分子生物學(xué)(雙語(yǔ))2012 A

安徽大學(xué)2012—2013學(xué)年第1學(xué)期《分子生物學(xué)（雙語(yǔ)》考試試卷（A卷）--- （閉卷 時(shí)間120分鐘）------ 考場(chǎng)登記表序號(hào)----號(hào)-- 題號(hào) 一號(hào)---學(xué) - 得分---- 閱卷人--

二 三 四 五 六 七 總分一線(xiàn)----------名線(xiàn)--姓 --訂-----裝----超-訂勿--業(yè)-業(yè)題-專(zhuān)-專(zhuān)--答--------級(jí)-級(jí)-年-年----裝-------系-系--/ -院 ----

詞解釋題（每小題3分，共36分） 得分semiconservativereplicationBaseExcisionRepairrhodependentterminatorpolycistronicmRNACvalueparadoxISelementprimaseelongationfactortopoisomeraseRNAinterferenceenhancer第1頁(yè) 共4頁(yè)得分二、填空題（每空1.5分，共21分）得分DuringDNAunwindsdouble-strandedDNA,and removesRNAprimerstheOkazakifragmentswithitsto3'exonucleaseactivity.2.2.Atypicalprokaryoticpromoterhastwoconsensussequences,whicharetermed and .Theidealspacingbetweenthetwoconsensussequencesis .Transpositionoccursviatwotypesofmechanisms.Oneiscalled ;theothercalled .Innegativecontrol,theregulatorproteinactsasa toblocktranscriptionoftheoperon.5.InSOSrepairsystem,thetwokeyregulatoryproteinsare5.InSOSrepairsystem,thetwokeyregulatoryproteinsare(positive/negative)regulator.andLexA.LexAisa6. ,whichlocatesupstreamofthestartcodonofthemRNA,binds3'endofthe16SrRNAintheribosometoinitiateproteinsynthesis7.IneukaryotesthetRNAgenesaretranscribedbyRNApolymerase.8. Whenadouble-strandDNAisdenatured,theabsorbanceatA260will(decrease/increase).9. Thegenerationofantibodydiversityismainlydue.得分三、單項(xiàng)選擇題（每小題1.5分，共21分.請(qǐng)?zhí)钊胂卤硐鄳?yīng)題號(hào)欄中）得分11234567891011121314DNApolymerasecanaddnucleotidestoafree3′endofagrowingDNAchain B. afree5′endofagrowingDNAchainC. eithera3′or5′endofachain D. a5′endofRNAprimerE. alloftheaboveWhatisthefirstproteintobindtoaeukaryoticpromoter,andatwhichsitedoesitbind?TFIIDbindstoaTATAbox. B. RNApolymeraseIIbindstoaTATAbox.Aregulatorproteinbindstoaregulatorregion.Anactivatorproteinbindstoanenhancer.Splicingistheprocessthatdoeswhichofthefollowing?AddmultipleadenosinebasestotheendofaprimaryRNAtranscriptRemoveexonsandjoinintronsRemoveintronsandjoinexonsRemovemutatedregionsofprimarytranscriptRNA WhichsubunitoftheE.coiNApolymeraseholoenzymecatalyzesthesynthesisofRNA?α-subunit B. β-subunit第2頁(yè) 共4頁(yè)得分C. rhofactor D. sigmafactor得分TheDNAintranscriptionallyactivechromatintendstobehighlymethylated B. fully-methylatedC. Under-methylatedornon--methylated D. Semi-methylatedThemostefficientcontrolofeukaryoticgeneexpressionisachievedatthelevelofReplication. B. translationinitiationC.post-transcription D. post-translation.E..transcriptioninitiation.Translationisterminatedwhenastopcodonispresentedatthe .A.EsiteB. AsiteC.PsiteD. eitherAorBE. eitherAorCInaraoperon,Cproteincanserveasanactivator B.arepressorC.eitherarepressororanactivatordependingonpresence/absenceofarabinoseD.botharepressorandanactivatorirrespectiveofpresence/absenceofarabinoseIn astandardrecombinantDNAprocedure,thestepwhichfollowstheconstructionofrecombinantDNAmoleculeshouldbeA re-ligationofthevectorsligatingthevectorwiththegeneofinterestdigestingthevectorwitharestrictionendonucleasetransformationofthemoleculeintohostcellsWhichofthefollowingisNOTastepinaPCRreaction:A.AnnealingB.ExtensionC.DenaturationD.dehydrationOneimportantdifferencebetweenDNAreplicationinprokaryotesandeukaryotesisthatprokaryotesdonotuseenzymesinthereplicationprocessthereisonlyonereplicationorigininprokaryotestherearenoOkazakifragmentsinprokaryotesreplicationisconservative,notsemiconservative,inprokaryotesAframeshiftmutationcouldresultfromabaseinsertiononly. B.abasedeletiononly.C.abasesubstitutiononly. D. eitheraninsertionoradeletionofabase.E.deletionofthreeconsecutivebases.WhichoftheenzymesbelowisinvolvedinrepairofboththyminedimeranddeaminatecytosineinE.coliDNApolymeraseI B. PhotolyaseC.UracilN-glycosylase D. APendonucleaseAnIRE(ironresponseelement)regulatestheexpressionofironmetabolismrelatedgenesbybybindingtoIRE-BPbyformingahair-pinstructureatpost-transcriptionalortranslationallevelall