鉛負(fù)荷與T細(xì)胞亞群表達(dá)的相關(guān)性研究

1、鉛負(fù)荷與T細(xì)胞亞群表達(dá)的相關(guān)性研究         11-05-19 09:55:00     編輯：studa20               作者：孟慶禾 李世光 付葵 孔慶浩 李琳【摘要】  目的 評(píng)估鉛負(fù)荷兒童T細(xì)胞亞群表達(dá)以及鉛水平與T細(xì)胞亞群細(xì)胞表達(dá)的關(guān)系。方法 采用石墨爐原子吸收光譜法對(duì)太原市環(huán)境污染較嚴(yán)重地區(qū)的3

2、6歲兒童120例進(jìn)行血鉛水平測(cè)定及免疫熒光法檢測(cè)T細(xì)胞亞群;根據(jù)血鉛值將兒童分為<0.24 mol/L組、0.24 mol/L和0.48 mol/L組，分別為54、40和26例，比較不同血鉛水平兒童T細(xì)胞亞群陽性細(xì)胞比例及血鉛水平與T細(xì)胞亞群的關(guān)系。 結(jié)果 血鉛水平0.24，<0.48 mol/L組與<0.24 mol/L組T細(xì)胞亞群CD3、CD4、CD8、 CD4/CD8和 B陽性細(xì)胞百分比比較，差異無統(tǒng)計(jì)學(xué)意義;血鉛0.48 mol/L組CD3、CD4/CD8值低于0.24，<0.48 mol/L組，CD8值高于0.24，<0.48 mol/L組，差異有統(tǒng)計(jì)學(xué)

3、意義(P<0.01，P<0.05);血鉛0.48 mol/L，血鉛水平與T細(xì)胞亞群CD3、 CD4/CD8呈負(fù)相關(guān)，與 CD8呈正相關(guān)。 結(jié)論 高鉛水平對(duì)小兒T細(xì)胞亞群表達(dá)有不良影響。 【關(guān)鍵詞】  鉛負(fù)荷; T細(xì)胞亞群; 相關(guān)性【Abstract】 Objective To assess the effect of blood lead level on Tlymphocyte subpopulation expression and the association of Tlymphocyte subpopulation expression with thresho

4、ld limit value of blood lead among children. Methods Blood lead level was measured with graphite furnace atomic absorption spectroscopy and the expression of Tlymphocyte subpopulation was measured with immunofluorescence method. Tlymphocyte subpopulation express was compared between groups with diff

5、erent blood levels and correlation between blood lead level and expression of Tlymphocyte subpopulation was analyzed. A total of 120 children, (64 boys and 56 girls), aged 36 years with a mean age of 4.5 1.5 years, were selected from the suburbs of Taiyuan city between May 2003 and October 2003. Acc

6、ording to their blood lead levels the enrolled children were assigned into one of the three groups: 26 in Group I with a blood lead level of 0.48 mol/L, 40 in Group 2 with a lead level of 0.24 mol/L but <0.48 mol/L, and 54 in Group 3 with a lead level of <0.24 mol/L. Results There were no sign

7、ificant differences in CD3, CD4 and CD8 cell expression, CD4/CD8 cell ratio, and Blymphocyte positive rate between Group 2 and Group 3. However, CD3 cell expression and CD4/CD8 cell ratio were lower (P<0.01), and CD8 cell expression was higher (P<0.05) in Group 1 when compared with Group 2. Bl

8、ood level was negatively correlated with CD3 cell expression and CD4/CD8 ratio, but positively correlated with CD8 cell expression in Group 1. Conclusion High blood lead level has adverse impact on Tlymphocyte subpopulation expression.【Key words】 Lead exposure; Tlymphocyte subpopulation; Blood; Pres

9、chool children鉛可能是一種潛在性的免疫調(diào)節(jié)因子，長(zhǎng)期低鉛染毒可影響大鼠的細(xì)胞免疫功能1。T細(xì)胞亞群是一個(gè)綜合的免疫指標(biāo)，便于評(píng)估機(jī)體免疫平衡狀態(tài)和整體的免疫能力，有很好的臨床應(yīng)用性。鑒于國(guó)內(nèi)研究鉛負(fù)荷與T細(xì)胞亞群表達(dá)關(guān)系的報(bào)道甚少，本研究擬通過兒童血鉛含量與T細(xì)胞亞群的表達(dá)揭示環(huán)境鉛污染對(duì)免疫功能的影響。根據(jù)鉛分界值觀察鉛負(fù)荷兒童T細(xì)胞亞群的變化，以及鉛濃度與T細(xì)胞亞群表達(dá)的關(guān)系，旨在探討T細(xì)胞亞群表達(dá)在鉛中毒兒童中變化及其意義。對(duì)象與方法一、對(duì)象選擇2005年5月10月太原市環(huán)境污染較嚴(yán)重的地區(qū)120例兒童，男童62例，女童58例，年齡36歲，平均年齡(4.5±1.5

10、)歲，所有兒童近期內(nèi)無其他感染性疾病。二、方法1.血鉛測(cè)定與分組：采用石墨爐原子吸收光譜法(美國(guó)Buck210原子吸收光譜儀)，測(cè)定過程嚴(yán)格執(zhí)行實(shí)驗(yàn)室內(nèi)部質(zhì)量控制，并參加中科院環(huán)境衛(wèi)生監(jiān)督所盲樣質(zhì)控。血鉛以0.24 mol/L和0.48 mol/L為分界值分為3組。2.T細(xì)胞亞群的測(cè)定：采用免疫熒光法2，3取患兒肝素抗凝血用淋巴細(xì)胞分離液按常規(guī)配制單個(gè)核細(xì)胞，懸液分加于聚乙烯V型塑料板4個(gè)孔內(nèi)，每50 l繼加等量的120鼠抗人單克隆WuT3、WuT4、WuT8、WuB152(B細(xì)胞)，溫育洗滌后加羊抗鼠IgG熒光抗體50l,同前加溫育洗滌后用熒光顯微鏡計(jì)數(shù)。每孔計(jì)數(shù)200個(gè)細(xì)胞左右，計(jì)算陽性細(xì)

11、胞的百分比。3.統(tǒng)計(jì)學(xué)處理：采用SPSS13.0軟件建立數(shù)據(jù)庫，計(jì)量資料采用t檢驗(yàn)，血鉛與T細(xì)胞亞群的關(guān)系采用直線相關(guān)分析。結(jié) 果一、不同血鉛水平T細(xì)胞亞群的表達(dá)血鉛水平<0.24 mol/L與0.24 mol/L組T細(xì)胞亞群CD3、CD4、CD8、 CD4/CD8和 B陽性細(xì)胞百分比比較，差異無統(tǒng)計(jì)學(xué)意義;血鉛0.48 mol/L組CD3、CD4/CD8值低于0.24 mol/L組，CD8值高于0.24 mol/L組，差異有統(tǒng)計(jì)學(xué)意義(P<0.01，P<0.05)，見表1。二、血鉛水平與T細(xì)胞亞群的關(guān)系血鉛水平與T細(xì)胞亞群的相關(guān)分析顯示，血鉛0.48 mol/L，血鉛水平與

12、T細(xì)胞亞群CD3、 CD4/CD8呈負(fù)相關(guān)，相關(guān)系數(shù)分別為-0.689、-0.674(P<0.01)，血鉛水平越高CD3、 CD4/CD8水平越低;血鉛水平與T細(xì)胞亞群CD8呈正相關(guān),相關(guān)系數(shù)0.748(P<0.01)，血鉛水平越高CD8水平越高;血鉛水平<0.48 mol/L組與T細(xì)胞亞群無相關(guān)性，見表2。表1 血鉛水平與T細(xì)胞亞群的表達(dá)表2 血鉛與T細(xì)胞亞群的關(guān)系討 論鉛是對(duì)人體有害的重金屬元素，廣泛存在于環(huán)境中，兒童接觸過量鉛，不但可導(dǎo)致發(fā)育遲緩，而且還可以造成氣管功能的異常。鉛主要通過呼吸道和消化道攝入體內(nèi)，90 %鉛沉積于骨骼作為儲(chǔ)存池，其余隨血流分布到全身各器官和組織，產(chǎn)