《天然藥物化學(xué)教學(xué)》ex(2)

1、整理課件EXP 2. Extraction of “Fen Fang Ji” AlkaloidsEXP 1. The Preparation of Thin-Layer Chromatography (TLC) Silica Gel Plates整理課件1. Purpose 1. Purpose : : Acquire the preparation of TLC silica Acquire the preparation of TLC silica gel plates.gel plates.2. Principle:2. Principle:l Chromatography plates

2、Chromatography plates( (層析板層析板): ): a glass, plastic, a glass, plastic, metal sheet or platemetal sheet or platel AdsorbentsAdsorbents( (吸附劑吸附劑): ): Silica gel H, silica gel G, Silica gel H, silica gel G, silica gel GFsilica gel GF254254, and silica gel HF, and silica gel HF254254. .l AdhesivesAdhes

3、ives( (粘合劑粘合劑): ): CMCNa, Plaster of CMCNa, Plaster of Paris(CaSOParis(CaSO4 4.1/2H.1/2H2 2O), starch(O), starch(淀粉）淀粉）l Activity grade: five gradesActivity grade: five grades Adsorption-Partition3. Apparatus and Reagents3. Apparatus and Reagents Glass plates 10Glass plates 107.5cm, clean and dry 7.

4、5cm, clean and dry silica H for TLC silica H for TLC 0.8% CMC-Na0.8% CMC-NaMoisture content05152538Activity gradeEXP 1. Preparation of Silica Gel TLC Plates 整理課件1. Purpose : Learn to extract total alkaloids from raw materials.2. Principle:u The methods of extraction: reflux extractu Main Chemical co

5、nstituents from Fen Fang Ji: Alkaloidsu Structures of alkaloids: EXP. 2. Extraction of “Fen Fang Ji” Alkaloids 整理課件Procedures for Exp. 1: n It is important to ensure that the glass plates are clean and free grease before use. n The silica H(7g) is mixed with 3 times volumes (20mL) of its own weight of CMC-Na (0.8%) to form mixed slurry.n Spread the slurry quickly on to the plates from one to another, and then shake the plates to form a uniform film.n Transfer the TLC plates to an even plane and dry it in the shade.n Activate the TLC plates at 105oC in an oven for 30 minutes.n Allow th