板藍根有效成分的提取分離及含量測定

板藍根有效成分的提取分離及含量測定一、本文概述Overviewofthisarticle板藍根，作為一種常見的中藥材，具有清熱解毒、涼血利咽等多重功效，被廣泛應用于中醫(yī)藥臨床實踐中。隨著現代科學技術的發(fā)展，對板藍根有效成分的深入研究成為了提高其臨床應用效果和拓展其應用范圍的關鍵。本文旨在探討板藍根有效成分的提取分離技術，以及含量測定的方法，以期為板藍根的開發(fā)利用提供更為科學、精準的理論依據。Banlangen,asacommontraditionalChinesemedicine,hasmultipleeffectssuchasclearingheatanddetoxifying,coolingbloodandpromotingswallowing,andiswidelyusedinclinicalpracticeoftraditionalChinesemedicine.Withthedevelopmentofmodernscienceandtechnology,in-depthresearchontheeffectiveingredientsofBanlangenhasbecomethekeytoimprovingitsclinicalapplicationeffectandexpandingitsapplicationscope.ThisarticleaimstoexploretheextractionandseparationtechniquesofactiveingredientsinBanlangen,aswellasthemethodsforcontentdetermination,inordertoprovidemorescientificandaccuratetheoreticalbasisforthedevelopmentandutilizationofBanlangen.本文將首先概述板藍根有效成分的提取分離過程，包括提取方法的選擇、提取條件的優(yōu)化以及分離純化技術的應用等方面。隨后，本文將詳細介紹板藍根中主要有效成分的含量測定方法，包括紫外可見分光光度法、高效液相色譜法、氣相色譜法等現代分析技術。本文還將對板藍根有效成分的提取分離及含量測定的研究進展進行綜述，以期為后續(xù)研究提供參考和借鑒。ThisarticlewillfirstprovideanoverviewoftheextractionandseparationprocessofeffectivecomponentsfromBanlangen,includingtheselectionofextractionmethods,optimizationofextractionconditions,andapplicationofseparationandpurificationtechniques.Subsequently,thisarticlewillprovideadetailedintroductiontothemethodsfordeterminingthecontentofthemainactiveingredientsinBanlangen,includingmodernanalyticaltechniquessuchasUVvisiblespectrophotometry,high-performanceliquidchromatography,andgaschromatography.Thisarticlewillalsoprovideareviewoftheresearchprogressontheextraction,separation,andcontentdeterminationofactiveingredientsinBanlangen,inordertoprovidereferenceandinspirationforsubsequentresearch.通過本文的研究，我們期望能夠為板藍根有效成分的深入研究和應用開發(fā)提供有益的參考，推動板藍根在現代醫(yī)藥領域的應用和發(fā)展。Throughtheresearchinthisarticle,wehopetoprovideusefulreferencesforthein-depthstudyandapplicationdevelopmentoftheactiveingredientsofBanlangen,andpromotetheapplicationanddevelopmentofBanlangeninthefieldofmodernmedicine.二、板藍根有效成分的提取方法ExtractionmethodofactiveingredientsfromBanlangen板藍根作為一種具有廣泛藥用價值的中藥材，其有效成分的提取方法對于后續(xù)的分離和含量測定至關重要。常見的板藍根有效成分提取方法主要有以下幾種：AsaChinesemedicinalherbwithextensivemedicinalvalue,theextractionmethodofitseffectivecomponentsiscrucialforsubsequentseparationandcontentdetermination.ThecommonmethodsforextractingeffectiveingredientsfromBanlangenmainlyincludethefollowing:溶劑提取法：這是最常用的提取方法，主要利用不同的溶劑對板藍根中的有效成分進行提取。常用的溶劑包括水、乙醇、甲醇等。根據有效成分的溶解性，選擇合適的溶劑進行浸泡、回流或滲漉，從而提取出板藍根中的有效成分。Solventextractionmethod:Thisisthemostcommonlyusedextractionmethod,whichmainlyusesdifferentsolventstoextracttheeffectivecomponentsinBanlangen.Commonsolventsincludewater,ethanol,methanol,etc.Basedonthesolubilityoftheactiveingredients,chooseasuitablesolventforsoaking,reflux,orpercolationtoextracttheactiveingredientsfromBanlangen.超聲波輔助提取法：這種方法利用超聲波的空化效應、機械效應和熱效應，加速板藍根中有效成分的溶解和擴散，從而提高提取效率。超聲波輔助提取法具有操作簡便、提取時間短、提取效率高等優(yōu)點。Ultrasonicassistedextractionmethod:Thismethodutilizesthecavitationeffect,mechanicaleffect,andthermaleffectofultrasoundtoacceleratethedissolutionanddiffusionofeffectivecomponentsinBanlangen,therebyimprovingextractionefficiency.Theultrasonicassistedextractionmethodhastheadvantagesofsimpleoperation,shortextractiontime,andhighextractionefficiency.微波輔助提取法：微波輔助提取法利用微波的熱效應和非熱效應，對板藍根中的有效成分進行快速、高效的提取。這種方法具有提取時間短、提取效率高、溶劑消耗少等優(yōu)點。Microwaveassistedextractionmethod:MicrowaveassistedextractionmethodutilizesthethermalandnonthermaleffectsofmicrowavetoquicklyandefficientlyextracttheeffectivecomponentsinBanlangen.Thismethodhastheadvantagesofshortextractiontime,highextractionefficiency,andlowsolventconsumption.超臨界流體萃取法：超臨界流體萃取法是一種新型的提取技術，利用超臨界流體（如二氧化碳）的特殊性質，對板藍根中的有效成分進行高效、環(huán)保的提取。這種方法具有提取效率高、提取溫度低、不破壞有效成分等優(yōu)點。Supercriticalfluidextractionmethod:Supercriticalfluidextractionisanewtypeofextractiontechnologythatutilizesthespecialpropertiesofsupercriticalfluids(suchascarbondioxide)toefficientlyandenvironmentallyextracttheactiveingredientsinBanlangen.Thismethodhastheadvantagesofhighextractionefficiency,lowextractiontemperature,andnodamagetoeffectivecomponents.在選擇提取方法時，需要根據板藍根中有效成分的性質、提取效率、設備條件等因素進行綜合考慮。為了獲得最佳的提取效果，往往需要對提取方法進行優(yōu)化，如調整溶劑種類、提取溫度、提取時間等參數。在提取過程中，還需注意操作規(guī)范，避免有效成分的損失和污染。Whenselectingtheextractionmethod,itisnecessarytocomprehensivelyconsiderfactorssuchasthepropertiesoftheactiveingredientsinBanlangen,extractionefficiency,andequipmentconditions.Inordertoachievethebestextractioneffect,itisoftennecessarytooptimizetheextractionmethod,suchasadjustingparameterssuchassolventtype,extractiontemperature,andextractiontime.Duringtheextractionprocess,attentionshouldalsobepaidtooperatingprocedurestoavoidlossandcontaminationofactiveingredients.三、板藍根有效成分的分離技術SeparationtechnologyofactiveingredientsinBanlangen板藍根作為一種傳統(tǒng)中藥材，其有效成分的提取和分離對于其藥效的研究和利用至關重要。有效的分離技術不僅可以提高目標成分的純度，還能為后續(xù)的含量測定和藥物研發(fā)提供可靠的基礎。AsatraditionalChinesemedicinalherb,theextractionandseparationofeffectivecomponentsfromBanlangenarecrucialforthestudyandutilizationofitspharmacologicaleffects.Effectiveseparationtechniquescannotonlyimprovethepurityoftargetcomponents,butalsoprovideareliablefoundationforsubsequentcontentdeterminationanddrugdevelopment.在板藍根有效成分的分離過程中，常用的技術包括液液萃取、色譜分離和薄層色譜等。液液萃取是利用不同溶劑對目標成分的選擇性溶解能力，通過多次萃取將目標成分從原料中分離出來。色譜分離則是一種基于物質在固定相和移動相之間分配系數的差異，通過不斷改變流動相的成分和比例，實現目標成分的逐步分離。薄層色譜則是一種簡便快速的分離方法，通過在薄層板上涂布固定相，然后將待分離樣品點在薄層上，利用毛細作用使流動相沿薄層上升，從而實現不同成分的分離。IntheseparationprocessofactiveingredientsinBanlangen,commonlyusedtechniquesincludeliquid-liquidextraction,chromatographicseparation,andthin-layerchromatography.Liquidliquidextractionistheprocessofutilizingtheselectivedissolutionabilityofdifferentsolventstoseparatethetargetcomponentfromtherawmaterialthroughmultipleextractions.Chromatographicseparationisagradualseparationoftargetcomponentsbasedonthedifferenceindistributioncoefficientsbetweenstationaryandmobilephases,bycontinuouslychangingthecompositionandproportionofthemobilephase.Thinlayerchromatographyisasimpleandfastseparationmethod,whichinvolvescoatingastationaryphaseonathinlayerplate,thenplacingthesampletobeseparatedonthethinlayer,andusingcapillaryactiontoraisethemobilephasealongthethinlayer,thusachievingtheseparationofdifferentcomponents.在分離過程中，選擇合適的分離技術至關重要。對于板藍根中的不同有效成分，需要根據其化學性質和溶解性選擇合適的分離技術。例如，對于極性較大的成分，可以選擇液液萃取中的親水性溶劑進行提取；對于極性較小的成分，則可以選擇色譜分離中的反相色譜進行分離。Choosingtheappropriateseparationtechnologyiscrucialintheseparationprocess.ForthedifferenteffectivecomponentsinBanlangen,itisnecessarytochooseappropriateseparationtechniquesbasedontheirchemicalpropertiesandsolubility.Forexample,forcomponentswithhighpolarity,hydrophilicsolventsinliquid-liquidextractioncanbechosenforextraction;Forcomponentswithlowerpolarity,reversephasechromatographyinchromatographicseparationcanbechosenforseparation.除了選擇合適的分離技術外，分離過程中的操作條件也對分離效果產生重要影響。例如，在液液萃取中，萃取劑的種類、用量和萃取次數等因素都會影響目標成分的提取率；在色譜分離中，流動相的成分、比例和流速等因素則會影響目標成分的分離效果和純度。Inadditiontoselectingappropriateseparationtechniques,theoperatingconditionsduringtheseparationprocessalsohaveasignificantimpactontheseparationeffect.Forexample,inliquid-liquidextraction,factorssuchasthetype,amount,andextractionfrequencyoftheextractantcanaffecttheextractionrateofthetargetcomponent;Inchromatographicseparation,factorssuchasthecomposition,proportion,andflowrateofthemobilephasecanaffecttheseparationefficiencyandpurityofthetargetcomponent.板藍根有效成分的分離技術對于其藥效研究和利用具有重要意義。在實際操作中，需要根據目標成分的性質選擇合適的分離技術，并優(yōu)化操作條件以獲得最佳的分離效果。TheseparationtechnologyofactiveingredientsinBanlangenisofgreatsignificanceforitspharmacologicalresearchandutilization.Inpracticaloperation,itisnecessarytoselectappropriateseparationtechniquesbasedonthepropertiesofthetargetcomponentsandoptimizeoperatingconditionstoachievethebestseparationeffect.四、板藍根有效成分的含量測定方法MethodfordeterminingthecontentofactiveingredientsinBanlangen板藍根作為一種重要的中藥材，其有效成分的準確含量測定對于確保其質量和療效至關重要。目前，常用的板藍根有效成分含量測定方法主要包括高效液相色譜法（HPLC）、薄層色譜法（TLC）以及紫外-可見分光光度法（UV-Vis）等。AsanimportanttraditionalChinesemedicine,accuratedeterminationoftheeffectiveingredientsinBanlangeniscrucialforensuringitsqualityandefficacy.Atpresent,thecommonlyusedmethodsfordeterminingthecontentofactiveingredientsinBanlangenmainlyincludehigh-performanceliquidchromatography(HPLC),thin-layerchromatography(TLC),andUVVisspectrophotometry.高效液相色譜法（HPLC）：此方法是目前最常用的含量測定手段之一。通過色譜柱對板藍根中的有效成分進行分離，然后用檢測器測定其峰面積或峰高，與標準品進行比較，從而計算出樣品中有效成分的含量。該方法具有分離效果好、靈敏度高、重現性好等優(yōu)點，是板藍根有效成分含量測定的首選方法。Highperformanceliquidchromatography(HPLC):Thismethodiscurrentlyoneofthemostcommonlyusedmethodsforcontentdetermination.SeparatetheeffectivecomponentsinBanlangenusingachromatographiccolumn,andthenmeasuretheirpeakareaorpeakheightusingadetector.Comparethemwiththestandardsampletocalculatethecontentoftheeffectivecomponentsinthesample.Thismethodhastheadvantagesofgoodseparationeffect,highsensitivity,andgoodreproducibility,andisthepreferredmethodfordeterminingthecontentofactiveingredientsinBanlangen.薄層色譜法（TLC）：TLC是一種簡便、快速的定性、半定量分析方法。通過在不同溶劑系統(tǒng)中展開板藍根樣品，觀察其在薄層板上的色譜行為，與標準品進行比較，可以初步判斷樣品中有效成分的種類和相對含量。但該方法精度較低，適用于大批量樣品的初步篩選。Thinlayerchromatography(TLC):TLCisasimpleandfastqualitativeandsemiquantitativeanalysismethod.ByunfoldingBanlangensamplesindifferentsolventsystemsandobservingtheirchromatographicbehavioronthinlayerplates,andcomparingthemwithstandardsamples,thetypesandrelativecontentsofeffectiveingredientsinthesamplescanbepreliminarilydetermined.However,thismethodhaslowaccuracyandissuitableforpreliminaryscreeningoflarge-scalesamples.紫外-可見分光光度法（UV-Vis）：該方法基于板藍根有效成分在特定波長下具有吸收峰的特性，通過測定樣品在特定波長下的吸光度，與標準品進行比較，計算出樣品中有效成分的含量。該方法操作簡便、快速，適用于大批量樣品的含量測定。但需要注意的是，該方法只能測定具有特征吸收峰的成分，對于無特征吸收峰或吸收峰重疊的成分則無法準確測定。UVVisspectrophotometry:ThismethodisbasedonthecharacteristicofabsorptionpeaksofactiveingredientsinBanlangenatspecificwavelengths.Bymeasuringtheabsorbanceofthesampleataspecificwavelengthandcomparingitwiththestandard,thecontentofactiveingredientsinthesampleiscalculated.Thismethodiseasytooperate,fast,andsuitableforthedeterminationofcontentinlargebatchesofsamples.However,itshouldbenotedthatthismethodcanonlydeterminecomponentswithcharacteristicabsorptionpeaks,andcannotaccuratelydeterminecomponentswithoutcharacteristicabsorptionpeaksoroverlappingabsorptionpeaks.除了以上三種方法外，還有一些其他方法如氣相色譜法（GC）、氣相色譜-質譜聯(lián)用法（GC-MS）等也可用于板藍根有效成分的含量測定。但由于這些方法操作復雜、成本較高，因此在實際應用中較少使用。Inadditiontotheabovethreemethods,therearealsosomeothermethodssuchasgaschromatography(GC)andgaschromatography-massspectrometry(GC-MS)thatcanbeusedforthedeterminationofthecontentofactiveingredientsinRadixIsatidis.However,duetothecomplexityandhighcostofthesemethods,theyarerarelyusedinpracticalapplications.在進行板藍根有效成分含量測定時，應根據具體成分的性質和實驗條件選擇合適的方法。為確保結果的準確性和可靠性，還應嚴格按照標準操作程序進行實驗，并對實驗數據進行合理處理和分析。WhendeterminingthecontentofactiveingredientsinBanlangen,appropriatemethodsshouldbeselectedbasedonthepropertiesofspecificcomponentsandexperimentalconditions.Toensuretheaccuracyandreliabilityoftheresults,experimentsshouldalsobeconductedstrictlyinaccordancewithstandardoperatingprocedures,andtheexperimentaldatashouldbeprocessedandanalyzedreasonably.五、實驗研究與結果分析Experimentalresearchandresultanalysis實驗所用的板藍根樣品購自正規(guī)藥店，經過嚴格的鑒定確保其質量。使用的化學試劑均為分析純，實驗設備包括高效液相色譜儀、紫外可見分光光度計、旋轉蒸發(fā)儀、真空干燥箱等。ThesampleofBanlangenusedintheexperimentwaspurchasedfromalegitimatepharmacyanditsqualitywasensuredthroughstrictidentification.Thechemicalreagentsusedareallanalyticalgrade,andtheexperimentalequipmentincludeshigh-performanceliquidchromatography,UVvisiblespectrophotometer,rotaryevaporator,vacuumdryingoven,etc.板藍根有效成分的提取采用溶劑提取法，通過不斷優(yōu)化提取條件（如提取溶劑、溫度、時間等），得到最佳提取工藝。提取后的樣品經過濃縮、干燥，再通過硅膠柱色譜、制備型高效液相色譜等方法進行分離純化。TheextractionofactiveingredientsfromBanlangenadoptssolventextractionmethod,andtheoptimalextractionprocessisobtainedbycontinuouslyoptimizingextractionconditions(suchasextractionsolvent,temperature,time,etc.).Theextractedsampleisconcentrated,dried,andthenseparatedandpurifiedbymethodssuchassilicagelcolumnchromatographyandpreparativehigh-performanceliquidchromatography.含量測定采用高效液相色譜法，通過對比標準品色譜圖，確定樣品中有效成分的種類和含量。同時，還采用了紫外可見分光光度計對提取物的總黃酮含量進行了測定。Thecontentdeterminationiscarriedoutusinghigh-performanceliquidchromatography.Bycomparingthestandardcolorspectrum,thetypesandcontentsofeffectiveingredientsinthesamplearedetermined.Meanwhile,aUVvisiblespectrophotometerwasalsousedtodeterminethetotalflavonoidcontentoftheextract.經過實驗，我們成功提取并分離了板藍根中的多種有效成分，包括黃酮類化合物、生物堿等。高效液相色譜法測定結果顯示，這些成分在樣品中的含量較為豐富，且分離效果較好。Throughexperiments,wehavesuccessfullyextractedandisolatedvariouseffectivecomponentsfromBanlangen,includingflavonoids,alkaloids,etc.Theresultsofhigh-performanceliquidchromatographyshowedthatthesecomponentswereabundantinthesampleandhadgoodseparationeffects.通過對比不同提取條件下的提取效果，我們發(fā)現使用70%乙醇作為提取溶劑、在60℃下提取60分鐘，能得到最佳的提取效果。我們還發(fā)現提取時間對提取效果的影響較大，過長的提取時間會導致部分成分的降解。Bycomparingtheextractioneffectsunderdifferentextractionconditions,wefoundthatusing70%ethanolastheextractionsolventandextractingat60℃for60minutescanachievethebestextractioneffect.Wealsofoundthatextractiontimehasasignificantimpactontheextractioneffect,andexcessiveextractiontimecanleadtothedegradationofsomecomponents.含量測定結果表明，板藍根中黃酮類化合物的含量較高，占總提取物的30%以上。這一結果與文獻報道相符，進一步證實了板藍根的藥理作用與其黃酮類成分密切相關。ThecontentdeterminationresultsshowthatthecontentofflavonoidsinBanlangenisrelativelyhigh,accountingformorethan30%ofthetotalextract.Thisresultisconsistentwithliteraturereports,furtherconfirmingthatthepharmacologicaleffectsofBanlangenarecloselyrelatedtoitsflavonoidcomponents.本實驗成功提取并分離了板藍根中的有效成分，并建立了有效的含量測定方法。這為深入研究板藍根的藥理作用和開發(fā)新藥物提供了有價值的參考信息。實驗結果也為板藍根的質量控制提供了科學依據。ThisexperimentsuccessfullyextractedandseparatedtheeffectivecomponentsfromBanlangen,andestablishedaneffectivecontentdeterminationmethod.Thisprovidesvaluablereferenceinformationforin-depthresearchonthepharmacologicaleffectsofBanlangenandthedevelopmentofnewdrugs.TheexperimentalresultsalsoprovideascientificbasisforthequalitycontrolofBanlangen.六、板藍根有效成分的藥理作用及臨床應用PharmacologicaleffectsandclinicalapplicationsofactiveingredientsinBanlangen板藍根作為一種傳統(tǒng)中藥材，在中醫(yī)理論中早已被廣泛應用。近年來，隨著現代科學技術的進步，對板藍根有效成分的藥理作用和臨床應用進行了深入研究，進一步驗證了其在現代醫(yī)學中的價值和地位。Banlangen,asatraditionalChinesemedicinalherb,haslongbeenwidelyusedintraditionalChinesemedicinetheory.Inrecentyears,withtheadvancementofmodernscienceandtechnology,in-depthresearchhasbeenconductedonthepharmacologicaleffectsandclinicalapplicationsoftheactiveingredientsofBanlangen,furtherverifyingitsvalueandpositioninmodernmedicine.板藍根中的有效成分主要包括多種生物堿、黃酮類化合物等，這些成分具有抗病毒、抗菌、抗炎、抗氧化等多種藥理作用。在抗病毒方面，板藍根中的某些成分能夠有效抑制流感病毒、皰疹病毒等病毒的復制，對于預防和治療病毒性感冒、帶狀皰疹等疾病具有良好的效果。在抗菌方面，板藍根的有效成分對金黃色葡萄球菌、大腸桿菌等常見細菌也具有明顯的抑制作用，可用于輔助治療由這些細菌引起的感染。TheactiveingredientsinBanlangenmainlyincludevariousalkaloids,flavonoids,etc.Theseingredientshavevariouspharmacologicaleffectssuchasantiviral,antibacterial,anti-inflammatory,andantioxidant.Intermsofantiviraleffects,certaincomponentsinBanlangencaneffectivelyinhibitthereplicationofinfluenzavirus,herpesvirus,andotherviruses,andhavegoodeffectsinpreventingandtreatingviralcolds,herpeszoster,andotherdiseases.Intermsofantibacterialproperties,theactiveingredientsofBanlangenalsohaveasignificantinhibitoryeffectoncommonbacteriasuchasStaphylococcusaureusandEscherichiacoli,andcanbeusedasanadjuncttothetreatmentofinfectionscausedbythesebacteria.板藍根的有效成分還具有顯著的抗炎作用，能夠減輕炎癥反應，緩解腫脹、疼痛等癥狀，對于治療咽喉炎、扁桃體炎等炎癥性疾病有良好效果。同時，板藍根還具有抗氧化作用，能夠清除體內的自由基，保護細胞免受氧化損傷，對于預防和治療氧化應激相關的疾病具有積極意義。TheeffectiveingredientsofBanlangenalsohavesignificantanti-inflammatoryeffects,whichcanreduceinflammatoryreactions,alleviatesymptomssuchasswellingandpain,andhavegoodeffectsonthetreatmentofinflammatorydiseasessuchaspharyngitisandtonsillitis.Atthesametime,Banlangenalsohasantioxidanteffects,whichcaneliminatefreeradicalsinthebody,protectcellsfromoxidativedamage,andhavepositivesignificanceforthepreventionandtreatmentofoxidativestress-relateddiseases.在臨床應用方面，板藍根已被廣泛應用于感冒、流感、咽喉炎、扁桃體炎等疾病的治療。由于其抗病毒、抗菌、抗炎等多重作用，板藍根在這些疾病的治療中表現出良好的療效。隨著對板藍根藥理作用的深入研究，其在其他領域的臨床應用也在不斷拓展，如皮膚科、口腔科等。Intermsofclinicalapplication,Banlangenhasbeenwidelyusedinthetreatmentofdiseasessuchascolds,influenza,pharyngitis,andtonsillitis.Duetoitsmultipleeffectssuchasantiviral,antibacterial,andanti-inflammatory,Banlangenhasshowngoodtherapeuticeffectsinthetreatmentofthesediseases.Withthein-depthstudyofthepharmacologicaleffectsofBanlangen,itsclinicalapplicationsinotherfieldsarealsoconstantlyexpanding,suchasdermatologyanddentistry.板藍根的有效成分具有多種藥理作用，在臨床醫(yī)學中具有廣泛的應用前景。未來，隨著科學技術的不斷進步和臨床經驗的積累，板藍根的藥理作用和臨床應用將得到更深入的研究和拓展，為人類健康事業(yè)作出更大的貢獻。TheactiveingredientsofBanlangenhavevariouspharmacologicaleffectsandhavebroadapplicationprospectsinclinicalmedicine.Inthefuture,withthecontinuousprogressofscienceandtechnologyandtheaccumulationofclinicalexperience,thepharmacologicaleffectsandclinicalapplicationsofBanlangenwillbefurtherstudiedandexpanded,makinggreatercontributionstothecauseofhumanhealth.七、展望與建議Outlookandsuggestions隨著現代科學技術的發(fā)展，特別是生物技術和分析技術的不斷進步，對板藍根有效成分的研究已經取得了顯著的成果。然而，對于這一傳統(tǒng)中藥材的深度挖掘和應用，仍有許多工作需要做。Withthedevelopmentofmodernscienceandtechnology,especiallythecontinuousprogressofbiotechnologyandanalyticaltechnology,significantachievementshavebeenmadeinthestudyoftheactiveingredientsofBanlangen.However,thereisstillmuchworktobedoneinthedeepexcavationandappli