AgBr-NP@CTMAB抗白色念珠菌活性及在PMMA相關(guān)應(yīng)用研究

AgBr-NP@CTMAB抗白色念珠菌活性及在PMMA相關(guān)應(yīng)用研究AgBr-NP@CTMAB抗白色念珠菌活性及在PMMA相關(guān)應(yīng)用研究

摘要：

隨著醫(yī)療科技的進(jìn)步和人們生活水平的提高，人類對(duì)高品質(zhì)牙齒修復(fù)材料的需求也越來越大。然而，缺陷材料可能會(huì)導(dǎo)致口腔疾病的發(fā)生和發(fā)展。白色念珠菌是一種常見的口腔真菌病原，對(duì)PMMA修復(fù)材料具有可怕的生物附著性和異味生成能力。因此，開發(fā)一種安全高效的AgBr納米顆粒@CTMAB修復(fù)材料已成為目前研究的熱點(diǎn)。本文利用溶液法制備了不同質(zhì)量比AgBr-NP@CTMAB納米顆粒，并研究了它們對(duì)不同白色念珠菌菌株生長(zhǎng)的抑制作用。結(jié)果表明，AgBr-NP@CTMAB抗白色念珠菌活性與CTMAB濃度密切相關(guān)，并與AgBr納米顆粒的質(zhì)量比有關(guān)。此外，在搭配使用AgBr-NP@CTMAB與PMMA修復(fù)材料后，材料的生物附著性和異味生成能力均不斷降低，其力學(xué)性能略有下降，但仍能夠滿足需要。因此，AgBr-NP@CTMAB修復(fù)材料具有很好的應(yīng)用前景。

關(guān)鍵詞：白色念珠菌；AgBr-NP@CTMAB；PMMA修復(fù)材料；生物附著性；異味生成；力學(xué)性能

AgBr-NP@CTMABAntifungalActivityagainstCandidaalbicansandItsApplicationinPMMAMaterials

Abstract:

Withtheprogressofmedicaltechnologyandtheimprovementofpeople'slivingstandards,thedemandforhigh-qualitydentalrestorativematerialsisincreasing.However,defectivematerialsmayleadtotheoccurrenceanddevelopmentoforaldiseases.Candidaalbicansisacommonoralfungalpathogenthathasterriblebio-adhesiveandodor-generatingcapabilitiesonPMMArestorativematerials.Therefore,developingasafeandefficientAgBrnanocrystals@CTMABrestorativematerialhasbecomethecurrentresearchhotspot.Inthispaper,AgBr-NP@CTMABnanocrystalswithdifferentmassratioswerepreparedbysolutionmethod,andtheirinhibitoryeffectsonthegrowthofdifferentCandidaalbicansstrainswerestudied.TheresultsshowedthattheantifungalactivityofAgBr-NP@CTMABwascloselyrelatedtotheconcentrationofCTMABandthemassratioofAgBrnanocrystals.Inaddition,afterusingAgBr-NP@CTMABincombinationwithPMMArestorativematerials,thebio-adhesiveandodor-generatingcapabilitiesofthematerialscontinuedtodecrease,andtheirmechanicalpropertiesslightlydecreasedbutstillmettherequirements.Therefore,AgBr-NP@CTMABrestorativematerialhasagoodapplicationprospect.

Keywords:Candidaalbicans;AgBr-NP@CTMAB;PMMArestorativematerial;bio-adhesive;odorgeneration;mechanicalpropertiesThebio-adhesivepropertiesofdentalrestorativematerialscanleadtotheformationofbiofilmsonthesurface,whichcancauseadverseeffectsonoralhealth.Candidaalbicansisoneofthemostcommonmicroorganismsfoundindentalbiofilmsandisassociatedwithoraldiseasessuchascandidiasis.Toaddressthisissue,AgBr-NP@CTMABhasbeenproposedasapotentialsolutiontoreducebio-adhesivepropertiesandodorgenerationofPMMArestorativematerials.

TheadditionofAgBr-NP@CTMABtoPMMArestorativematerialssignificantlyreducedthebio-adhesivepropertiesofthematerials.ThemechanismbehindthisphenomenonisthatthepositivelychargedCTMABmoleculesonthesurfaceofAgBr-NP@CTMABcaninteractwiththenegativelychargedcellwallsofmicroorganisms,includingCandidaalbicans,topreventtheiradhesiontothematerialsurface.TheAgBrnanocrystalsinAgBr-NP@CTMABalsohaveantimicrobialpropertiesthatcaninhibitthegrowthofmicroorganisms.

Inadditiontoreducingbio-adhesiveproperties,theuseofAgBr-NP@CTMABalsoreducedtheodorgenerationofPMMArestorativematerials.ThisisbecausetheantimicrobialpropertiesofAgBr-NP@CTMABcaninhibitthegrowthofodor-causingmicroorganisms.Thecombinationofthetwomechanismsleadstoareductionintheformationandaccumulationofbiofilmsonthesurfaceofthematerial,whichcanimproveoralhealth.

AlthoughtheadditionofAgBr-NP@CTMABslightlydecreasedthemechanicalpropertiesofPMMArestorativematerials,thematerialsstillmettherequirementsfordentalapplications.Therefore,AgBr-NP@CTMABrestorativematerialhasagoodapplicationprospectindentalpractice.Futureresearchcanfurtherexplorethelong-termantimicrobialefficacyandbiocompatibilityofAgBr-NP@CTMABrestorativematerialsInadditiontothepotentialbenefitsoutlinedabove,therearesomepotentialdrawbacksandlimitationsassociatedwiththeuseofAgBr-NP@CTMABrestorativematerialsindentalpractice.Oneimportantconsiderationisthepossibilityoftoxicityorotheradverseeffectsassociatedwiththeuseofsilvernanoparticles.Whilesilverhaswell-knownantimicrobialproperties,thereareconcernsaboutthepotentialforsilvertoaccumulateinthebodyovertimeandcauseharm.Somestudieshavesuggestedthatnanoparticlesmaybemoretoxicthanlargerparticlesofthesamematerial,duetotheirsmallsizeandincreasedsurfacearea.Therefore,itwillbeimportanttocarefullyevaluatethesafetyandtoxicityofAgBr-NP@CTMABrestorativematerialsbeforewidespreaduseindentalpractice.

AnotherpotentiallimitationofAgBr-NP@CTMABrestorativematerialsisthattheirantimicrobialpropertiesmaynotbeeffectiveagainstalltypesofbacteria.Whilesilverhasbeenshowntobeeffectiveagainstabroadrangeofmicroorganisms,somespeciesmaybemoreresistanttoitseffects.Inaddition,thereisthepossibilityofdevelopingbacterialresistancetosilverovertime,whichwouldlimititslong-termefficacy.

Finally,itisworthnotingthatAgBr-NP@CTMABrestorativematerialsarenotastandalonesolutionforimprovingoralhealth.Whiletheymayhelptopreventtheformationofbiofilmsondentalrestorations,theydonotaddressotherimportantfactorsthatcontributetooraldisease,suchaspoororalhygieneoradiethighinsugarandothercarbohydrates.Therefore,itwillbeimportanttocontinuetopromotegoodoralhealthpracticesalongsidetheuseofthesematerials.

Overall,thedevelopmentofAgBr-NP@CTMABrestorativematerialsrepresentsapromisingadvanceinthefieldofdentalmaterialsscience.ByincorporatingsilvernanoparticlesintoPMMArestorativematerials,itmaybepossibletoimprovetheirantimicrobialpropertiesandreducetheriskofinfectionandothercomplications.However,furtherresearchisneededtofullyevaluatethesafetyandefficacyofthesematerials,andtorefinetheirformulationandapplicationforoptimalclinicaluseInadditiontothepotentialbenefitsofAgBr-NP@CTMABrestorativematerials,therearealsosomechallengesandlimitationsthatneedtobeaddressed.Oneissueisthepossiblecytotoxicityandgenotoxicityofsilvernanoparticles,whichcanhavenegativeeffectsonhumancellsandtissues.AlthoughthereisevidencethattheAgBr-NP@CTMABmaterialshavelowtoxicityandbiocompatibility,morestudiesareneededtoconfirmtheirsafetyandlong-termeffectsonoralhealthandoverallhealth.

Anotherchallengeisthecomplexityandvariabilityofdentalrestorativeprocedures,whichcanaffectthepropertiesandperformanceofthematerialsused.Factorssuchasmoisture,temperature,pH,andmechanicalstresscaninfluencethebonding,durability,andaestheticsofdentalrestorations,andmayalsointeractwiththesilvernanoparticlesintheAgBr-NP@CTMABmaterials.Therefore,itisimportanttooptimizetheprocessingandhandlingofthesematerialstoensureconsistentandreliableoutcomes.

Furthermore,theclinicalapplicationsandindicationsofAgBr-NP@CTMABmaterialsmaydependonthespecificneedsandconditionsofdifferentpatientsanddentalcases.Whiletheantimicrobialpropertiesofsilvernanoparticlesaregenerallybeneficialforpreventingandtreatingdentalcariesandotherinfections,theymaynotbenecessaryorappropriateforalltypesofrestorationsorpatients.Insomecases,alternativematerialssuchascompositeresinsorceramicrestorationsmaybemoresuitableoraesthetic.

Overall,thedevelopmentandevaluationofAgBr-NP@CTMABrestorativematerialsisapromisingareaofresearchforimprovingtheperformanceandsafetyofdentalrestorations.Withfu