Methyl-β-cyclodextrin-Methyl-beta-cyclodextrin-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEMethyl-cyclodextrinCat. No.: HY-101461CAS No.: 128446-36-6Synonyms: Methyl-beta-cyclodextrin作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mLH2O : 50 mg/mL* me

2、ans soluble, but saturation unknown.請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn) 請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前?qǐng)先配制澄清的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.75 mg/mL (Infinity mM)

3、; Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.75 mg/mL (Infinity mM); Clear solution3. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.75 mg/mL (Infinity mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Methyl-cyclodextrin，種環(huán)狀七糖，可有效的消除細(xì)胞中的膽 醇; 抑制PEL細(xì)胞長(zhǎng)的IC50值為3.33-4.23 mM。IC50

4、 & Target IC50: 3.33-4.23 mM (PEL cell growth) 11/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE體外研究 Cyclodextrins are a family of cyclic oligosaccharides with a hydrophilic outer surface and a lipophilic centralcavity. Cyclodextrins molecules are relatively large with a number of hydrogen donors a

5、nd acceptors and,thus in general, they do not permeate lipophilic membranes. In the pharmaceutical industry, cyclodextrinshave mainly been used as complexing agents to increase aqueous solubility of poorly soluble drugs and toincrease their bioavailability and stability. Cyclodextrins are used in ph

6、armaceutical applications fornumerous purposes, including improving the bioavailability of drugs 2. Methyl-cyclodextrin quickly inducescaspase-dependent apoptosis in PEL cells via cholesterol depletion from the plasma membrane. Methyl-cyclodextrin inhibits the growth of all PEL cell lines in a dose-

7、dependent manner. The IC50 is 3.33-4.23 mMin each cell line 1. Methyl-b-cyclodextrin is a highly water soluble cyclic heptasaccharide consisting of a -glucopyranose unit, has been reported as the most effective agent for the depletion of cholesterol from cellsamong the various cholesterol-depleting

8、agents 1.體內(nèi)研究 In a PEL xenograft mouse model, methyl-b-cyclodextrin significantly inhibits the growth and invasion of PELcells without apparent adverse effects. Methyl-b-cyclodextrin-treated mice appears to be healthy, whereasnon-treated mice has a distended abdominal region. The body weights of con

9、trol are significantly higher thanthose of M-b-CyD treated mice. Methyl-b-cyclodextrin-treated mice has a significantly lower volume of ascitesthan that of non-treated mice 1. Studies in both humans and animals have shown that cyclodextrins can beused to improve drug delivery from almost any type of

10、 drug formulation. Currently, there are approximately30 different pharmaceutical products worldwide containing drug/cyclodextrins complexes in the market 2.PROTOCOLCell Assay 1 PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrationsof methyl-

11、cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37C. Subsequently, MTT (0.5mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 L of a 0.04 N HCl isadded to dissolve the crystals. Absorption values at 570 nm are determined 1.MCE has not independent

12、ly confirmed the accuracy of these methods. They are for reference only.Animal Mice: Female NRJ mice are intraperitoneally inoculated with BCBL-1 cells suspended in PBS. The mice areAdministration 1 then treated with intraperitoneal injections of PBS or methyl-cyclodextrin (500 mg/kg per day). Tumor

13、burdens are evaluated by measuring body weights and ascites 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) J Biomed Nanotechnol. 2018 Dec 1;14(12):2114-2123. ACS Chem Biol. 2018 Nov 16;13(11):3078-3086. Toxicol Appl Pharmacol. 2019 Aug.Se

14、e more customer validations on HYPERLINK / www.MedChemEREFERENCES2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE1. Gotoh K, et al. The antitumor effects of methyl-cyclodextrin against primary effusion lymphoma via the depletion of cholesterol fromlipid rafts. Biochem Biophys Res Commun. 2014 Dec 12;455(3-4):285-9.2. Tiwari G, et al. Cyclodextrins in delivery systems: Applications. J Pharm Bioallied Sci. 2010