Betaine-hydrochloride-Betaine-chloride-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEBetaine hydrochlorideCat. No.: HY-N0739CAS No.: 590-46-5Synonyms: Betaine chloride分式: CHClNO分量: 153.61作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) H2O : 43 mg/mL (279.93 m

2、M)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 6.5100 mL 32.5500 mL 65.0999 mL5 mM 1.3020 mL 6.5100 mL 13.0200 mL10 mM 0.6510 mL 3.2550 mL 6.5100 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Betaine hydrochloride許多物中發(fā)現(xiàn)的天然化合物，也 種可維

3、持正常 DNA 甲基化模式的活性甲基供體。體外研究 It is found that proliferation of HeLa cells is inhibited significantly upon exposure to increasing Betainehydrochloride levels with the MTT test (p5.0 mg/mL) significantly promotes the apoptosis of HeLa cells (p 1.1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemE體內(nèi)研究 Liver

4、-to-body weight ratio is attenuated by Betaine hydrochloride supplementation. When Betainehydrochloride is added to the alcohol-containing liquid diet, both hepatic triglyceride (TG) accumulation andliver injury are significantly reduced. Betaine hydrochloride supplementation attenuates epididymal f

5、at padmass loss induced by chronic alcohol feeding. Betaine hydrochloride supplementation prevents the depletionof S-adenosylhomocysteine (SAM) and reduces the increase in S-adenosylhomocysteine (SAH), therebyimproving the methylation state of the adipose tissue 2.PROTOCOLCell Assay 1 Cytotoxic effe

6、cts of Betaine hydrochloride are evaluated by MTT assays. Briefly, 5103 HeLa or MCF-10Acells/well are seeded in a 96-well plate and cultured overnight. Then, different concentrations of Betainehydrochloride (from 0 to 100 mg/mL) are added to each well, and the MTT assay is performed at 24, 48, 72and

7、 96 h after incubation. Cu/Zn-SOD activities are detected using the superoxide dismutase Assay Kitaccording to the manufacturers instructions 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Male C57BL/6 mice weighing 250.5 g are used in this st

8、udy. Animals are randomly assigned to four groupsAdministration 2 (n=6 to 8 per group) and pair-fed a modified Lieber-DeCarli alcohol (AF) or isocaloric maltose dextrin controlliquid diet (PF) for 5 weeks with a stepwise feeding procedure. The ethanol content starts at 30% of totalcalories and gradu

9、ally increases up to 36% in the last week. Betaine hydrochloride is administered as asupplement in the liquid diets (both PF and AF) at a concentration of 0.5% (w/v), simultaneously with thealcohol. All animals have access to diet and water ad libitum. At the end of the experiment, the mice arekille

10、d after being deprived of food for 4h, and the plasma, liver and epididymal fat pad samples are harvestedfor assays 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Guo Y, et al. Betaine Effects on Morphology, Proliferation, and p53-induce

11、d Apoptosis of HeLa Cervical Carcinoma Cells in Vitro. AsianPac J Cancer Prev. 2015;16(8):3195-201.2. Dou X, et al. Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect ofbetaine in a mouse model of alcoholic liver disease. Br J Pharmacol. 2014 Sep;171(17):4073-86.McePdfHeightCaution: Product has no