G-418-disulfate-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEG-418disulfateCat.No.:HY-17561CASNo.:108321-42-2分?式:C??H??N?O??S?分?量:692.71作?靶點:Bacterial;Antibiotic作?通路:Anti-infection儲存?式:4°C,storedundernitrogen,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(storedundernitrogen,awayfrommoisture)溶解性數據體外實驗H2O:125mg/mL(180.45mM;Needultrasonic)掃描?維碼，運?溶解?案計算器獲得適合您實驗體系的溶解?案MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.4436mL7.2180mL14.4361mL5mM0.2887mL1.4436mL2.8872mL10mM0.1444mL0.7218mL1.4436mL請根據產品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存?式和期限。體內實驗請根據您的實驗動物和給藥?式選擇適當的溶解?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：為保證實驗結果的可靠性，澄的儲備液可以根據儲存條件，適當保存；體內實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶1.請依序添加每種溶劑：PBSSolubility:50mg/mL(72.18mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY1/3www.MedChemEwww.MedChemE?物活性G-418disulfate(Geneticinsulfate)?種氨糖苷類抗?素，抑制真核?物和原核?物的蛋?質合成。G-418disulfate?種常?的真核細胞選擇性藥劑。體外研究G418sulfate,anaminoglycosideneomycinanalogue,interfereswithproteinsynthesisandhasbeenusedextensivelyforselectionofmammaliancelllinesthatpossessneomycinresistance(NR).Theneomycinresistance(neo)geneisfrequentlyusedineukaryoticvectorsasadominantselectablegene.G418canbecovalentlyboundtoasialoorosomucoid(AsOR)toformaconjugateforhepatocyte-specifictargetingandtoxicity[2].ThehumanGD3synthasecDNAwastransfectedintoMDA-MB231cells,andG-418bulkselectionwasusedtoselectcellsstablyoverexpressingtheGD3synthase[2].Anaminoglycosideantibiotic,G418,hasbeenshowntobeaninhibitorofmanypro-andeukaryotesatconcentrationsfrom1-300microgram/ml[4].PROTOCOLAnimalTocharacterizethesensitivityofthetrypanosomepopulationstoG418invivo,bloodstreamformsofT.Administration[3]bruceibruceiGUTat3.1andT.bruceibruceiGUTat3.1/BBR3areexpandedseparatelyinsublethallyirradiatedmice.Priortothefirstpeakofparasitemia,trypanosomesarecollected,andaliquotscontaining106trypanosomesareinoculatedintraperitoneallyintomice.Twenty-fourhoursfollowinginfection,themicearedividedintogroupsandtreatedwithG418atadoseof10,20,30,40,50,or80mg/kgofbodyweight(bw)byinoculatingintraperitoneally0.2mLofthedruginsterilewater.At24and48hfollowingthefirsttreatment,G418isadministeredtoanimalsineachgroupatthesamedoseasbefore,resultinginthreetreatmentspermouse.RepeateddrugtreatmentsarenecessarytoensurecompleteeliminationofnontransfectedGUTat3.1parasitesfromthemice.Micearethenmonitoreddaily,for33days,forthepresenceofparasitesbymicroscopicexaminationofwet-bloodfilms.Animalsfoundtobeparasitemicarerecordedandthenremovedfromtheexperiment.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產品發(fā)表的科研?獻?CellDeathDiffer.2020Jun;27(6):1981-1997.?CellRep.2021Mar30;34(13):108906.?CellRep.2020Oct20;33(3):108287.?CellDeathDis.2020Jun4;11(6):420.?CellDeathDis.2019May28;10(6):413.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].Giordano-SantiniR,etal.Anantibioticselectionmarkerfornematodetransgenesis.NatMethods.2010;7(9):721-723.[2].VolarevicM,etal.AnovelG418conjugateresultsintargetedselectionofgeneticallyprotectedhepatocyteswithoutbystandertoxicity.BioconjugChem.2007;18(6):1965-1971.[3].KwonKM,etal.DisialylGD2gangliosidesuppressesICAM-1-mediatedinvasivenessinhumanbreastcancerMDA-MB231cells.IntJ2/3www.MedChemEwww.MedChemEBiolSci.2017;13(3):265-275.Published2017Feb12.[4].DaviesJ,etal.Anewselectiveagentforeukaryoticcloningvectors.AmJTropMedHyg.1980;29(5Suppl):1089-1092.McePdfHeight關注MCE中國公眾號，

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