Arg-Gly-Asp-Cys - Peptide - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEArg-Gly-Asp-CysCat. No.: HY-P0314CAS No.: 109292-46-8分式: CHNOS分量: 449.48Sequence: Arg-Gly-Asp-CysSequence Shortening: RGDC作靶點: Others作通路: Others儲存式: Powder -20C 3 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 50 mg/mL

2、 (111.24 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.2248 mL 11.1240 mL 22.2479 mL5 mM 0.4450 mL 2.2248 mL 4.4496 mL10 mM 0.2225 mL 1.1124 mL 2.2248 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Arg-Gly-Asp-Cys纖維粘連蛋與細(xì)胞粘附分的結(jié)合

3、序，能夠抑制板凝集以及纖維蛋原結(jié)合。體外研究RGDC immobilizes peptide onto DAH-CMTMC is found to be about 15.3 g/mg of chitosan derivative byamino acid analysis (AAA). RGDC-functionalized chitosan may lead to enhanced wound healing (viability1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemE140%). RGDC-functionalizes chito

4、san derivatives exhibit in vitro wound healing properties by enhancingfibroblast proliferation and adhesion. RGDC-DAH-CMTMC favors cell growth and an increase in cellularproliferation compared to the control cells 1.PROTOCOLCell Assay 1 Human precursor dermal fibroblasts (HDF, human dermal progenito

5、r cells, 12 week male donor) are use inthe assay. WST-1 assay is used to assess the viability of HDF when incubated with chitosan derivatives. Forthis study, HDF are seeded in a 96-well plate at a density of 6103 cells/cm2. To each well, 100 L of cellsuspension is added and incubated for 48 h in ord

6、er to allow cell attachment. DMEM is then replaced by 100L of CMTMC and RGDC-DAH-CMTMC suspension at concentrations of 0.25 mg/mL, 0.5 mg/mL and 1mg/mL, respectively. Cell viability under polymer incubation is evaluated during 2, 4 and 7 days. SDS (1%) isused as negative control. The polymer solutio

7、n is changed every 3 days. 100 L of WST-1 (1:10 dilution inDMEM) are added in each well after removing the polymer suspension and incubated for 0.5-2 h.Absorbance is recorded with a BioTek Microplate reader at two different wavelengths (450 and 690 nm). Theviability is presented as percentage compar

8、ed to the positive control group (cells in DMEM supplementedwith 10% fetal calf serum). All experiments are carried out in triplicates.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Patrulea V, et al. Peptide-decorated chitosan derivatives enhance fibroblast adhesion and proliferation in wound healing. CarbohydrPolym. 2016 May 20;142:114-23.McePdfHeightCaution: Product has not been fully validated for medical applica