ABSTRACT-AmericanThoracicSociety摘要美國胸科協(xié)會

Title,Abstract,BackgroundandSignificanceBrucePittDepartmentEnvironmentaland OccupationalHealthUniversityPittsburghGraduateSchoolPublicHealth

SurvivalSkills& EthicsProgramBethFisherandMikeZigmundOutline,Write,andEditRememberto

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competingorrevised-sametitleACCURATEstatementoflongtermgoalsINCLUDEkeywordsApplicationPHSGrant09/04TITLE(FAQs)WhatifIdon’tconform?Howdoesmytitleaffectmyassignment?Whatmakesagoodtitle?TITLE(example)PhysiologyofthepulmonarycirculationNitricoxideandpulmonaryhypertensionPharmacologyofsodiumnitriteforpulmonaryhypertensioninsicklecelldiseaseTITLE(example)HostdefenseandlungbiologyMacrophagesandfungalinfectionoflungDectin-1,alveolarmacrophagesandImmunityAgainstPneumocystisCariniiinMiceABSTRACTPurpose:succinctlydescribeentireproposalImplications: a)grantreferralprocess(initialstudy sectionandinstituteassignments) b)significantsourceofinformationfor studysectionmembersnotassigned c)sourcereviewerassignmentABSTRACTHalf(confinedtodesignatedspace)Condensedmodifiedversionspecificaims:Essenceofspecificaims(exactlysamewordingfromsectionAforaims)FewsentencesonhealthrelatednessSignificanceintermsoflongtermgoals./sanders/htmlABSTRACT-SUGGESTIONSModifyspecificaims(SectionA)andwriteabstractlastUseentirespaceallottedandbecompleteConsiderimpactofdispersalofabstract: annualreportsandwebsite readilyaccessedbycolleagues, administratorsandlaypublic

Magnesium(Mg)deficiencymayplayanimportantroleinpathogenesisofenhancedvascularreactivityinhypertension.TheoverallhypothesistobeevaluatedisthatMgdeficiencycausedbyglucoseintolerance,insulinresistanceorotherfactorsinhypertensivesleadstoincreasedvasomotortoneviaalteredreleaseofvasoactivecyclooxygenaseandlipoxygenaseproductsofarachidonicacidandenhancedangiotensinII(AII)action.ToevaluatetheeffectsofMgdeficiencyinnormalsubjects,wewillinducetheconditionbyadministrationofalowMgdiet.VascularandadrenalsensitivitytoAII,plateletaggregationandeicosanoidlevelswillbestudiedpriortoandafterMgdeficiencyisestablished.SinceevidencesuggestsMgdeficiencycanmodulateinsulinaction,theeffectofthisdeficiencyonglucosetolerancewillalsobestudied.InanotherprojecttheeffectofinsulinonintracellularMglevelswillbestudiedusinganewfura2Mgdyetechnique.Thesestudieswillbeperformedingroupsofsubjectswithvariedbloodpressureandinsulinlevels.AlsotheeffectsofacuteintravenousandchronicoralMgloadingontheaboveparameterswillbestudiedinsimilarsubjectgroups.WewilldirectlystudytheeffectofMgonAII,insulinandinsulinlikegrowthfactoractioninisolatedandculturedadrenalglomerulosacells.ConcentrationofMgwillbevariedandsignaltransductionandsteroidogeniceffectswillbeevaluate.Thesestudieswillprovideinsightintomechamismsimportanttothepathogenesisofalteredvascularreactivityofsubjectswithhypertensionorhyperinsulinemia.

Magnesium(Mg)deficiencymayplayanimportantroleinpathogenesisofenhancedvascularreactivityinhypertension.TheoverallHYPOTHESIStobeevaluatedisthatMgdeficiencycausedbyglucoseintolerance,insulinresistanceorotherfactorsinhypertensivesleadstoincreasedvasomotortoneviaalteredreleaseofvasoactivecyclooxygenaseandlipoxygenaseproductsofarachidonicacidandenhancedangiotensinII(AII)action.

SpecificAims:(1)

DeterminetheeffectsoflowMgonvascularandadrenalsensitivitytoAII(plateletaggregationandeicosanoidlevels,glucosetolerance);(2)determinetheeffectofinsulinonintraceullarMglevels(fura2Mgdyetechnique).Thesestudieswillbeperformedingroupsofsubjectswithvariedbloodpressureandinsulinlevels;(3)determinetheeffectofacutei.v.andchronicoralMgloadingontheaboveparameters;(4)determinethesignaltransductionandsteroidogeniceffectsofMgandAII,insulinandinsulin-likegrowthfactoractioninisolatedandculturedadrenalglomerulosacells.

Significance:Thesestudieswillprovideinsightintomechamismsimportanttothepathogenesisofalteredvascularreactivityofsubjectswithhypertensionorhyperinsulinemia.

ABSTRACTCHECKLISTStaywithinspacewithunderstandablelanguageStatehypothesis,specificaims,significanceImportance,innovativenessExcludeconfidential,proprietaryinfoliteraturereviewrationaleforprojectstateofthefieldB.Background&Significancebeselectivedealw/contradictionsincludeownworkincludereviewer’sworkcitesourcesintext:

…waseffective(Jones&Smith,1996)inbiblio:JonesR,SmithM(1996)Effectofstressonethicalbehavior,Ethics,3:23-47.literaturereviewrationaleforprojectstateofthefieldyourproposalinnovationcontributiontofieldB.Background&SignificancebuildenthusiasmBACKGROUNDANDSIGNIFICANCE-PURPOSE3pageopportunitytodemonstratescholarshipbyconcisely a)statingproblemtobeinvestigated andrationaleforproposal b)placingproposalwithincurrentstate ofknowledge–notliteraturereview c)summarizingpotentialnew informationtobegleanedBACKGROUNDANDSIGNIFICANCE-CONTENTCombinationofliteraturecitations,referraltoownworkandthoughtfulschemacover: rationaleforproposal stateofexistingknowledgegapsthatprojectwillfillBACKGROUNDANDSIGNIFICANCE-SUGGESTIONSMakecompellingcase(importanceoftopicandresearchquestions,qualificationsofinvestigators)EstablishfamiliarityandauthorityinfieldCitations(contemporary,correct)Stressinnovations,newtechnologiesandimpactonrelatedfieldsofresearchBACKGROUNDANDSIGNIFICANCE-SUGGESTIONSCriticalanalysisofworkofothers–notnegativebutratherscholarlyrecognitionofcontributionsofotherscientistswhoseworkyoursbuildsonGapsinourunderstanding:explicitlystatewhatinformationismissingandhowyourworkwilladdresstheseissuesEmphasizesignificanceofproposal:assumeallreviewersagreeonimportanceofgeneralproblemandthusfocusonimpactofyourresearchonissueinquestion.GeneralorganizationuseheadingsfrequentlySymptomsImpactontheindividualEpilepsyGeneralorganizationwriteinparagraphs1majorideaperparagraphtopicsentencesinitialparagraphsofsectionmostimportantGeneralorganizationmakeiteasytofindkeypointsboldfacecrossreferencesomeredundancyAppearanceAppearanceselectgoodtypefaceAppearanceselectgoodtypefacegoodTimesRoman CenturySchoolbook

Appearanceselectgoodtypefacegoodavoid!TimesRoman courierCenturySchoolbook

Appearanceselectgoodtypefacegoodavoid!TimesRoman courierCenturySchoolbook

size>11pt(recommend11.5)AppearanceletyourtextindentparagraphsskiplinebetweenparagraphsbreatheBackgroundSignificance-ChecklistWrittenclearlyandnontechnically?ShowhowresearchinnovativeWhyworthfundingHowincreaseknowledgeIncludebackgroundinformationLiteraturerevealunderstandingGaps,discrepanciesorroadblocksinfieldAcknowledgementsOgden&GoldbergResearchProposals:AGuidetoSuccessMiner&GriffithProposalPlanning&WritingmanyProgramOfficers

staffBethFischerMichaelZigmondMaryDiBernardoMaureenGearysupportUniversityofPittsburghNSFNIHSurvivalSkills&EthicsProgramSamplereviewcriteria

NIHSignificanceApproachInnovationInvestigatorEnvironmentREFERENCESBauerDG.The“HowTo〞GrantsManual:SuccessfulGrantseekingTechniquesforObtainingPublicandPrivateGrants.3rdedition.Phoenix:OryxPress.1995.BowmanJP&BranchawBP.HowtoWriteProposalsThatProduce.Phoenix,AZ:OryxPress,1992.BrownLG.DemystifyingGrantseeking:WhatyouReallyNeedtoDoToGetGrants.SanFrancisco:Jossey-Bass,2001.BrowningB.GrantWritingforDummies.IDGBooksWorldwide,Inc.,2001.BurkeJ.I’llGrantYouThat:AStep-by-StepGuidetoFindingFunds,DesigningWinningProjects,andWritingPowerfulGrantProposals.Portsmouth,NH:Heinman,2000CarlsonM.WinningGrantsStepByStep:SupportCentersofAmerica’sCompleteWorkbookforPlanning,Developing,andWritingSuccessfulProposals.SanFrancisco:Jossey-Bass,1995.CarterNewC&QuickJA.Grantseeker’sToolkit:AComprehensiveGuidetoFindingFunding.NY:Wiley,1998.ColeySM&ScheinbergCA.ProposalWriting.NewburyPark,CA:SagePublications,1990.REFERENCES(CONT)DavitzJRDavitzLL.EvaluatingResearchProposals:AGuidefortheBehavioralSciences.UpperSaddleRiver,NJ:PrenticeHall,1996.FergusonJDrake-MajorLGershowitzMV.TheGrantseeker’sAnswerbook:FundraisingExpertsRespondtotheMostCommonlyAskedQuestions,2ndedition.Gaithersburg,MD:AspenPubs,1999.GoldenSL.SecretsofSuccessfulGrantsmanship:AGuerrillaGuidetoRaisingMoney.SanFrancisco,CA:Jossey-Bass,1997.IllesJ.