各種激發(fā)光和吸收光波長

1、各種激發(fā)光和吸收光波長現(xiàn)在發(fā)現(xiàn)一個更全的有關激發(fā)光和發(fā)射光的波長圖譜。這對CONFOCAL做多重染色時是必不可少的參考資料。全部文件請到這里下載！Excitation and Emission Wavelengths of FluorophoresTavi's "FluoroTable": Common fluorophores(from Zeiss Corporation website)FluorophoreAbsorptionEmission Other info1,5 IAEDANS3364901,8-ANS3724804-Methylumbellifero

2、ne3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine542568(5-TAMRA)5-FAM (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-540

3、5-ROX (carboxy-X-rhodamine)5786045675915-TAMRA548552(5-Carboxytetramethylrhodamine)5425686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471,474ABQ344445A

4、cid Fuchsin540630ACMA412, 430471,474(9-Amino-6-chloro-2-methoxyacridine)Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466Ph

5、otoproteinAFPs - AutoFluorescentProtein -(Quantum Biotechnologies)see sgGFP,sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531,532553, 554Alexa Fluor 546556,557572,573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Ale

6、xa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complexon530-560,580,580624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345425347444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346442350445Aminomethylcoumarin (AMCA

7、)345425347444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG?

8、 CBQCA465560ATTO-TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395BCECF (high pH)492,503520,528BCECF (low pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381,382, 445, 447, blue shifted GFP (Y66H)Blue Flu

9、orescent ProteinBFP/GFP FRET383448BlueFluorescentProteinBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505 Ratio Dye, Zn2+Blancophor FFG390470Blancophor SV370435BOBO?-1462481BOBO?-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy

10、 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504,505511,513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574B

11、odipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO?-1462481BO-PRO?-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529 Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532 Ratio Dye, Zn2+Calcein494517Calcein Blue373440Cal

12、cium Crimson588, 589611,615Calcium Green501,506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385,395, 437, 440,405445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue3774203

13、98423399Cascade Yellow399550400552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430,433, 474, 475, CyanFluorescent436, (453)476,Protein(501)CFP/YFP FRETChlorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Co

14、elenterazineCa2+Dye, (429)465Ca2+Dye,bioluminescencebioluminescence,native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+Dye,bioluminescenceCoelenterazine f(437)473Ca2+Dye,bioluminescenceCoelenterazine fcp452Ca2+Dye,bioluminescenceCoelenterazine h(437)464Ca2+Dye,bioluminescenceCoelenterazine hcp(

15、433)444Ca2+Dye,bioluminescenceCoelenterazine ip441Ca2+Dye,bioluminescenceCoelenterazine n(431)467Ca2+bioluminescenceDye,Coelenterazine O460575Coumarin Phalloidin387470C-phycocyanineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2489506Cy3.1 8554568Cy3.5581598Cy3514566552570554Cy5.1

16、 8649666Cy5.5675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453)475(501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520CellProliferationAssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI3

17、59461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH(DichlorodihydrofluoresceinDiacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488605498713DiA (4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate505535(DCFH)DiD - Lipophilic Tracer644665Li

18、pophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535Dil (DiIC18(3) Dinitrophenol549, 551349565DiO (DiOC18(3)484,487501,502DiR748780Lipophilic TracerDiR (DiIC18)750779DM-NERF (high pH) DNP497/510349540Ratio Dye, pHDopamine340490-520DsRed558583Red fluorescent proteinDTAF494520D

19、Y-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447EnhancedBlueFluorescent ProteinECFP436474EnhancedCyanFluorescent ProteinEGFP488, 498507, 516 EnhancedGreenFluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554,555Erythrosin ITC52

20、9555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617EuchrysinEukoLightEuropium (III) chloride430540EYFP513, 520527, 532 EnhancedYellowFluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Anti

21、body493517Flazo Orange375-530612Fluo-3480 ?506,520,527506Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red?(high pH)572657Fura Red?Fluo-3Fura-2, high calci

22、um335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation475509(wtGFP)GFP wild type, UV excitation (wtGFP) 395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347Filter sets installed on the Axiovert 200M:1) BrightfieldDIC:filter cube