血液細(xì)胞分析儀原理及應(yīng)用

1、Principle and application of blood cell analyzer (血液細(xì)胞分析儀原理及應(yīng)用) Human physiological and pathological changes, often caused by changes in blood components, so that a timely understanding of the changes in the blood component, can provide a diagnosis and treatment of diseases of the important basis fo

2、r doctors. Red blood cell Biconcave disc Seedless Red blood cell diameter between 6 9 m, an average of 7.2 M. The number of red blood cells of the adult male was (4.6 6.2) * 1012 /L The number of red blood cells of the adult female was (5.4 4.2) * 1012 /L White blood cell Nuclear sphere The diameter

3、 is 7 25 M. The number of white blood cells in normal persons was (10 4) * 109 /L Platelet Both sides of the micro concave disk, when stimulated pseudopodia The normal platelet value (100 300) * 109 /L, diameter 2 4 m. Hemoglobin (hemoglobin or heme) Hemoglobin is present in red blood cells and is a

4、 kind of protein containing iron. Adult male is 120 160g/L Adult female is 110 150g/LMeasurement parameters of blood cell analyzer Red blood cell RBC White blood cell WBC Platelet PLT Hemoglobin HGB Red blood cell RBC White blood cell WBC Platelet PLT Hemoglobin HGB一、The development history of blood

5、 cell analyzer In 1956, the American scientist W.H.Coulter patented technology for the use of the resistance method to count the number of particles in the blood cell count to be successful. The principle is based on the nature of the non conduction of blood cells, the blood cell count and the volum

6、e of blood cells were detected by the blood cell count and volume measurement. The method is called the resistance method or the Kurt principle. 國外廠商： 日本SYSMEX 美國beckman-coulter 日本KOHDEN 美國DANAM 瑞典BOULE 美國BAYER 美國ABBOTT 國內(nèi)廠商： 南昌特康 南昌百特 深圳邁瑞 桂林百利特1. 庫爾特計數(shù)原理 庫爾特原理（coulter principle） ：二、血細(xì)胞計數(shù)檢測原理Coulte

7、r principle When the electrolyte is suspended in the electrolyte, the same volume of electrolyte is replaced by the electrolyte through the hole, and the electric resistance of the two electrode is changed, and the potential pulse is generated. The size and frequency of the pulse signal is proportio

8、nal to the size of the particles and the number of the particles.二、血細(xì)胞計數(shù)檢測原理儀器是如何進(jìn)行細(xì)胞分群的呢？ 儀器除給出細(xì)胞數(shù)據(jù)結(jié)果外，同時提供出細(xì)胞體積分布圖形，這些可以表示出細(xì)胞群體分布情況的圖形被稱為直方圖 它可以顯示出某一特定細(xì)胞群的平均細(xì)胞體積、細(xì)胞分布情況和是否存在明顯的異常細(xì)胞群 直方圖是由測量通過感應(yīng)區(qū)的每個細(xì)胞脈沖累積得到的，根據(jù)庫爾特原理可以在計數(shù)的同時進(jìn)行分析測量。 二、血細(xì)胞計數(shù)檢測原理Relationship between histogram and pulse signal二、血細(xì)胞計數(shù)檢測原

9、理三、Structure of ACT diff Coulter blood cell analyzer The volume and number of blood cells were measured by using the principle of Kurt resistance, and the hemoglobin was detected by using the principle of monochromatic light color, and the three classification of white blood cells was obtained accor

10、ding to the distribution of blood cell volume.。 35 to 50 samples per hour. WBC,RBC,HGB,PLT,LY,MO,GI三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu) Card slot Cards and reagents for the corresponding batch should be三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu) Counting software card Positive internalSuction needle Reaction tank Photoelect

11、ric tube Solenoid valve Scan tube Vacuum isolation chamber三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu) Reaction tank三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu) Right side Creeping pump Dilution liquid storage tank Needle Solenoid valve Filter Air pump三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu) Pinch valve三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu) Valve core Cont

12、rol board三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu)Instrument structureGas path: vacuum - pumping waste liquid, drying; compressed air - producing bubblesLiquid path: sample, hemolytic agent, diluent, waste liquid and cleaning liquidCircuit: control of gas path, counting and calculation.三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu)Piping working process:SampleWipe the needle into the external and drySample dilution such as WBC reaction tankThe two dilution sample was injected into the RBC reaction tankIn WBC reaction tank, the injection of hemolytic agentWBC count (control), PLT count, HGB count (photo