網(wǎng)織紅細(xì)胞檢測課件

網(wǎng)織練細(xì)胞檢測技術(shù)及臨床應(yīng)用任燕歌網(wǎng)織練細(xì)胞檢測技術(shù)1概念●網(wǎng)織紅細(xì)胞reticulocyte,Ret或R)界于晚幼紅細(xì)胞和成熟紅細(xì)胞之間的過渡階段細(xì)胞。因其胞質(zhì)中殘存的核糖核酸經(jīng)堿性染料活體染色后,呈現(xiàn)點(diǎn)狀或線網(wǎng)狀結(jié)構(gòu),故名網(wǎng)織紅細(xì)胞。概念2多能單能千細(xì)干細(xì)胞成熟紅細(xì)原始紅細(xì)紅細(xì)胞發(fā)育過程圖網(wǎng)織早幼紅細(xì)紅細(xì)晚幼紅細(xì)紅細(xì)胞多能3●oe●。A網(wǎng)織紅細(xì)胞:絲球形、網(wǎng)型、破網(wǎng)型、點(diǎn)粒型●oe●。4網(wǎng)織紅細(xì)胞檢測技術(shù)網(wǎng)織紅細(xì)胞檢測技術(shù)5網(wǎng)織紅細(xì)胞計(jì)數(shù)方法活體染色手工鏡檢●血液自動分析儀法●流式細(xì)胞術(shù)檢測法網(wǎng)織紅細(xì)胞計(jì)數(shù)方法6網(wǎng)織紅細(xì)胞檢測課件7操作方法①取干凈試管加入10g/L煌焦油藍(lán)溶液2~3滴,然后加入等量的血液混勻后放置15~20min,使紅細(xì)胞充分染色;②取少許染色好的血液,在潔凈載玻片上推成薄而均勻的血膜,千燥后用瑞氏染液復(fù)染(也可不復(fù)染)③在顯微鏡低倍鏡下選擇細(xì)胞分布均勻著色淸晰的部位,用油鏡在血片上計(jì)數(shù)至少1000個(gè)紅細(xì)胞分布區(qū)域內(nèi)的網(wǎng)織紅細(xì)胞再除以1000計(jì)算出網(wǎng)織紅細(xì)胞的百分率。同時(shí)進(jìn)行紅細(xì)胞計(jì)數(shù),可計(jì)算網(wǎng)織紅細(xì)胞的絕對值操作方法8網(wǎng)織紅細(xì)胞檢測課件9活體染色圖oOee活體染色圖10網(wǎng)織紅細(xì)胞檢測課件11網(wǎng)織紅細(xì)胞檢測課件12網(wǎng)織紅細(xì)胞檢測課件13網(wǎng)織紅細(xì)胞檢測課件14網(wǎng)織紅細(xì)胞檢測課件15網(wǎng)織紅細(xì)胞檢測課件16網(wǎng)織紅細(xì)胞檢測課件17網(wǎng)織紅細(xì)胞檢測課件18網(wǎng)織紅細(xì)胞檢測課件19網(wǎng)織紅細(xì)胞檢測課件20網(wǎng)織紅細(xì)胞檢測課件21網(wǎng)織紅細(xì)胞檢測課件22網(wǎng)織紅細(xì)胞檢測課件23網(wǎng)織紅細(xì)胞檢測課件24網(wǎng)織紅細(xì)胞檢測課件25網(wǎng)織紅細(xì)胞檢測課件26網(wǎng)織紅細(xì)胞檢測課件27網(wǎng)織紅細(xì)胞檢測課件28網(wǎng)織紅細(xì)胞檢測課件29網(wǎng)織紅細(xì)胞檢測課件