常用熒光染料探針列表

1、 這是來(lái)自于Salk的一個(gè)比較全的熒光染料列表，這些熒光染料可廣泛用于流式細(xì)胞術(shù)以及熒光顯微鏡技術(shù)，匯集了各種熒光染料的特性，方便大家查找?？筛鶕?jù)實(shí)際所用的檢測(cè)平臺(tái)、染料的最大激發(fā)光波長(zhǎng)和最大發(fā)射光波長(zhǎng)來(lái)選擇合適的熒光染料用于實(shí)驗(yàn)。請(qǐng)注意這上面所顯示的顏色可能會(huì)由于所用瀏覽器不同而有所不同，他們只是一個(gè)與實(shí)際顏色的近似值。染料名稱Excitation(nm)Emission(nm)分子量備注信息Reactive and conjugated probesHydroxycoumarin325386331Succinimidyl esterAminocoumarin350445330Su

2、ccinimidyl esterMethoxycoumarin360410317Succinimidyl esterCascade Blue(375);401423596HydrazidePacific Blue403455406MaleimidePacific Orange403551  Lucifer yellow425528  NBD466539294NBD-XR-Phycoerythrin (PE)480;565578240 k PE-Cy5 conjugates480;565;650670 aka Cychrome, R67

3、0, Tri-Color, Quantum RedPE-Cy7 conjugates480;565;743767  Red 613480;565613 PE-Texas RedPerCP490675 Peridinin chlorphyll proteinTruRed490,675695 PerCP-Cy5.5 conjugateFluorX494520587GE HealthcareFluorescein495519389FITC; pH sensitiveBODIPY-FL503512  TRITC547572444TR

4、ITCX-Rhodamine570576548XRITCLissamine Rhodamine B570590  Texas Red589615625Sulfonyl chlorideAllophycocyanin (APC)650660104 k APC-Cy7 conjugates650;755767 PharRedAlexa Fluor系列熒光染料Alexa Fluor 350343442410 Alexa Fluor 4054014211028 Alexa Fluor 430434540702 Alexa Fluor

5、 488499519643QY 0.92Alexa Fluor 500503525700 Alexa Fluor 514517542714 Alexa Fluor 532530555724QY 0.61Alexa Fluor 5465615721079QY 0.79Alexa Fluor 5555535681250QY 0.1Alexa Fluor 568579603792QY 0.69Alexa Fluor 594591618820QY 0.66Alexa Fluor 6106106291285 Alexa Fluor 6336326481200 Al

6、exa Fluor 6476526681300QY 0.33Alexa Fluor 6606636911100 Alexa Fluor 6806807021150 Alexa Fluor 7006967191400 Alexa Fluor 7507527761300 Alexa Fluor 7907828041750 Cy系列熒光染料Cy2489506714QY 0.12Cy3(512);550570;(615)767QY 0.15Cy3B558572;(620)658QY 0.67Cy3.5581594;(640)1102QY 0.15Cy5

7、(625);650670792QY 0.28Cy5.56756941128QY 0.23Cy7743767818QY 0.28核酸相關(guān)探針染料Hoechst 33342343483616 AT-selective DAPI345455 AT-selective Hoechst 33258345478624AT-selective SYTOX Blue431480400DNA Chromomycin A3445575 CG-selective Mithramycin445575  YOYO#160;Ethidium Bromide

8、493620394  Acridine Orange503530/640 DNA/RNASYTOX Green504523600 DNA TOTO-1, TO-PRO-1509533 Vital stain, TOTO: Cyanine DimerTO-PRO: Cyanine MonomerThiazole Orange510530  Propidium Iodide (PI)536617668.4 LDS 751543;590712;607472 DNA (543ex/712em), RNA (590ex/607em) 7-AAD

9、546647 7-aminoactinomycin D, CG-selectiveSYTOX Orange547570500 DNA TOTO-3, TO-PRO-3642661  DRAQ5647681,697413usable excitation down to 488細(xì)胞功能探針I(yè)ndo-1361/330490/4051010AM ester. Low/High Ca+,Fluo-3506526855AM ester. pH > 6DCFH5055355292'7'Dichorodihydrofluorescein, oxidized

10、 formDHR505534346Dihydrorhodamine 123, oxidized form,light catalyzes oxidationSNARF548/579587/635 pH 6/9熒光蛋白    QYBRPS Y66H360442     Y66F360508     EBFP380440 0.180.27 monomerEBFP2383448  20 mon

11、omerAzurite383447  15 monomerGFPuv385508     T-Sapphire399511 0.602625weak dimerCerulean433475 0.622736weak dimermCFP433475 0.401364monomerECFP434477 0.153  CyPet435477 0.511859weak dimerY66W436485    

12、0;mKeima-Red440620 0.243 monomerTagCFP458480  29 dimerAmCyan1458489 0.7529 tetramermTFP1462492  54 dimerS65A471504     Midoriishi Cyan472495 0.925 dimerWild Type GFP396,47550826k0.77   S65C479507 

13、;    TurboGFP48250226 k0.5337 dimerTagGFP482505  34 monomerS65L484510     Emerald487509 0.68390.69weak dimer; (Invitrogen)S65T488511     EGFP48850726k 0.6034174weak dimerAzami Green492505 0.7441 

14、monomerZsGreen1493505105k0.9140 tetramerTagYFP508524  47 monomerEYFP51452726k0.615160weak dimerTopaz514527  57 monomerVenus515528 0.575315weak dimermCitrine516529 0.765949monomerYPet517530 0.778049weak dimerTurboYFP52553826 k0.531.65 dimerZsYell

15、ow1529539 0.6513 tetramerKusabira Orange548559 0.6031 monomermOrange548562 0.69499monomermKO548559 0.6031122monomerTurboRFP55357426 k0.6762 dimertdTomato554581 0.699598tandem dimerTagRFP555584  50 monomerDsRed monomer55658628k0.13.516monomerDsRe

16、d2 ("RFP")563582110k0.5524  mStrawberry574596 0.292615monomerTurboFP60257460226 k0.3526 dimerAsRed2576592110k0.2113 tetramermRFP158460730k0.25  monomerJ-Red584610 0.208.813dimermCherry587610 0.221696monomerHcRed158861852k0.030.6 dimerKatush

17、a588635  23 dimermKate (TagFP635)588635  15 monomerTurboFP63558863526 k0.3422 dimermPlum590649 0.104.153 mRaspberry598625 0.1513 monomer; faster photobleach than mPlum其他探針Monochlorobimane380461226Glutathione probeCalcein496517623pH > 5 Ex: Peak excitation wavelength (nm) Em: Peak emission wavelength