生物分離工程雙語(yǔ)版-2

1

IntroductionConsidering

protein

properties

for

bioseparation(1)

size(2)

charge(3)

biological

affinity(4)

hydrophobicity-solubilityProducts

from

mammalian

cell

techniques(1)

enzyme(2)

hormones(3)

nonantibody

immunoregulators(4)

monoclonal

antibodies(5)

tumor

specific

antigens第二頁(yè)，共七十二頁(yè)。1

IntroductionInitial

bioseparation

importance(1)

filtration(2)

centrifugation(3)

chromatographyClassified

heuristics（論據(jù)，規(guī)則）(1)

unit

operation

methods(2)

design

step

sequence(3)

species

(components

properties)(4)

composition第四頁(yè)，共七十二頁(yè)。第五頁(yè)，共七十二頁(yè)。第六頁(yè)，共七十二頁(yè)。1

IntroductionThree

factors

of

designing

bioseparation

processes(1)

purity(2)

cost(3)

marketMaterial

state

and

choice

of

separation

methodsMaterial

secreted

(

ultrafiltration,

centrifugation)Not

secreted

material

(cell

disruption,

solid-liquidseparation)Material

in

liquid

(

ultrafiltration,

adsorption)Material

in

solid

(extraction

into

aqueous

solution)第七頁(yè)，共七十二頁(yè)。New

and

wordsProtacte

lawsuits訴訟Deleterious有害的，有毒的Vexing煩惱Hierarchic分等級(jí)Subdomain亞域Domains蛋白質(zhì)結(jié)構(gòu)的域Subunit亞單位Endoplasmic

reticulum內(nèi)質(zhì)網(wǎng)Disulfide

bond二硫鍵Academia學(xué)術(shù)界Delicate脆弱的Flowchart流程圖Cytoplasmic細(xì)胞質(zhì)的Periplasmic外周胞質(zhì)Osmotic

透urea脲Guanidine胍第八頁(yè)，共七十二頁(yè)。II

Product

excretion

from

the

cell

or

celldisruptionExcreted

protein

advantage(1)

contamination

level

low(2)

easy

downstream

processing

steps(3)

not

require

cell

disruption(4)

avoid

handling

viscous

fluids

and

proteinase release(5)

facility

extraction

of

products(6)

signal

peptide

determines

secretion第九頁(yè)，共七十二頁(yè)。Cell

disruption(1)energy-intensive(強(qiáng)烈）

and

violent(2)

without

destroying

interest

protein(3)contain(兼容）

process(bacteria,yeast,

mammalian,

plant,

and

insectsources)(4)

be

controlled(5)

be

validated第十頁(yè)，共七十二頁(yè)。Products

requiring

cell

disruptionVaccines(tetanus(破傷風(fēng)）,meningitis（腦膜炎)Glucokinase（葡萄糖激酶）Glycerokinase

（甘油激酶）Invertase

（轉(zhuǎn)化酶）Toxin

(enterotoxin)Subcellular

components

(mitochondrai,

chloroplast)Intracellular

constituents

(DNA,

RNA

preparation,

andvirus-like

particles)Recombinant

insulinRecombinant

growth

hormoneProtein

A

and

G.第十一頁(yè)，共七十二頁(yè)。Ideal

large

scale

cell

disrupter(1)

disrupt

tough

organisms(2)

mechanism

of

cell

disruption

should

be

wellunderstood(3)

sterilizable(4)containable（兼容）

and

validatable(5)

amenable

to

being

made

automatic(6)continuous

and

compact（緊湊的）(7)

economical第十二頁(yè)，共七十二頁(yè)。Cell

disruption

goalObjective

function(monetary（金錢(qián)）

value

ofproduct)Small

scale

cell

disruption(1)

ultrasonic(2)freeze

thawing(熔化）(3)

force

cells

through

very

small

orifices

at

hig pressure(4)

glass

or

porcelain

ball

mills第十三頁(yè)，共七十二頁(yè)。Large

scale

cell

disruptionHomogenizerStructure

(positive

displacement

pump,

pistons,

nozzle)Mechanisms(1)

Shear(2)Cavitation

（氣穴）(3)impingement(沖擊）Condition(1)

concentration

(4-175

g

dry

wt/liter)(2)

pre

sure

(30-95

MPa)(3)

passes

number

(~5time)(4)

modeled

by

equation(5)

wild-type

E.

coli

ce

ls

more

di

ficult

to

disrupt

(heatinduction

at

42C)第十四頁(yè)，共七十二頁(yè)。Ln(1/(1-R))=KNPΓ第十五頁(yè)，共七十二頁(yè)。第十六頁(yè)，共七十二頁(yè)。Delineating

mechanisms

of

cell

disruption(1)

facilitate

controlling(2)

validating(3)

reproducible(4)

better

design

and

optimize

equipment(5)Turbulent

eddies

(湍流渦旋）Responsible

for

cell

disruption

oscillate)(6)

shear

stress,

turbulence

byimpingement第十七頁(yè)，共七十二頁(yè)。Enzymatic

lysis

cell

disruption(1)

a

lytic

protease

(dissolve

the

outer

part

of

the

cell

wall(2)

endo-β-(l-3)glucanase（葡聚糖酶）

to

disintergrate

glucan

net(3)

source（酶的來(lái)源）

and

culture

system

kinetic

analysisA

understanding

the

systemB

designing

the

small

and

large

scale

systemsC

cytophaga

（吞噬細(xì)胞）system

exhibits

high

proteaseactivity,

higher

conversion

and

higher

lysisrate

at

longer

timeD

oerskovia

systems

high

glucanase

low

protease

activity,contain

some

inhibitorsE

an

initial

lag

in

yeast

lysis

by

both

enzymeF

simple

two

step

model

(

cell

mass

solubilized,

proteinhydrolyzed

to

peptides)G

Mishaelis-Menten

kinetic

equation

predict

cell

wall

lysisH

production

of

food

grade

single

cell

protein第十八頁(yè)，共七十二頁(yè)。New

words

and

vocabularyAmylase淀粉酶Modulate調(diào)節(jié)Interferons干擾素Viscous

fluids粘性流體Signal

peptide信號(hào)肽Carboxypeptidase羧肽酶Alkaline

phosphatase堿性磷酸酯酶Glyceraldehyde-3-phosphate

dehydrogenase甘油醛-3-磷酸脫氫酶Reduced

nicotinamide

adenine還原型尼克酰胺腺嘌呤Dinucleotide二核苷Reductase還原酶Hydrophobic疏水的Hydrophilic親水的第十九頁(yè)，共七十二頁(yè)。New

words

and

vocabularies

(2)Pathogens病源體Ball

mills球磨機(jī)Resonance共鳴，回聲Vibration振動(dòng)，擺動(dòng)Dissipate分散Pinpointing最佳點(diǎn),針尖Oscillate振蕩Candida

utilis假絲酵母Extrusion噴出Sapphire蘭寶石Jewel寶石Thermocouple熱電偶Sleeve袖子，套Hydrodynamic流體力學(xué)Hydroxylase

enzyme羥化酶Synergistic增效，協(xié)同作用Glucan葡聚糖Morphology第二十頁(yè)，共七十二頁(yè)。III

initial

fractionationPrimary

isolation

step(1)

adsorption(2)

extraction(3)

precipitation(4)

distillation(5)

filtration(6)

solvent

evaporationRequirementSignificant

volume

reduction第二十一頁(yè)，共七十二頁(yè)。Interest

product

in

solution(1)

gradually

remove

impurities(2)

increase

product

concentration(3)

chose

methods

depends

on

scale

and

organism(4)

washing

stages

followed

by

dewatering

stepYeasts

easy

to

deal

with(1)

10

μm

in

diameter(2)

easily

flocculated(3)

centrifugation(4)

filtration(5)

cross-flow

filtration第二十二頁(yè)，共七十二頁(yè)。Analysis

disrupted

baker’s

yeast

suspensions(1)

viscosity(2)

density(3)

sedimentationMeaning(1)

minimize

degradation

cell

wall

following

rupture(2)

minimize

cell

fragment

proportion(3)

restrict

viscosity

increase(4)

correlation

between

lab

and

pilot

scale

centrifugesor

industry(5)

estimate

separation

costs

at

an

early

stage第二十三頁(yè)，共七十二頁(yè)。First

crude

separation

or

final

productdewater

steps(1)

adsorption(2)

precipitation(3)

membrane

separationA

cross

flow

filtrationB

microfiltrationC

ultrafiltration(4)

liquid-liquid

extraction第二十四頁(yè)，共七十二頁(yè)。Aqueous

two-phase

partition(1)

polymer

+

salt

,

polymer

+

polymer(2)

easy

to

scale

up(3)

biocompatible(4)

rapid

(with

centrifugation)(5)

purification

and

concentration

of

biological product(6)

cell

particles

and

nucleic

acids

removed(7)

large

volume

reduction第二十五頁(yè)，共七十二頁(yè)。A

adsorption(1)

purify

protein(2)

difficult

to

removing

particulate

using packed

or

fixed

beds(3)

particulate

entrapped

in

voids

of

the

beds(4)

excessive

pressure

drops

and

deformation of

adsorbent(5)

expanded

bed(6)

fluidized

bed第二十六頁(yè)，共七十二頁(yè)。Expanded

bed

adsorption(1)

eliminate

a

step

for

removal

of

cell

or

debris(2)

scale

up

simple

(similar

to

packed

bed)(3)

combine

clarification,

concentration

and purification(4)

could

be

used

for

viscose

fluid(5)

high

biomass

content

of

feedstock(6)

expanded

bed

stabilized

by

wide

distribution of

particle

size第二十七頁(yè)，共七十二頁(yè)。第二十八頁(yè)，共七十二頁(yè)。第二十九頁(yè)，共七十二頁(yè)。用吸附劑分離的抗生素第三十頁(yè)，共七十二頁(yè)。New

words

and

vocabularyColloidal

（膠狀的）Glycan

（聚糖）Lysate

（溶解物）Aerosol（懸浮微粒,氣溶膠）Particulate

（微粒）Fluidized

bed

（沸騰床）Streptomycin

（鏈霉素）Novobiocin

（新生霉素）Annexin

V

（膜聯(lián)蛋白[一類(lèi)被鈣離子活化后可與膜磷脂結(jié)合的蛋白）Scout（偵察）Washing

（洗滌）Elution

（洗脫）第三十一頁(yè)，共七十二頁(yè)。New

words

and

vocabularyReverse

phase

high

pressure

liquid

phasechromatography(反相HPLC）Circumvented

（包圍）Hybridoma

（雜合細(xì)胞，(淋巴細(xì)胞)雜交瘤）Penicillin

（青霉素）Tetracycline

（四環(huán)素）Cephalosporin

C

（頭孢菌素C）Divinyl

benzene

（二乙烯苯）Styrene

（苯乙烯）第三十二頁(yè)，共七十二頁(yè)。B

membrane

separationFunction

:

Clarification

and

sterilizingProblems(1)

Solid-liquid

interface

adsorption(2)

significant

surface

area

(500cm2)Membrane

materials(1)

hydroxyl-modified

hydrophilic

polyamide(2)hydrophilic

polyvinylidene

fluoride(PVDF)聚偏二乙烯氟化物）(3)

nylon-66Interest

protein(1)

insulin

(100%

recovery)(2)

bovine

serum

albumin

(BSA)

98%

recovery(3)

immunoglobulin第三十三頁(yè)，共七十二頁(yè)。Minimizing

protein

loss(1)

low-binging

protein

membrane(2)

dilute

protein

solution(3)

expensive(4)

examine

prefilters

and

final

filters第三十四頁(yè)，共七十二頁(yè)。Remove

of

virus(1)

thermal

inactivation(2)

chemical

treatment(3)

denaturant

(urea)(4)β-propiolactone（丙內(nèi)酯）-UV

light

exposure(5)

membrane

filtrationA

base

on

sizeB

reproducibleC

predictableD

completeE

validatableF

high

protein

recovery第三十五頁(yè)，共七十二頁(yè)。New

words

and

vocabularyInsulinBovine

serum

albumin

（牛血清蛋白）Immunoglobulin

（免疫球蛋白）Scale-down

（按比例縮減）Discernible（可辯別的）Steady

state（穩(wěn)態(tài)）Asymptotically

（漸近的）Low-binding

protein

membraneβ-propiolactone

UV

light

（β-丙內(nèi)酯-紫外光）Monotonically（無(wú)變化的，單調(diào)的）Platelet

（血小板）ImmunodiseaseHydraulic

permeability

（水

透性）Filtration

resistance（過(guò)濾阻力）Pure

water

permeability(PWP)純水透過(guò)性第三十六頁(yè)，共七十二頁(yè)。VocabulariesDomaindisulfide

bonds,signal

peptide,turbulent

eddiesAqueous

two-phase

partitioningexpanded

bed

adsorption

fluidizedbedpacked

bedreverse-phase

high

pressure

liquid-phase

chromatographyfloculatebiocompatiblescaled-downsteady-statelangmuir

isotherm第三十七頁(yè)，共七十二頁(yè)。Questions1

Which

advantages

secreted

proteins?2

Which

differences

between

Mammalian

cell

proteinsand

microorganism

proteins

?3

Which

methods

disrupting

cell

in

laboratory4

What

is

the

most

important

in

early

part

ofbioseparation

?5

What

advantage

have

expanded

bed

adsorption

bycomparing

with

packed

bed

?6

How

to

remove

virus

or

virus-like

in

protein

solution

?第三十八頁(yè)，共七十二頁(yè)。Chapter

2Sec第三十九頁(yè)，共七十二頁(yè)。C

UltrafiltrationExample：

antibodies

production(1)

antibodies

applicationA

diagnosisB

affinity

ligand

(cytokines

and

blood-clotting

factors)(2)

membrane

material

(cellulose

triacetate

)(3)

useA

50-100-fold

concentrationB

minimizing

shear

forcesC

tangential

and

frame

ultrafiltration

systemD

gel

polarization

（濃差極化）

reducedF

acceptable

flux

rate第四十頁(yè)，共七十二頁(yè)。Denaturation

during

ultrafiltratio(1)

conformational

change(2)

instability(3)

immunogenicity(免疫原性）Influencing

ultrafiltration

factorsA

electrostatic

interaction

(attraction,

repulsiB

Hydrophobicity第四十一頁(yè)，共七十二頁(yè)。New

words

andvocabularyMandate

（命令，要求）Diagnostics診斷學(xué)Cytokines細(xì)胞因子Blood

clotting

factors

（凝血因子）Tangential

plate切向板，錯(cuò)流過(guò)濾板

Undermine（破壞）Integrity

（完整）Tryptic

β-casein

peptides

β-

蛋白肽Antihypertensive

（抗高血壓的）Immunomodulatory免疫調(diào)節(jié)劑Plasmin血纖維蛋白溶解酶Transmission輸送Detrimental有害的Interfacial

tension表面張力Emulsion

liquid

membrane乳狀液膜Carrier載體

Diffusivity擴(kuò)散率第四十二頁(yè)，共七十二頁(yè)。D

cross-flow

filtrationAffinity

cross-flow

filtration(1)

combining

bioadsorption

and

membrane

in

a single

step(2)

escort

supportsA

chemically

and

mechanically

stableB

possess

a

large

specific

areaC

Negligible

nonspecific

adsorptionD

easily

modifiable

to

incorporate

ligands(3)

high

protein

loss

in

washing

step第四十三頁(yè)，共七十二頁(yè)。第四十四頁(yè)，共七十二頁(yè)。New

words

and

vocabulariesAffinity

cross-flow

filtrationEscort簇Supernatant

上清液Monomolecular

layerInnermost內(nèi)心的，最里面的Langmiur

isothermGlutaradehydeSchiff

basesSodium

borohydrideMicroparticles第四十五頁(yè)，共七十二頁(yè)。E

Emulsion

liquid

membranePenicillin

G

extraction

by

emulsion

liquid

membraneApplicationConcentrate

weak

acids,

bases,

metal

ion

and

biomaterialPenicillin

extraction(1)

Carrier

:

amine(2)

pH

adjuster

Na2CO3

at

pH

5-8

,

penicillin

Gstable(3)

9

times

concentration第四十六頁(yè)，共七十二頁(yè)。第四十七頁(yè)，共七十二頁(yè)。F

ExtractionInsulin

purification

process(1)

extraction

by

ethanol(2)

acidification

at

pH

2(3)

neutralization

with

calcium

carbonate(4)

vacuum

concentration(5)

salt

precipitation(6)

reprecipitation

by

isoelectric

point(7)

gel

filtration

chromatography

or

ion

exchange chromatographyDifficult

information

available(1)

effect

of

different

process

variables(2)

sequencing

of

different

subprocesses(3)

scale

level

of

operation第四十八頁(yè)，共七十二頁(yè)。New

words

and

vocabulariesEmulsion

liquid

membrane乳化液膜Amine胺Carrier載體Pancreas胰腺Hyperglycemia高血糖Diabetes

mellitus糖尿病Porcine

origin豬器官I(mǎi)soelectric

point等電點(diǎn)Reverse-phase

chromatography反相層析Scarce缺乏的，不足的Proprietary所有權(quán)第四十九頁(yè)，共七十二頁(yè)。G

precipitationApplying

high

resolution

technique

at

earlier

stage(1)

reduce

volume(2)

reduce

chemical

consumptionAffinity

precipitation

using

chitosan

as

a

ligandPrincipleSoluble

ligand

binding

to

target

protein

lead

to

precipitate(1)

aggregate(2)

heterobifunctional

ligandsIsolating

trypsin

with

STI

chitosan第五十頁(yè)，共七十二頁(yè)。第五十一頁(yè)，共七十二頁(yè)。Precipitation

factors(1)

salt

and

cosolvents

(supersaturation,

seedparticles

,

crystallization,

agglomerationα-lactalbumin

separationWhey

components

:

lactose,

proteins

,

salts

and

residual

fat(1)

1:1

binding

calcium

ion

(α-1A)(2)

conformational

change

(unfolding

transitions,surface

hydrophobicity)(3)

selectively

separating

α–

lactalbumin((α-1A)and

β-

lactoglubulin

at

pH

isoelectric

point

and

above

50

℃(4)

nonspecific

precipitation第五十二頁(yè)，共七十二頁(yè)。Affinity

precipitation

using

ligand

modifiedphospholipid

as

a

ligand

carrierPrincipleSoluble

ligand

binding

target

protein

to

form

precipitateHeterobifunctional

ligand

(binding

target

molecule

and

facilitate

precipitof

liand-target

protein)Biotin

ligand

binding

avidin(1)

rapid

binding(2)

slow

hydrophobic

interaction

between

nonpolar

alkyl

sidechains

of

phospholipids(3)

high

yield

and

purity

of

avidinDrawbacks(1)

difficult

to

obtain

stable

and

inexpensive

bis-ligands,polyligands

and

ligand

carriers(2)

adsorption

capacity

low(3)

nonspecific

adsorption

degree

high第五十三頁(yè)，共七十二頁(yè)。第五十四頁(yè)，共七十二頁(yè)。Affinity

precipitation

mechanismTwo

mechanism(1)

primary

effectProtein

cross-linking

and

forming

networkwith

ligand(2)

second

effectSolubility

decrease

of

ligand

carrier

due

to

pH

,

temperature,

and

salt

concentration第五十五頁(yè)，共七十二頁(yè)。STI

immobilized

on

PLS

as

an

affinityagent

for

trypsin

precipitation(1)

materialA

artificial

solution

containing

BSA

and

trypsinB

a

crude

pancreatic

extract(2)

salt

precipitated

PLS

due

to

osmotic

shock(3)

PLS

redispersed

in

solution

after

removing

salt

by

centrifuge(4)

PLS

had

large

specific

surface

area

and

ligand

coupling

capacity(5)

lack

of

nonspecific

adsorption(6)

91%

trypsin

coprecipitated

in

artificial

solution

and

87%

trypsin

recovered·in

precipitate

by

0.01

M

NaOH(7)

STI-PLS

repeated

three

times(8)

Purification

factor

was

about

six(9)

loss

of

binding

capacity

(93.3%,

87.7%,

83.3%)(10)

80%

activity

recovery

and

specific

activity

with

1X104

U/mg(11)

precipitate

and

redisperse

10

times

by

added

salt第五十六頁(yè)，共七十二頁(yè)。New

words

and

vocabulariesAffinity

precipitation親和沉淀Heterobifunctional

ligand異種雙功能試劑Chitosan殼聚糖Oligomeric寡聚體的Soybean

trypsin

inhibitor大豆蛋白酶抑制劑Chymotrypsin胰凝乳蛋白酶，糜蛋白酶Supersaturation過(guò)飽和α-lactalbumin乳白蛋白Whey

protein乳清蛋白Mammary

gland乳腺Trytophan脯氨酸Transitions過(guò)渡Turbidity混濁Surfactant表面活性劑Phospholipids磷脂Avidin生物素結(jié)合蛋白Biotin生物素Dimyristoylphosphatidylethanolamine二肉豆蔻酰磷酸乙醇胺Octaethylene

glycol

mono-n-dodecyl-ether八乙烯醇單十二烷基醚Hastened促進(jìn)，加速Polymerized

liposome聚合脂質(zhì)體Redispers再分散Steric

hindrance空間位阻第五十七頁(yè)，共七十二頁(yè)。H

chromatographic

proceduresLarge

scale

purification(1)

yield(2)

purity(3)

operation

scale(4)

chromatography

(limited

extent

in

initial

step)Example

2.11Staphylococcal

enterotoxin

B

purification

using

chromatography(1)

Two

ion

exchange

steps(2)

One

gel

filtration

step(3)

high

linear

flow

rates

at

low

pressure<

5

kP(4)

culture

supernatantA

(centrifugation,

sterile

filtration,

adsorption).B

content

0.4mg/LC

protein

content

11mg/mlD

purity

0.004%E

volume

400

L第五十八頁(yè)，共七十二頁(yè)。Pharmacia

column

370

KS

purification(1)

protein

content

from

11

to1.5mg/ml(2)

SEB

content

from

0.00042

to

0.12

mg/ml(3)

purification

factor

2000Biological

molecules

product

standards(1)

DNA

<10

pg/dose(2)

no

viruses(3)

pyrogen

<

300

endotoxins

IU/dose第五十九頁(yè)，共七十二頁(yè)。Example

2.12Divinylbenzene-polystyrene

resin

separation(1)

analysis

of

thermodynamics

using

HPLC(2)

linear

adsorption

isotherm

above

293-313K(3)

temperature-dependent

adsorption

isotherm constants

ΔS,ΔH(4)

pore

size

diameter(5)

polarity

of

resin(6)

chromatography

is

only

a

choice

for industrial

separation第六十頁(yè)，共七十二頁(yè)。New

words

and

vocabulariesStaphylococcal

enterotoxin葡萄球菌外毒素Methicillin甲氧苯青霉素Pharmacia

columnDose劑量Pyrogen熱原Miniscule小字的Divinylbenzene

polystyrene

copolymer苯乙烯-二乙烯苯共聚物Aspartame門(mén)冬氨酰苯丙氨酸甲酯（比蔗糖甜200倍的甜味劑）Phenylalanine苯丙氨酸Aspartic

acid天門(mén)冬氨酸Asparagine天門(mén)冬素，天冬酰胺酸Adsorption

isotherm吸附等溫線Homologues類(lèi)似物，同族體第六十一頁(yè)，共七十二頁(yè)。IV

high-resolution

fractionationPrevious

purification

steps(1)

volume

reduction(2)

clarification(3)

adjustment

of

crude

extractChromatography

steps(1)

cation

exchange(2)

anion

exchange(3)

affinity

chromatography(4)

hydrophobic

chromatography第六十二頁(yè)，共七十二頁(yè)。A

chromatography

proceduresAffinity

chromatography(1)

nonspecific

adsorption(2)

expensive

labile

ligand(3)

difficulties

of

adsorbent

sterilization(4)

biomimetic

ligand(5)

natural

ligand

expensive

and

decreased

activity

whenimmobilized.(6)

pseudo-ligand(7)

ligand

leakage

and

product

contaminationLarge

scale

purification(1)

remove

low

levels

of

impurities(2)

reduce

pyrogen(3)

exchange

into

buffer

systems

suitable

for

formulation(4)

gel

filtration

chromatography

has

low

productivity第六十三頁(yè)，共七十二頁(yè)?！EB:Staphylococcal

enterotoxin葡萄球菌外毒素第六十四頁(yè)，共七十二頁(yè)。Final

products

analysis(1)

SDS-PAGE

(single

band)(2)

gel

filtration

(single

peak)Developing

large-scale

purification

step(1)

new

media(2)

equipment(3)

optimization第六十五頁(yè)，共七十二頁(yè)。Ultrafast

HPLC(1)

instrumentationA

selectivityB

sensitivity(2)

resinA

rapid

association

and

dissociationB

material

geometry

play

significant

role(3)

obtain

quantitative

product

recovery

estimation(4)

visualize

wide

variety

of

host

cell

impuritiesPosttranslational

modification

(

deamidation,

proteolysisand

glycosation)(5)

rapid

HPLC

significanceA

monitoring

processB

optimization

of

protein

purityC

developing

large

scale

process第六十六頁(yè)，共七十二頁(yè)。Purification

and

characterization

of

lampregastric

lipase(1)

play

an

important

role

in

fat

digestion

in

newborn

animals(2)

enhance

cheese

food

flavors(3)

initial

purification

step