[生物醫(yī)藥論文精品a]茉莉酸甲酯對丹參莖葉丹酚酸和丹酚酸b含量的影響

1茉莉酸甲酯對丹參莖葉丹酚酸和丹酚酸B含量的影響李明馮世陽廣東藥學(xué)院中藥學(xué)院廣州510006摘要目的探討茉莉酸甲酯METHYJASMONAT,MJ對栽培丹參的藥效成分影響，并為丹參地上部分的開發(fā)利用提供一定的研究依據(jù)。方法采用比色法和HPLC法，以丹酚酸和丹酚酸B為檢測指標，對茉莉酸甲酯處理的丹參莖葉樣品進行檢測。結(jié)果丹參種苗經(jīng)茉莉酸甲酯噴施處理后的48H內(nèi)，其莖葉中丹酚酸和丹酚酸B含量均高于對照，在所測試范圍510A1A2A3510A1A4的濃度中，隨濃度的升高而含量增加。結(jié)論外施茉莉酸甲酯能促進丹參莖葉中的丹酚酸和丹酚酸B含量的積累，可以考慮在生產(chǎn)中加以應(yīng)用，并注意今后在丹參的種植及采收中地上部分的利用。關(guān)G19202G16801G726丹參G727茉莉酸甲酯G727丹酚酸G727丹酚酸BSTUDYONTHEEFFECTOFTOTALPHENOLICACIDSANDSALVIANOLICACIDBCONTENTINTHELEAVESANDSTEMSOFSALVIAMILTIORRHIZATREATEDBYMETHYJASMONATLIMINGFENGSHIYANGG54G70HG82G82G79G82G73CHG76G81G72G86G72G48G68G87G72G85G76G68G48G72G71G76G70G68G15G42G88G68G81G74G71G82G81G74PG68G85G80G68G70G72G88G87G76G70G68G79G56G81G76G89G72G85G86G76G87G92G15G42G88G68G81G74G93HG82G8851000G25G15CHG76G81G68OBJECTIVEOBJECTIVEG55G82G87HG72G72G73G73G72G70G87G82G73G80G72G87HG92G79G77G68G86G80G82G81G68G87G72G708G48G45G709G82G81G80G72G71G76G70G76G81G72G70G82G80G83G82G86G76G87G76G82G81G82G73G70G88G79G87G76G89G68G85SALVIAMILTIORRHIZA，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丹參G708SALVIAMILTIORRHIZABGEG709為G2779G5430G12197植G10301丹參的G5190G10169G7693及G7693莖，其G7389效成分G2265G6336G8712G9354G5627和G14038G9354G5627成分。G14038G9354G5627成分G1039G16213G7171G1120G14832G18272G12879G2282G2524G10301G708G2265G6336丹參G18242G451G19556丹參G18242G12573G709，G8712G9354G5627成分G1039G16213為酚酸G12879G2282G2524G10301G708G2265G6336丹參G13044G451G2419G1811G14602G18287G451G17867G17857A0A5A6A7A8A9A10A11A12A13A14A15A16A17A0A5A18A19A20A21A19A22A23A24A25A26A27A28A18A24A29A30A20A31A26A32A33A32A34A35A32A36A37A38A39A40A41A42A43A44A11A45A46A47A48A49A50A51A52A53A54A15A55A56A57A39A58A59A60A61A62A63A8A63A60A22A30A64A23A23A64A65A66A61A67A68A68A69A70A68A60A69A70A71A72A73A74A63A75A24A20A21A20A29A22A19A20A22A23A202G20333酸G451G13055G14621酸G451G2666G2869酸G451丹酚酸SALVIANOLICACIDG36G451BG12573G709。對于丹參的G8712G9354G5627酚酸G12879G2282G2524G10301，G5353G17227G1114G2319藥學(xué)G4490的G7509G3835G18337G16282，G1075G11013于丹參的G1268G13491應(yīng)用G7053G5347為G8712G10018G9094，G17829G1135G5192G7481，對于其G8712G9354G5627酚酸G12879G2282G2524G10301的研究G17751G3822A76A77A78A79A80。丹酚酸BG7171G11458G2081研究G17751G3822的丹酚酸G1055一，在G8847G11115G5527G15892G12661G11154G11161G451G5942G5627G13937G9826G451G6251G13937G13432G13512G2282G451G6251G2172G14045G12921樣G11840G2282和G6925G2904G16772G5530G2163能障礙G12573G7053面發(fā)揮著G18337G16213作用A76A81A80。最新研究表明，丹參葉中的酚酸G12879含量與G7693中相似，G7389G1135甚至高于G7693部A82A83A78A84A85。茉莉酸甲酯G708MJG709G7171植G10301響應(yīng)G11161G2419G10301G451害蟲G12573G3822種環(huán)境脅迫的共同信號分子，廣泛存在于高G12573植G10301體內(nèi)，并作為新型的植G10301激G13044，在調(diào)節(jié)植G10301生長發(fā)育G451光G2524作用G451G6251逆反應(yīng)G12573G7053面G17227著G18337G16213的作用，外施到植G10301體可以達到一定的逆境脅迫和調(diào)節(jié)效應(yīng)A76A86A78A87A80。G7389報道外源G5627MJG12879G2282G2524G10301能G7389效刺激植G10301次生代謝G10301的生G10301G2524成，可G5353G17227G3822種植G10301次生產(chǎn)G10301的迅速積累A76A88A80。G11458G2081G7389利用G48G45研究誘導(dǎo)丹參毛狀G7693中的G7389效成分的積累的報道A76A88A80，本研究旨在利用MJ作為誘導(dǎo)子G15在丹參的生產(chǎn)中進行田間噴施實驗，探索MJ對丹酚酸B的調(diào)控機制G15以及MJ在丹參生產(chǎn)中的應(yīng)用及丹參地上部分的進一步G2524理開發(fā)提供研究依據(jù)。1材料與G7053法1G171實驗材料和實驗地點供試材料為丹參G708SALVIAMILTIORRHIZABG74G72G709種苗G15購于山東菏澤丹參種植基地，茉莉酸甲酯G708MJG709為美國G54G76G74G80G68公司產(chǎn)品。實驗地位于廣東藥學(xué)院G3835學(xué)城校區(qū)藥用植G10301實驗園。1G172實驗設(shè)計及處理實驗設(shè)5個處理G726對照G708丹參噴清G8712G709G727噴施MEJA510A89A90G80G82G79/LG727噴施MEJA510A89A87G80G82G79/LG727噴施MEJA510A89A84G80G82G79/LG727噴施MEJA510A89A81G80G82G79/L。小區(qū)面積1G80A79，4次G18337復(fù)，隨機區(qū)組實驗設(shè)計。MEJA和清G8712均在出齊三片復(fù)葉時噴施，將配好的各種濃度的MJG9354G9094用噴壺均勻地噴到對應(yīng)各組葉片上，以噴滿全部葉片為度，噴后立即套上保鮮袋。噴施后48小時取樣，取每銖苗的全部小葉片，將葉置G250烘G5190箱烘G25H，取出粉碎，過50G11458篩后用棕色廣口瓶保存。1G17G22丹參G5647酚酸含量測定1G17G22G171G2419G1811G14602G18287標G1946G7366G13459制定G2419G1811G14602G18287標G1946G9094G726G12946G4506G12228取G2419G1811G14602G182878G1750G80G74，加G8712G9354G16311并定G4493至20G80G79G7084253G173G74/G80G79，作為對照品G9354G9094。分G2047G12946G4506G2572取G2419G1811G14602G18287對照品G90940G174G4510G178G4511G172G4511G17G25G4512G170G80G79加G8712至2G80L，分G2047加0G175G8G49G68G49G50A795G170G80LG6930置5G80G76G81，加G184910G8G36G79G49G50A81A810G175G80G79，G6930置5G80G76G81，加G18491G80G82G79/LG49G68G50HG90945G170G80G79，G6930置10G80G76G81后，以G7186色G2070為G12366G11345，在500G81G80處測G5483G2572光G1552。以濃度G59為G8190G3364標，G2572光G1552G60為G13449G3364標，G7053G12255為G92G320G170014G91G140G170004，G85G320G17G28G28G281，G13459G5627范圍為2G25G161G220G173G74/G80L。1G17G22G172G5647酚酸的測定G12946G4506G12228取樣品粉G74230G172G74，置50G80LG4493量瓶中，加2G8G11428酸G8712G9354G9094G1343412G80L，G9036G888515H，加酸G8712G15929G17287量，G6403G668310G80G76G81，經(jīng)G5190G10169G9400G13452過G9400，G5335G2447G2033G9400G9094，G12946G4506量取G13505G9400G90945G80L，置分G9094G9443G7019中，加G49G68CG790G175G74，G1069酸G1069酯G14807取G22次，每次5G80L，G8712G9032G14988G5190，G8543G11053G10301以G7092G8712G1069G18267G9354G16311并定量G17728G12239到10G80LG4493量瓶中，G1889用G7092G8712G1069G18267G12244G18334至G2063度，G6683勻，經(jīng)G5190G10169G9400G13452過G9400，G5335G2447G2033G9400G9094，G12946G4506量取G9400G90941G80LG6365G2419G1811G14602G18287標G1946G7366G13459測定的G7053法測定G2572光度，計G12651生藥G8712G9354G5627G5647酚酸的含量。1G174丹參葉片丹酚酸B含量測定1G174G171色G16901G7477G1226色G16901G7621G726G39G76G78G80G68G50G39G54C184G17G25250G80G805G173G80G15G8981G2172相G726甲G18267G16G1069G14108G160G1725G8G11979酸G708G220G72610G726G250G709，G8981速為1G80G79/G80G76G81，檢測G8886長G72628G25G81G80G727G7621G9213G220。1G174G172對照品G9354G9094的制G3803取丹酚酸B對照品G22G80G74，置10G80G79G4493量瓶中，加G265G8甲G18267G17878量，G9354G16311，G1889用G265G8甲G18267定G4493至G2063度，G6683勻，即G5483。1G174G17G22供試品G9354G9094的制G3803取丹參樣品粉G7423G134340G1725G74，G12946G4506G12228定，置G1867G3634G19193G5430瓶中，G12946G4506加G1849G265G8甲G18267G9354G909425G80L，G4506G3634，G12228定G18337量，加G9921G3250G89811H，G6930G1931，G1889G12228定G18337量，用G265G8甲G18267G9354G9094G15929G1817G1014G3845的G9354G2070量，G6683勻，過G9400，取G9400G9094，即G5483。1G175G174G13459G5627關(guān)G13007考G4531G12946G4506G12228取丹酚酸B對照品G9354G9094，分G2047G12946G4506G2572取2G22037G79，4G22037G79，8G22037G79，10G22037G79，12G22037G79G45114G22037G79分G2047進樣，測定G4804面積，以G4804面積G60對進樣量G59進行G13459G5627G3250G5414，G5483G3250G5414G7053G12255G60G322G22G2502G59G16G22522G174，G85G320G17G28G28G28G28G727表明丹酚酸B進樣量在0G17G2504G173G74G16G22G17G2524G173G74范圍內(nèi)與G4804面積成G14403好的G13459G5627關(guān)G13007。1G175G175G12946G4506度試驗取樣品G9354G909420G79，G18337復(fù)進樣G25次。G13479G7536丹酚酸BG4804面積G53G54G39G321G1725G8，G18337復(fù)G56274G14403好。1G175G17G25G12295定G5627試驗取樣品G9354G909410L，分G2047在制G3803后0G4512G4514G4518G45112G45124H測定，G13479G7536丹酚酸BG4804面積G53G54G39G321G1758G8，表明24H內(nèi)G9354G9094G12295定。1G175G17G26G3250收G10587試驗G5191行在G25G1233樣品G9354G9094中加G1849丹酚酸B標G1946品G9354G90941G17G26G80G79，依法G6817作，測定，G13479G7536G5191均G3250收G10587為G288G170G8，G53G54G39G321G170G8。丹酚酸B的HPLCG3282G16277G32821。2G17G13479G7536與分G75242G171G993同濃度MJ對丹參地上部分G8712G9354G5627G5647酚酸含量的影響實驗G13479G7536G7186G12046G708G16277表1G709，在所測試的范圍內(nèi)，丹參地上部分莖G451葉的G8712G9354G5627G5647酚酸含量隨茉莉酸甲酯濃度的升高而增加，葉中的G8712G9354G5627G5647酚酸含量增加的G12255度G17751莖中增加的G3835，G3926茉莉酸甲酯濃度為510A89A84G48時，葉的G5647酚酸含量G17751對照增加G1114G252G705，莖中G5647酚酸含量G17751對照增加G1114G22G25G705G727茉莉酸甲酯濃度在510A89A81G7477G1226G991，葉的G5647酚酸含量G17751對照增加G1114100G705，莖中G5647酚酸含量G17751對照增加G111448G705。A91A92A93A94A95A96A97A98A99A100A101A102A103A104A105A106A107A108A109A99A110A111A112A113A114A115A116A117A118A119A92A120A121A122A122A119A123A124A125A122A126A127A125A128A129A116A118A130A131A116A128A125A118A131A123A116A123A131A132A123A125A128A124A119A128A124A131A128A124A133A119A129A124A119A134A129A116A128A132A118A119A116A130A119A129A125A122A135A136A137A138A139A136A140A139A137A141A139A142A143A143A144A139A145A136A146A147A148A149A150A151A152A153A154A155A156A157A158A159A160A161A162A163A164A165A166A167A168A169A170A168A171A170A168A172A173A174A175A176A177A170A159A160A161A162A163A164A165A166A167A168A178A170A168A171A170A168A172A173A174A175A176A177A170A179A180A181A182A183A184A185A180A182A180A186A181A182A187A181A185A180A182A180A183A188A183A189A186A180A190A191A192A182A192A181A185A180A182A180A192A181A182A193A186A185A180A182A180A186A194A183A189A186A180A195A196A192A182A184A197A185A180A182A180A192A192A182A186A187A185A180A182A180A192A198A183A189A186A180A195A199A184A182A180A193A185A180A182A180A181A192A182A200A193A185A180A182A180A181A201A183A189A186A180A195A202A183A182A181A184A185A180A182A180A181A192A182A193A197A185A180A182A180A1812G172G993同濃度MJ對丹參地上部分丹酚酸B含量的影響實驗G13479G7536G708G32822G16G25，表2G709G7186G12046，在測試的范圍內(nèi)，丹參地上部分莖G451葉的丹酚酸B含量隨MJ濃度的升高而增加，其中葉的丹酚酸B含量增加的G12255度G17751莖的G3835，G3926茉莉酸甲酯濃度為510A190A203G48時，葉的丹酚酸B含量G17751對照增加G11148G22G705，莖中丹酚酸B含量G17751對照增加G11142G28G705G727MJ濃度在510A190A204G48G7477G1226G991，葉的丹酚酸B含量G17751對照增加G111411G26G705，莖中丹酚酸B含量G17751對照增加G11145G26G705。5A205A206A207A208A209A210A211A212A213A214A215A216A217A218A205A219A220A221A222A207A206A215A216A217A218A218A223A224A225A226A227A228A225A222A224A227A226A225A229A230A227A231A232A221A227A233A225A231A221A234A227A234A221A235A211A221A233A236A237A238A239A240A241A239A242A243A243A244A239A245A246A205A247A207A248A214A179A215A216A217A218A205A219A220A221A222A207A247A215A216A217A218A218A223A224A225A226A227A228A225A222A224A227A226A225A229A236A237A238A239A240A241A239A242A243A243A244A239A245A246A230A227A226A249A231A250A230A179A205A251A207A248A214A188A215A216A217A218A205A219A220A221A222A207A251A215A216A217A218A218A223A224A225A226A227A228A225A222A224A227A226A225A229A236A237A238A239A240A241A239A242A243A243A244A239A245A246A230A227A226A249A231A250A230A188A205A252A207A248A214A194A215A216A217A218A205A219A220A221A222A207A252A215A216A217A218A218A223A224A225A226A227A228A225A222A224A227A226A225A229A236A237A238A239A240A241A239A242A243A243A244A239A245A246A230A227A226A249A231A250A230A1946A205A253A207A248A214A198A215A216A217A218A205A219A220A221A222A207A252A215A216A217A218A218A223A224A225A226A227A228A225A222A224A227A226A225A229A236A237A238A239A240A241A239A242A243A243A244A239A245A246A230A227A226A249A231A250A230A198A205A254A207A248A214A201A215A216A217A218A205A219A220A221A222A207A253A215A216A217A218A218A223A224A225A226A227A228A225A222A224A227A226A225A229A236A237A238A239A240A241A239A242A243A243A244A239A245A246A230A227A226A249A231A250A230A201A255A247A207A8A0A210A1A2A212A208A13A16A3A4A208A209A210A211A21A5A6A7A23A9A10A11A12A26A227A14A231A250A247A207A32A229A229A250A234A228A225A229A37A15A225A233SALVIANOLICACIDBA234A225A233A228A250A233A228A221A233A228A223A250A230A228A250A226A230A227A233A235A231A250A227A232A250A230A225A229A236A246A240A17A239A246A238A239A240A241A239A242A243A243A244A239A245A246A74A18A19A149A150A151A152A153A154A155A156A157A158A169A96A159A165A151A112A166A167A168A113A170A178A96A159A165A151A112A166A167A168A113A170A179A180A180A182A200A192A185A180A182A180A186A180A182A200A200A185A180A182A180A184A188A183A189A186A180A195A20A180A182A187A186A185A180A182A180A181A180A182A200A197A185A180A182A180A181A194A183A189A186A180A195A196A180A182A193A183A185A180A182A180A181A180A182A197A187A185A180A182A180A186A198A183A189A186A180A195A199A186A182A186A181A185A180A182A180A184A180A182A193A181A185A180A182A180A181A201A183A189A186A180A195A202A186A182A192A192A185A180A182A180A192A186A182A186A180A185A180A182A180A1812G17G22MJ在G993同時間內(nèi)對丹參地上部分G8712G9354G5627G5647酚酸含量的影響以510A190A203G48的MJ噴施丹參葉面，在G993同時間檢測葉和莖中的G5647酚酸含量，G13479G7536G16277G3282G26。實驗G13479G7536表明，隨處理時間的G5322長，丹參葉和莖中的G5647酚酸含量增加，其中葉中的G5647酚酸含量增加的G5145度G17751莖的G3835。在處理G262H和120H后，丹參葉中G5647酚酸含量分G2047G17751對照增加G1114G282G8和108G8，莖中G5647酚酸含量分G2047G17751對照增加G1114G220G8和44G8。7A22A24A25A27A28A29A30A22A25A28A28A31A33A25A24A25A22A34A35A36A38A39A40A41A42A43A44A45A46A47A48A114A49A50A51A168A65A170A205A69A52A53A70A54A55A56A23MJA212A208A13A16A3A4A57A209A210A21A5A23A6A7A220A221A222A207A69A32A229A229A250A234A228A225A229A37A15A221A233A235A221A229A229A250A224A250A233A228A228A224A250A227A228A250A235A228A221A226A250A230A225A233A230A227A231A232A221A227A233A225A231A221A234A227A234A221A235A221A233A228A223A250A230A228A250A226A230A227A233A235A231A250A227A232A250A230A225A229A236A246A240A17A239A246A238A239A240A241A239A242A243A243A244A239A245A2462G174MJ在G993同時間內(nèi)對丹參地上部分丹酚酸B含量的影響以510A190A203G48的MJ噴施丹參葉面，在G993同時間分G2047檢測莖葉中的丹酚酸B含量，G13479G7536G16277G32828。實驗G13479G7536G7186G12046，隨處理時間的G5322長，丹參葉和莖中的丹酚酸B含量增加，其中葉中的丹酚酸B含量增加的G5145度G1075G17751莖的G3835。在處理G262H和120H后，丹參葉中丹酚酸B含量分G2047G17751對照增加G111411G26G8和1G25G26G8，莖中丹酚酸B含量分G2047G17751對照增加G11145G26G8和8G25G8。A58A58A59A60A58A59A61A58A59A62A58A59A63A64A64A59A60A64A59A61A64A59A62A64A59A63A58A60A61A61A63A66A60A64A60A58A67A68A71A72A73A75A76A77A78A79A80A114A49A50A51A168A65A170A205A81A52A53A70A54A55A56A23MJA212A208A13A16A3A4A208A209A210A211A21A5A23A6A7A220A221A222A207A81A32A229A229A250A234A228A225A229A37A15A221A233A235A221A229A229A250A224A250A233A228A228A224A250A227A228A250A235A228A221A226A250A230A225A233A230A227A231A232A221A227A233A225A231A221A234A227A234A221A235A211A221A233A228A223A250A230A228A250A226A230A227A233A235A231A250A227A232A250A230A225A229A236A246A240A17A239A246A238A239A240A241A239A242A243A243A244A239A245A24682討論G48G45對植G10301次生產(chǎn)G10301積累的作用與其生理G2163能G7389關(guān)，外施G48G45可以達到逆境的效應(yīng)，G48G45G1075G7171植G10301G13466G14002響應(yīng)G3822種環(huán)境脅迫的共同信號分子A82A83A84A191A84A85A86A87。本G20045研究表明，利用G48G45噴施丹參植G7678葉面，對丹參地上部分G5647酚酸和丹酚酸B的含量積累G18129G7389一定的促進作用，促進作用隨G48G45濃度的增加和時間的G5322G13505而增G5390。藥用植G10301的藥效成分G3835G3822G7171次生代謝產(chǎn)G10301，植G10301次生產(chǎn)G10301的積累G7171與其生境G4506G2011相關(guān)的，一G1135逆境G7389利于次生產(chǎn)G10301的積累A82A85A85A87，G1186生G5589學(xué)的G16294度分G7524，G16780G3822次生產(chǎn)G10301G1075G7171植G10301G19462G5493逆境的植保G13044G12879G10301質(zhì)。研究表明，G993同產(chǎn)地的丹參中的G14038G9354G5627和G8712G9354G5627成分的含量均G7389G7186著差異A88A85A89A90，表明丹參的次生產(chǎn)G10301含量受環(huán)境的調(diào)控G5627。在一G1135研究中利用G48G45作為誘導(dǎo)子誘導(dǎo)培養(yǎng)的G13466G14002積累G11458的產(chǎn)G10301，G3926G726G48G45能夠誘導(dǎo)培養(yǎng)的肉蓯蓉G13466G14002中苯G1069G18267甙G451紅豆杉G13466G14002中的G13055杉G18267G12573G10301質(zhì)含量的增加G15對于培養(yǎng)的丹參毛狀G7693中丹參G18242含量的積累G1075G7389促進作用A88A91A84A85A204A84A85A92A90。G48G45可以G1186植G10301的氣孔進G1849植G10301體G15在G13466G14002質(zhì)中被酯酶G8712G16311為茉莉酸G12573小分子G15實現(xiàn)G19462G5493信號的遠距離G1268導(dǎo)G15并能在植G10301間進行信號交G8981，在植G10301組織的G9094相和氣相中自G11013的快速G12239G2172。本研究G13479G7536G7186G12046，G48G45對丹參葉的作用效應(yīng)G17751莖的G7186著，可能G11013于G48G45直接噴施到葉G7389關(guān)，而在莖中存在距離效應(yīng)和濃度效應(yīng)，相關(guān)機制需進一步研究。丹參通常以G7693G1849藥G15本G20045研究檢測G1114丹參莖葉中G8712G9354G5627G5647酚酸和丹酚酸B的含量均G17751高，未被G48G45處理的G7477G1226G991，葉的丹酚酸B含量為0G17G25G22G8G15莖的丹酚酸B含量為0G17G25G25G8G727經(jīng)G48G45處理后，莖葉的丹酚酸B含量G18129G7389G993同G12255度的提高，G48G45在510A93A94G16510A93A95的濃度范圍內(nèi)G15葉的丹酚酸B含量G17751對照增加G1114G220G17G26G8G1611G26G705，莖中丹酚酸B含量G17751對照增加G1114G22G705G165G26G705，對于外施G48G45對丹參G7693中G1039G16213藥效成分的影響，將另作報道。因此，今后G7389望考慮G48G45在丹參生產(chǎn)中的應(yīng)用問題，在丹參的種植及采收中，應(yīng)注意地上部分的利用，同時G7389必G16213深G1849研究G48G45促進丹酚酸G12573藥效成分積累的機制。參考文獻1ZHG36G50G54G45G15ZHG36G49G42G42G60G15LIG56G39G15G72G87G68G79G17G36G71G89G68G81G70G72G86G76G81G85G72G86G72G68G85G70HG82G73G83HG68G85G80G68G70G82G79G82G74G76G70G68G79G68G70G87G76G89G76G87G76G72G86G68G81G71G69G76G82G86G92G81G87HG72G86G76G86G83G68G87HG90G68G92G82G73G90G68G87G72G85G86G82G79G88G69G79G72G83HG72G81G82G79G76G70G70G82G80G83G82G88G81G71G86G82G73SALVIAMILTIORRHIZAG45G17CHG76G81G55G85G68G71G76G87HG72G85G69G39G85G88G74G869G708中G14621藥G709，2004，G225G708G22G709G726G2241G16G2244G172G39G56G42H，ZHG36G49G42G45G55G17G55HG72G86G87G88G71G92G85G72G89G76G72G90G82G73G86G68G79G89G76G68G81G82G79G76G70G68G70G76G71G86G73G85G82G80G90G68G87G72G85G86G82G79G88G69G79G72G68G70G87G76G89G72G76G81G74G85G72G71G76G72G81G87G86G76G81SALVIAMILTIORRHIZAG39G56G42H，ZHG36G49G42G45G55G17G45G17BG68G86G76G70G48G72G71G76G70G68G79G54G70G76G72G81G70G72G86G68G81G71CG79G76G81G76G70G86G708基礎(chǔ)G2319學(xué)與臨床G709，2000，2510G1614G17G22ZHG50G56L，G49IEG45HG17G55HG72G86G87G88G71G92G85G72G89G76G72G90G82G73G86G68G79G89G76G68G81G82G79G76G70G68G70G76G71BG17G45G17PHG68G85G80G68G70G92G82G73G58G72G86G87CHG76G81G68G708西部藥學(xué)G709，200G26，4G7084G709G72621G26G16220G174ZHG50G56FQ，HG56G36G49G42G54G53，G58G36G49G42G55G17G72G87G68G79G17PG85G72G79G76G80G76G81G68G85G92G86G87G88G71G92G82G81G70HG72G80G76G70G68G79G70G82G80G83G82G86G76G87G76G82G81G86G82G73G79G72G68G89G72G86G82G73G54G68G79G89G76G68G48G76G79G87G76G82G85G85HG76G93G68BG74G72G17G45G17G45G82G88G85G81G68G79G82G73G54HG68G81G39G82G81G74G56G81G76G89G72G85G86G76G87G92G82G73G55CG48G708山東G3835學(xué)學(xué)報G709G17200G26，G221G708G25G709504G1650G25G175LIZG42，G54HIG45G60，ZHG36G49G42ZHG17CG82G80G83G68G85G68G87G76G89G72G68G81G71G68G81G68G79G92G86G76G86G82G73G86G68G79G89G76G68G81G82G79G76G70G68G70G76G71BG76G81G39G68G81G86HG72G81G79G72G68G89G72G86G68G81G71G86G87G72G80G86G69G92HPLCG45G17G45G82G88G85G81G68G79G82G73PHG68G85G80G68G70G72G88G87G76G70G68G79PG85G68G70G87G76G70G72G708藥學(xué)實踐雜志G709，200G26，25G7085G709G726G220G26G16G220G28G17G25G54G70G82G87G87G15IG17G48G17G39G68G87G15G45G17FG17G54G68G79G76G70G92G79G76G70G68G70G76G71G68G81G71HG92G71G85G82G74G72G81G83G72G85G82G91G76G71G72G76G81G68G69G76G82G87G76G70G86G87G85G72G86G86G86G76G74G81G68G79G76G81G74G76G81G83G79G68G81G87G86G17PHG92G87G82G81G16G36G81G81G68G79G72G86G53G72G76G17