?alternatives其PCRantibodies???FCoVPCRin血液的FCoV“reasonsfor 做PCR的“理由detectionofthemutatedbecause…突 的檢測，因為onlythe causes只有突 會引起detectionofthe inbloodbecause…血液中 測試，因為onlytheFIP- makes只有致 才會造 血LongTermPCRStudy(1247catswithconfirmedFIP

Gunn-Mooreetal.,69healthycatsofendemichouseholds80%viremic(PCRpositive)inboth independentofhealthviremiadoesnotpredictdevelopmentof PCRversusIFAWhyisthedetectionofintracellularantigen(IFA)inmacrophagesdiagnosticandthedetectionofviralRNAnot Antibody抗體測Antibody抗體測blood=>rule-outAntibody抗體測blood=>rule-out=>排除Antibody抗體測blood=>rule-out=>排除Antibody抗體測blood=>rule-out=>排除滲出Antibody抗體測blood=>rule-out=>排除滲出Antibody抗體測blood=>rule-out=>排除滲出腦脊Anti-FCoVAntibodiesindiagnosticvalueofantibodiesinFoleyetal.,catswithotherneurologicdiseasescanhaveantibodiesinCSFB?ttcheretal.,=>notusefultodifferentiatebetweenFIPandotherdiseases 貓feline infection(FIV)feline infectionfelineinfectiousperitonitisfeline infectionfeline infectionfelinepanleucopeniaDiagnosticOptionsFCV detection直 viralDNA/RNAviralprotein

的cell-bound indirectdetectionof cellularimmunityDiagnosticOptionsFCVdiagnostictestof viralRNA(PCR)inoropharyngeal口咽部棉拭 RNA（PCR）檢 extremelyhighprevalenceofchronic長期帶毒者有極高的流行strain 毒株多GeneralizedSystemic Syndrome ?alternativesdetectionof 病灶 測 分DiagnosticOptionsFCVdiagnostictestof viralRNA(PCR)inoropharyngeal口咽部棉拭 RNA（PCR）檢 extremelyhighprevalenceofchronic長期帶毒者有極高的流行strain 毒株多GeneralizedSystemic Syndrome ?alternativesdetectionof 病灶 測 分PrevalenceofFCVin歐洲FCV流行 多中心研Helpsetal.,prevalenceofFCVinmulti-cat多貓家庭FCV流行incatsincatterieswit