Ionomycin-SQ23377-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEIonomycinCat. No.: HY-13434CAS No.: 56092-81-0Synonyms: SQ23377分式: CHO分量: 709.01作靶點(diǎn): Calcium Channel作通路: Membrane Transporter/Ion Channel; Neuronal Signaling儲(chǔ)存式: Powder -20C 3 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) E

2、thanol : 10 mg/mL (14.10 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 1.4104 mL 7.0521 mL 14.1042 mL5 mM 0.2821 mL 1.4104 mL 2.8208 mL10 mM 0.1410 mL 0.7052 mL 1.4104 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Ionomycin (SQ23

3、377)個(gè)鈣離載體 (Calcium ionophore)，也來(lái)于鏈霉屬 (Streptomycesconglobatus) 的種抗素。IC50 & Target Calcium channel 1.體外研究Ionomycin (SQ23377) is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEATCC 31005 1. It is observed that LCLC 103H cells

4、overexpressing the catalytic subunit of -calpain rapidlyunderwent cell death after treatment with ionomycin. The first signs of Ionomycin (SQ23377)-induced celldeath are detected 3 h after addition of the ionophore. Addition of 2 M Ionomycin (SQ23377) to LCLC 103Hcells causes an instantaneous increa

5、se in intracellular Ca2+ concentration from 50 to 180 nM. Remarkably,calcium concentrations are raised transiently to 0.8-1.5 M. DNA and protein analysis in Ionomycin(SQ23377)-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typicalof caspase-mediated apoptosis. Th

6、ree hours after addition of ionomycin, the percentage of early apoptoticcells approximately doubles. Necrosis could be detected in 1-5% of the Ionomycin (SQ23377) treated cellsas supported by simultaneously positive fluorescein labeling and propidium iodide uptake. Caspaseactivation in whole cells w

7、as followed by monitoring the increase in activity against Ac-DEVD-amc followingIonomycin (SQ23377) treatment 2. Aging (Albany NY). 2019 Aug 19;11.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Liu C, et al. Characterization of ionomycin as a calcium ionophore. J Biol Chem. 1978 Sep 10;253(17):5892-4.2. Gil-Parrado S, et al. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J Biol Chem. 2002 Jul26;277(30):27217-26.McePdfHeightCaution: Product has not been fully validated for medical applications.For r