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WatersUPLCTroubleshootingTraining

AgendaACQUITYUPLCFamily：UPLC&H-ClassWatersUPLCSystemUPLCSolventManagement

(BSM)UPLCSampleManagement

(SM)WatersACQUITY

H-ClassSystemQuaternarySolventManager(QSM)SampleManager-FlowThroughNeedle(SM-FTN)WatersUPLCDetectorTroubleshootingTrainingWatersUPLCPDAandPDAeλTUVFLRELSDOpenDiscussion

ACQUITYUPLCFamilyACQUITYUPLCSystemACQUITYUPLCSystemDetectors:Opticaland/orMassSpecTunableUVorPhotodiodeArrayOptimizedflowcellforUPLCHighspeeddatasamplingColumnManager:InnovativepivotdesignPositionscolumntodetectorE-CordconnectionSampleManager:LowdispersionXYZZ’FormatFastcycletimesLowcarryoverPlatesand/orvialsOptionalSampleOrganizerBinarySolventManager:HighpressureblendingBinarygradientsFoursolventchoicesOn-linedegassingLowdispersiondesignUPLCpressurecapabilitiesSampleOrganizer:

(option)Expandscapacity(22/15/8)ShuttlesplatefeedHeated/chilledSystemConsiderations:SmallFootprintRedesignedtubingandfittingsConsolidatedwastemanagementIntegratedsystemdiagnosticsConnectionsInsightremotediagnosticsA1A2B1B2WasteFluidics

andConnectionsTubingmanagementChromatographicsolvents:A1/A2,B1/B2TwoSampleManagerwashsolventsSealWashCommonfittingsandtoolsforusersColumninlets/outletsandfittingsFingertight,?turn(use1/4”wrench)Dedicatedtubing3piececompressionscrewandferruleSmallfootprint–13?”wide24.25”withSampleOrganizerUPLC

SolventManagerACQUITYUPLCSystem

SolventDelivery

15KpsiperformanceAccurateandpreciseflowratesAccurateandprecisecompositionLowdelayvolumeforgradientapplications<120μLSystemVolumeLongtermreliabilityBinarySolventManagerComponentsFilter/Mixer/TeeAssembly“A”PumpAccumulator“A”PumpSolventSelectValve“B”PumpSolventSelectValve“A”PumpPrimary“B”PumpPrimary“B”PumpAccumulatorSixChannelDegasserIntelligentIntakeCheckValvesACQUITYUPLCSystem

FromSerialtoParallelFlowTwoindependentlydrivenprimaryandaccumulatorpistondrivesSeparatedrivemotorsTwotransducersTwoinletcheckvalvesFourSolventsCapabilityA1orA2andB1orB2TwosolventspergradientSixChannelDegasser4chromatographiceluents2solventsforSampleManagerStrong&weakwash“B”“A”B2B1A2SolventA1BinarySolventManager

InteractiveDisplayRecommendedSolventsListACQUITYUPLCSystemrecommendedSolvents

Methanol Methanol/watermixtures Water Acetonitrile/watermixtures Acetonitrile(ACN) Isopropanol(IPA)Samplediluents(inadditiontothesolventslistedabove)

Dimethylsulfoxide(DMSO) Dimethylformamide(DMF)Additives/Modifiers

0.2%formicacid 0.1%trifluoroaceticacid(TFA) 0.1%triethylamine(TEA) 0.1%hexafluorobutericacid 10mMphosphatebuffer 10mMammoniumbicarbonate 50mMammoniumhydroxide 50mMammoniumacetate 0.1%Ethylenediaminetetraaceticacid(EDTA)Cleaners

Phosphoricacid(=30%) Sodiumhydroxide(=1M)ACQUITYUPLCSystemnon-recommendedSolvents

Methylene-Chloride Chloroform Strongacids>5% EthylAcetate Toluene Chlorinatedsolvents:(Trichlorobenzene)

UPLC

SampleManagerSampleManagerFluidicsTray Pressure TransducerValveManifold

DriveGears

WashSyringesWeakWashStrongWashMeteringSyringeDriveAssemblyMeteringSyringeValve3WayRotaryValveMeteringSyringeAssemblySampleSyringe50uLvolumePN410001348100μLvolumePN700002570250μLvolumePN410001347forusewith50μLloopColumnHeatingCompartmentACQUITYIn-LineFilter19ColumnIn-LineFilterAssemblyColumnIn-LineFilterAssemblyColumnHeatingSampleManagerFlowPathAvailableLoopsDescription P/N Status 1μLLoop 430001516 Optional2μLLoop 430001264 Optional5μLLoop 430001233 Optional10μLLoop 430001326 Standard20μLLoop 430001320 Optional50μLLoop 430001325 Optional100μLLoop 170001792 (Kit)Optional250μLLoop 176001780 (Kit)OptionalVolumeConfigurationMenuBeforerunningtheCharacterizeNeedleandLoopVolumefunction,makesurethepropervolumesareconfiguredintheVolumesConfigurationmenu.TheabovevaluesarestandardforcurrentlyshippingACQUITYsystem.RecommendedInjectionVolumes,

LoopSizesandInjectionModesLoopVolume(uL)PartialLoopw/NeedleOverfillModeRecommendedsampleinjectionvolumerange(uL)PartialLoop(PressureAssistMode)Recommendedsampleinjectionvolumerange(uL)FullLoopModeRecommendedsampleinjectionvolume(uL0Automaticoverfillfactor1uL0.2to0.75NotRecommended1.05.82uL0.4to1.5NotRecommended2.05.65uL1.0to3.8NotRecommended5.0510uL2.0to7.52.0to5.010.0420uL4.0to15.04.0to10.020.0350uL10.0to37.510.0to25.050.02100uL20.0to75.020.0to50.0100.02250uL40.0to150.040.0to125.0250.01.5RecommendedPLNOinjectionvolume20%to75%ofloopvolumeRecommendedPartialLoopInjection20%to50%ofloopvolumeACQUITYH-ClassSystemQSMQuaternarySolventManagerSM-FTNSampleManager-FlowThroughNeedleCH-AHighTemperatureColumnHeaterActivePre-HeatingDetectorsTUV,PDAe

PDA,FLR,ELSDTheACQUITYH-ClassSystemconsistsofthefollowing:QuaternarySolventManager(QSM)FoursolventlowpressuregradientmixingSupportsUPLCPressuresLowgradientdelayvolumeSM-FTN(SampleManager-FlowThroughNeedle)Oneinjectionmode-directinjecttypeMuchsimpler-JustspecifyinjectionvolumeExcellentrecoveryandprecisionCH-AHightemperaturecolumnheaterwithactivepre-heatingACQUITYH-ClassSystemQSMFluidics-FrontViewPrimaryHeadAccumulatorHeadVentValve100ulMixerGradientProportioningValvePairsLowPressureManifoldDegasserChambersSealWashPumpQSMConsoleTopLevelMenuACQUITYH-ClassSystemSMDIFrontPanelFluidicsMeteringSyringeValveCheckValveandUnionInjectValveWashSolventInletLine(notdegassed)LeakSensorConnectionsSampleMeteringSyringeSDMIInjectValveConnectionsToCheckValveToCH-AandColumnStationarySideTubeFromPumpToPressureTransducerToSampleNeedleSampleCompartmentPowerandStatusLEDsPlateSelectorSwitchRotaryTray(PlateHolder)MagneticDoorSensorPowerSwitchSM-FTNInteractiveDisplayMenuCH-AImprovedDoorGasketAddedTubingGasket(topandbottom)For3rdpartyMassSpecatleftsideofACQUITYStack.Allowsuseofexist