NoncodingRNA非編碼RNA.ppt

1、Page 1,Repertor:Xiaoning Yuan Oct.9.2012,Noncoding RNA,Irnne.MP,Cheng G,Wieland.S,et al，2007,Noncoding RNA,Introduction Classification Research method Mechanism The relationship with major disease,1.Introduction,Noncoding RNA(ncRNA) is a kind of transcripts without the ability of coding proteins,whi

2、ch exist widely in highly eukaryotic.The amount of the ncRNAtranscripts is up to 98 of the whole genomes transcripts. The difference in molecular structure and expressive pattern from other kinds of RNA endows them specific biological functions. Although exact functions of most ncRNA are not well kn

3、own.it has been demonstrated that ncRNA was associated with some human diseases,2.Classification,1830nt: miRNA，siRNA，piRNA 30200nt: rRNA，tRNA，snRNA， snoRNA，SLRNA，SRPRNA More than 200nt:mRNA-like noncoding RNA and so on,3.Researching method,Method for identification: bioinformation establish specific

4、 cDNA libraries DNA Tiling chip,Identification,3.Researching method,Method for reserching function: Clustering methed Nock out,4. Mechanism,a. Noncoding RNA can interact with DNA, for example, noncoding RNA can modify chromosome configuration, and then affect transcription activity of local region o

5、f DNA.,4.Mechanism,b. Noncoding RNA can interact with mRNA to adjust the edition, stability and the translation of mRNA.,4.Mechanism,c. Noncoding RNA ,as a regulater for synergismcan,can bind to protein factor and change the biological activity of the protein. chemotatic noncoding RNA also recruit p

6、rotein to specific site of a cell.,4.Mechanism,d.Noncoding RNA which overlap itself and be a complex higher-order structure,privide space or binding site for molecular and protein factor. besides,somebody hold the opinion that RNA molecular simimulate prion protein conversion.,5.The relationship with major disease,a.Neurological disease: schizophrenosis autism melancholia hyperactivity,The relationship with major disease,b.cancer The H19 noncoding RNA is essential for human tumor growth The signaling pathway mediated by ncRNA associate with hunman tumor growth,