血涂片制備染色及白細胞形態(tài)觀察【-】課件

血涂片制備染色及白細胞形態(tài)觀察臨床檢驗基礎與輸血教研室血涂片制備染色及白細胞形態(tài)觀察臨床檢驗基礎與輸血教研室1根據不同的細胞種類及細胞的不同成分，對酸性及堿性染料的結合能力不同，而使各種細胞呈現出各自的特點。掌握血涂片的制備和染色方法及白細胞的正常形態(tài)原理

目的根據不同的細胞種類及細胞的不同成分，對酸性及堿性染料的結合能2器材載玻片、推片、洗耳球、顯微鏡、微量吸管、香柏油、乙醇-乙醚溶液、拭鏡紙試劑瑞氏染液、磷酸緩沖液標本抗凝血器材載玻片、推片、洗耳球、顯微鏡、微量吸管、香柏油、乙醇3血涂片制備推片載玻片血液將推片勻速向前，勿停頓。涂片的厚薄取決于速度和角度：快、大(厚)。慢、?。ū。┮粡垵M意的血涂片應厚薄均勻頭體尾鮮明涂片干燥后用鉛筆在血涂片的頭部寫上姓名和編號李四B123血涂片制備推片載玻片血液將推片勻速向前，勿停頓。涂片的4血涂片染色勿沖洗再加瑞氏染色Ⅱ液5-8滴后將Ⅰ液和Ⅱ液充分混勻靜置10分鐘直接流水沖洗3-5分鐘（切勿先倒掉染液）涂片經水洗干燥后用油鏡分類計數100個白細胞李四123涂片干燥后加瑞氏染色Ⅰ液5-8滴覆蓋整個血膜1分鐘血涂片染色勿沖洗再加瑞氏染色Ⅱ液5-8滴后將Ⅰ液和Ⅱ液5血涂片的觀察低倍鏡觀察1.染色情況(滿意、偏酸、偏堿）2.細胞分布情況油鏡下分類計數：體尾交界處。油鏡分類時應按一定走向，不要重復計數。李四123血涂片的觀察低倍鏡觀察李四1236中性桿狀核粒細胞中性桿狀核粒細胞7

中性分葉核粒細胞中性分葉核粒細胞8

嗜酸性粒細胞嗜酸性粒細胞9嗜堿性粒細胞嗜堿性粒細胞10

單核細胞單核細胞111.本次實驗的關鍵環(huán)節(jié)是什么？

2.每人分類的結果與本組其他人使用同一標本的同學是否一致，分析原因。分析討論分析討論12K+oN3sR6vVazYdC$hG-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8xXcB#fF&jI)mM1qP4tT8AZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vU9zYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8A#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uT9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbA$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL1qP4tT8xWbA#fE%iH)mL0pP4tS7wWbAZeD%iH(lK0pO3sS7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-oN2rQ5vU9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9y#gF&jI-nM1qP5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ+oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB%iI)mL0pP4tS7wWbAZeD%iH(lL0pO3sS7wVazZeD$hG(lK+oO3sR6vVazYdC$hG*kK+oN2rR6vU9yYdC!gF*kJ-nN2rQ5uU9yXcB!gF&jJ-nM1qQ5uT8xXcB#fE&jI)mM1qP4tT8xWbA#fE%iH)mL0pP4tS7wWbAZeD%iH(lL0pO3sS7wVazZeD$hG(lK+oO3sR6vVazYdC$hG*kK+oN2rR6vU9yYdC!gF*kJ-nN2uT8yXcB#fF&jI)nM1qP4uT8xWbB#fE%jI)mL0qP4tS7xWbAZeE%iH(mL0pO3tS7wVaAZeD$iH(lK+pO3sR6wVazYdD$hG*lK+oN2sR6vU9zYdC!gG*kJ-oN2rQ5vU9yXcC!gF&kJ-nM1rQ5uT8yXcB#fF&jI)nM1qP4uT8xWbB#fE%jI)mL0qP4tS7xWbAZeE%iH(mL0pO7wVazYeD$hG(lK+oN3sR6vUazYdC$hG*kJ+oN2rQ6vU9yXdC!gF*kJ-nM2rQ5uT9yXcB!gF&jI-nM1qP5uT8xWcB#fE&jI)mL1qP4tS8xWbAZfE%iH)mL0pO4tS7wVbAZeD%iH(lK0pO3sR7wVazYeD$hG(lK+oN3sR6vUazYdC$hG*kJ+oN2rQ6vU9yXgF&jJ-nM1qQ5uT8x